Combined effect of water activity and pH on the inhibition of Escherichia coli by nisin

The Doehlert design and surface response methodology were used to study the influence of pH and water activity (aw) on Escherichia coli inhibition by nisin. Combining stress factors at levels where they are not inhibitory by themselves, a reduction of E. coli survival fraction can be achieved with l...

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Autores principales:	Cerrutti, Patricia, Terebiznik, Mauricio R., Jagus de Kanterewicz, Rosa Juana, Pilosof, Ana María Renata
Publicado:	2001
Materias:	bacterial cell bacterial count bacterial growth cell survival colony forming unit food safety growth inhibition nisin pH water flow Bacteria (microorganisms) Escherichia coli
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0362028X_v64_n10_p1510_Cerrutti http://hdl.handle.net/20.500.12110/paper_0362028X_v64_n10_p1510_Cerrutti
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_0362028X_v64_n10_p1510_Cerrutti
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spelling	paper:paper_0362028X_v64_n10_p1510_Cerrutti2023-06-08T15:34:59Z Combined effect of water activity and pH on the inhibition of Escherichia coli by nisin Cerrutti, Patricia Terebiznik, Mauricio R. Jagus de Kanterewicz, Rosa Juana Pilosof, Ana María Renata bacterial cell bacterial count bacterial growth cell survival colony forming unit food safety growth inhibition nisin pH water flow Bacteria (microorganisms) Escherichia coli Escherichia coli The Doehlert design and surface response methodology were used to study the influence of pH and water activity (aw) on Escherichia coli inhibition by nisin. Combining stress factors at levels where they are not inhibitory by themselves, a reduction of E. coli survival fraction can be achieved with lower nisin doses than in a single nisin treatment. For all the pH values assayed, a synergistic effect of aw and nisin concentration was detected, and the isoresponse lines showed the existence of an area of maximum inhibition. Factors that reduced viable cell counts by 4 to 5 log cycles were 1,000 to 1,400 IU of nisin per ml at pH 5.5 to 6.5 and a water activity of 0.97 and 0,98. The addition of different ionic and nonionic solutes to control aw suggested that the effect of aw in the inhibitory action of nisin on E. coli cells was not solute-specific. The use of the Doehlert experimental design was effective to determine the optimal combination of stress factors, as well as to point out the most important variables that affected E. coli inhibition. Fil:Cerrutti, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Terebiznik, M.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Jagus, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pilosof, A.M.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2001 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0362028X_v64_n10_p1510_Cerrutti http://hdl.handle.net/20.500.12110/paper_0362028X_v64_n10_p1510_Cerrutti
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	bacterial cell bacterial count bacterial growth cell survival colony forming unit food safety growth inhibition nisin pH water flow Bacteria (microorganisms) Escherichia coli Escherichia coli
spellingShingle	bacterial cell bacterial count bacterial growth cell survival colony forming unit food safety growth inhibition nisin pH water flow Bacteria (microorganisms) Escherichia coli Escherichia coli Cerrutti, Patricia Terebiznik, Mauricio R. Jagus de Kanterewicz, Rosa Juana Pilosof, Ana María Renata Combined effect of water activity and pH on the inhibition of Escherichia coli by nisin
topic_facet	bacterial cell bacterial count bacterial growth cell survival colony forming unit food safety growth inhibition nisin pH water flow Bacteria (microorganisms) Escherichia coli Escherichia coli
description	The Doehlert design and surface response methodology were used to study the influence of pH and water activity (aw) on Escherichia coli inhibition by nisin. Combining stress factors at levels where they are not inhibitory by themselves, a reduction of E. coli survival fraction can be achieved with lower nisin doses than in a single nisin treatment. For all the pH values assayed, a synergistic effect of aw and nisin concentration was detected, and the isoresponse lines showed the existence of an area of maximum inhibition. Factors that reduced viable cell counts by 4 to 5 log cycles were 1,000 to 1,400 IU of nisin per ml at pH 5.5 to 6.5 and a water activity of 0.97 and 0,98. The addition of different ionic and nonionic solutes to control aw suggested that the effect of aw in the inhibitory action of nisin on E. coli cells was not solute-specific. The use of the Doehlert experimental design was effective to determine the optimal combination of stress factors, as well as to point out the most important variables that affected E. coli inhibition.
author	Cerrutti, Patricia Terebiznik, Mauricio R. Jagus de Kanterewicz, Rosa Juana Pilosof, Ana María Renata
author_facet	Cerrutti, Patricia Terebiznik, Mauricio R. Jagus de Kanterewicz, Rosa Juana Pilosof, Ana María Renata
author_sort	Cerrutti, Patricia
title	Combined effect of water activity and pH on the inhibition of Escherichia coli by nisin
title_short	Combined effect of water activity and pH on the inhibition of Escherichia coli by nisin
title_full	Combined effect of water activity and pH on the inhibition of Escherichia coli by nisin
title_fullStr	Combined effect of water activity and pH on the inhibition of Escherichia coli by nisin
title_full_unstemmed	Combined effect of water activity and pH on the inhibition of Escherichia coli by nisin
title_sort	combined effect of water activity and ph on the inhibition of escherichia coli by nisin
publishDate	2001
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0362028X_v64_n10_p1510_Cerrutti http://hdl.handle.net/20.500.12110/paper_0362028X_v64_n10_p1510_Cerrutti
work_keys_str_mv	AT cerruttipatricia combinedeffectofwateractivityandphontheinhibitionofescherichiacolibynisin AT terebiznikmauricior combinedeffectofwateractivityandphontheinhibitionofescherichiacolibynisin AT jagusdekanterewiczrosajuana combinedeffectofwateractivityandphontheinhibitionofescherichiacolibynisin AT pilosofanamariarenata combinedeffectofwateractivityandphontheinhibitionofescherichiacolibynisin
_version_	1768543562788503552

Combined effect of water activity and pH on the inhibition of Escherichia coli by nisin

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