Enzymatic Hydrolysis of Soy Protein Isolates : DSC study

The aim of this work was to analyze the possible use of differential scanning calorimetry (DSC) as a method to study the process of protein modifications during enzymatic hydrolysis. Results of the enzymatic hydrolysis of soy protein showed significant differences in the values of maximum deflection...

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Autores principales: Molina Ortiz, Sara E., Añón, María Cristina
Formato: Articulo
Lenguaje:Inglés
Publicado: 2001
Materias:
Bioquímica
DSC
hydrolysis
soy proteins
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/140987
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-140987
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Bioquímica
DSC
hydrolysis
soy proteins
spellingShingle Bioquímica
DSC
hydrolysis
soy proteins
Molina Ortiz, Sara E.
Añón, María Cristina
Enzymatic Hydrolysis of Soy Protein Isolates : DSC study
topic_facet Bioquímica
DSC
hydrolysis
soy proteins
description The aim of this work was to analyze the possible use of differential scanning calorimetry (DSC) as a method to study the process of protein modifications during enzymatic hydrolysis. Results of the enzymatic hydrolysis of soy protein showed significant differences in the values of maximum deflection temperature (Tp), heat of reaction (ΔH), and width at half peak height (ΔT1/2), between DSC curves corresponding to the substrate, or zerotime of hydrolysis, and those of the hydrolysates obtained by the action of cucurbita and pomiferin enzymes. DSC curve changes mentioned were explained by the use of gel-filtration chromatography, denaturing electrophoresis and surface hydrophobicity of the hydrolysis products obtained at 30 min of reaction.
format Articulo
Articulo
author Molina Ortiz, Sara E.
Añón, María Cristina
author_facet Molina Ortiz, Sara E.
Añón, María Cristina
author_sort Molina Ortiz, Sara E.
title Enzymatic Hydrolysis of Soy Protein Isolates : DSC study
title_short Enzymatic Hydrolysis of Soy Protein Isolates : DSC study
title_full Enzymatic Hydrolysis of Soy Protein Isolates : DSC study
title_fullStr Enzymatic Hydrolysis of Soy Protein Isolates : DSC study
title_full_unstemmed Enzymatic Hydrolysis of Soy Protein Isolates : DSC study
title_sort enzymatic hydrolysis of soy protein isolates : dsc study
publishDate 2001
url http://sedici.unlp.edu.ar/handle/10915/140987
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AT anonmariacristina enzymatichydrolysisofsoyproteinisolatesdscstudy
bdutipo_str Repositorios
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