In-House Validation of Rapid Detection PCRs for Bacterial Pathogens Causing Infant Diarrhea

In Argentina, conventional culture methods for the isolation of diarrheal bacteria continue to be the most widely used form of diagnosis in many clinical laboratories. In this work we validated 11 in-house real-time polymerasechain reactions (PCRs) assays for the specific and rapid detection of Salm...

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Autores principales: Londero, Alejandra, Leotta, Gerardo Aníbal, Brusa, Victoria, Costa, Magdalena, Golijow, Carlos Daniel, Gutkind, Gabriel Osvaldo, Gonzalez, E., Galli, Lucía
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Bioquímica
Bacteria
Detection
Diarrheal
RT-PCR
Validation
Infant
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/100515
https://ri.conicet.gov.ar/11336/48103
https://www.omicsonline.org/open-access/inhouse-validation-of-rapid-detection-pcrs-for-bacterial-pathogens-causing-infant-diarrhea-2161-0703-1000268-97066.html
https://www.hilarispublisher.com/open-access/inhouse-validation-of-rapid-detection-pcrs-for-bacterial-pathogens-causinginfant-diarrhea-2161-0703-1000268.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-100515
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Bioquímica
Bacteria
Detection
Diarrheal
RT-PCR
Validation
Infant
spellingShingle Bioquímica
Bacteria
Detection
Diarrheal
RT-PCR
Validation
Infant
Londero, Alejandra
Leotta, Gerardo Aníbal
Brusa, Victoria
Costa, Magdalena
Golijow, Carlos Daniel
Gutkind, Gabriel Osvaldo
Gonzalez, E.
Galli, Lucía
In-House Validation of Rapid Detection PCRs for Bacterial Pathogens Causing Infant Diarrhea
topic_facet Bioquímica
Bacteria
Detection
Diarrheal
RT-PCR
Validation
Infant
description In Argentina, conventional culture methods for the isolation of diarrheal bacteria continue to be the most widely used form of diagnosis in many clinical laboratories. In this work we validated 11 in-house real-time polymerasechain reactions (PCRs) assays for the specific and rapid detection of Salmonella spp., Shigella spp., enteroinvasive E. coli, enteropathogenic E. coli, enterotoxigenic E. coli, Shiga toxin-producing E. coli, E. coli O157, Cronobacter sakazakii, Campylobacter jejuni, Campylobacter coli, Vibrio cholera and Clostridium difficile. The sensitivity of the assays was less than 100 CFU/ml for all the studied pathogens; selectivity and specificity were 100% in all cases and robustness was optimal. These PCR methods could be used to accurately detect the main bacterial causes of infant gastroenteritis.
format Articulo
Articulo
author Londero, Alejandra
Leotta, Gerardo Aníbal
Brusa, Victoria
Costa, Magdalena
Golijow, Carlos Daniel
Gutkind, Gabriel Osvaldo
Gonzalez, E.
Galli, Lucía
author_facet Londero, Alejandra
Leotta, Gerardo Aníbal
Brusa, Victoria
Costa, Magdalena
Golijow, Carlos Daniel
Gutkind, Gabriel Osvaldo
Gonzalez, E.
Galli, Lucía
author_sort Londero, Alejandra
title In-House Validation of Rapid Detection PCRs for Bacterial Pathogens Causing Infant Diarrhea
title_short In-House Validation of Rapid Detection PCRs for Bacterial Pathogens Causing Infant Diarrhea
title_full In-House Validation of Rapid Detection PCRs for Bacterial Pathogens Causing Infant Diarrhea
title_fullStr In-House Validation of Rapid Detection PCRs for Bacterial Pathogens Causing Infant Diarrhea
title_full_unstemmed In-House Validation of Rapid Detection PCRs for Bacterial Pathogens Causing Infant Diarrhea
title_sort in-house validation of rapid detection pcrs for bacterial pathogens causing infant diarrhea
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/100515
https://ri.conicet.gov.ar/11336/48103
https://www.omicsonline.org/open-access/inhouse-validation-of-rapid-detection-pcrs-for-bacterial-pathogens-causing-infant-diarrhea-2161-0703-1000268-97066.html
https://www.hilarispublisher.com/open-access/inhouse-validation-of-rapid-detection-pcrs-for-bacterial-pathogens-causinginfant-diarrhea-2161-0703-1000268.pdf
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