In vitro effects of endosulfan-based insecticides on mammalian sperm

Endosulfan is an organochloride insecticide extensively used in several countries to protect crops from pests. As several studies indicate that endosulfan can affect human and animal development, the aim of this study was to analyse whether sperm parameters and the process of chromatin decondensatio...

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Autores principales:	Sánchez, M.C., Alvarez Sedó, C., Chaufan, G.R., Romanato, M., Da Cuña, R., Lo Nostro, F., Calvo, J.C., Fontana, V.
Formato:	JOUR
Materias:	endosulfan glutathione glutathione transferase heparin superoxide dismutase acrosome animal experiment Article chromatin controlled study DNA fragmentation enzyme activity epididymis in vitro study lipid peroxidation male mammal mouse nonhuman oxidative stress priority journal seminal plasma spermatozoon spermatozoon motility spermatozoon penetration transmission electron microscopy TUNEL assay
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_2045452X_v7_n1_p117_Sanchez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_2045452X_v7_n1_p117_Sanchez
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spelling	todo:paper_2045452X_v7_n1_p117_Sanchez2023-10-03T16:38:32Z In vitro effects of endosulfan-based insecticides on mammalian sperm Sánchez, M.C. Alvarez Sedó, C. Chaufan, G.R. Romanato, M. Da Cuña, R. Lo Nostro, F. Calvo, J.C. Fontana, V. endosulfan glutathione glutathione transferase heparin superoxide dismutase acrosome animal experiment Article chromatin controlled study DNA fragmentation enzyme activity epididymis in vitro study lipid peroxidation male mammal mouse nonhuman oxidative stress priority journal seminal plasma spermatozoon spermatozoon motility spermatozoon penetration transmission electron microscopy TUNEL assay Endosulfan is an organochloride insecticide extensively used in several countries to protect crops from pests. As several studies indicate that endosulfan can affect human and animal development, the aim of this study was to analyse whether sperm parameters and the process of chromatin decondensation could be altered by endosulfan in mice sperm. Spermatozoa from cauda epididymis were obtained from mature male mice and incubated in the presence of two commercial formulations (CFs) of endosulfan (Master® and Zebra Ciagro®) or the active ingredient (AI) alone. A significant decrease in the percentage motility and viability of spermatozoa with respect to controls was found. In vitro decondensation was performed in the presence of glutathione and heparin. Spermatozoa incubated with the AI, endosulfan Master® and endosulfan Zebra Ciagro® showed an increase in chromatin decondensation. In addition, the TUNEL assay showed that DNA fragmentation was significantly higher when sperm were incubated with either one of the CFs when compared to the AI or controls. The ultrastructure analysis of sperm cells showed evident changes in the structure of the plasma and acrosome membranes of sperm incubated with endosulfan AI or the CFs. These results suggest that endosulfan can affect sperm integrity and in vitro chromatin decondensation as well as DNA fragmentation. © 2017 The Royal Society of Chemistry. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_2045452X_v7_n1_p117_Sanchez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	endosulfan glutathione glutathione transferase heparin superoxide dismutase acrosome animal experiment Article chromatin controlled study DNA fragmentation enzyme activity epididymis in vitro study lipid peroxidation male mammal mouse nonhuman oxidative stress priority journal seminal plasma spermatozoon spermatozoon motility spermatozoon penetration transmission electron microscopy TUNEL assay
spellingShingle	endosulfan glutathione glutathione transferase heparin superoxide dismutase acrosome animal experiment Article chromatin controlled study DNA fragmentation enzyme activity epididymis in vitro study lipid peroxidation male mammal mouse nonhuman oxidative stress priority journal seminal plasma spermatozoon spermatozoon motility spermatozoon penetration transmission electron microscopy TUNEL assay Sánchez, M.C. Alvarez Sedó, C. Chaufan, G.R. Romanato, M. Da Cuña, R. Lo Nostro, F. Calvo, J.C. Fontana, V. In vitro effects of endosulfan-based insecticides on mammalian sperm
topic_facet	endosulfan glutathione glutathione transferase heparin superoxide dismutase acrosome animal experiment Article chromatin controlled study DNA fragmentation enzyme activity epididymis in vitro study lipid peroxidation male mammal mouse nonhuman oxidative stress priority journal seminal plasma spermatozoon spermatozoon motility spermatozoon penetration transmission electron microscopy TUNEL assay
description	Endosulfan is an organochloride insecticide extensively used in several countries to protect crops from pests. As several studies indicate that endosulfan can affect human and animal development, the aim of this study was to analyse whether sperm parameters and the process of chromatin decondensation could be altered by endosulfan in mice sperm. Spermatozoa from cauda epididymis were obtained from mature male mice and incubated in the presence of two commercial formulations (CFs) of endosulfan (Master® and Zebra Ciagro®) or the active ingredient (AI) alone. A significant decrease in the percentage motility and viability of spermatozoa with respect to controls was found. In vitro decondensation was performed in the presence of glutathione and heparin. Spermatozoa incubated with the AI, endosulfan Master® and endosulfan Zebra Ciagro® showed an increase in chromatin decondensation. In addition, the TUNEL assay showed that DNA fragmentation was significantly higher when sperm were incubated with either one of the CFs when compared to the AI or controls. The ultrastructure analysis of sperm cells showed evident changes in the structure of the plasma and acrosome membranes of sperm incubated with endosulfan AI or the CFs. These results suggest that endosulfan can affect sperm integrity and in vitro chromatin decondensation as well as DNA fragmentation. © 2017 The Royal Society of Chemistry.
format	JOUR
author	Sánchez, M.C. Alvarez Sedó, C. Chaufan, G.R. Romanato, M. Da Cuña, R. Lo Nostro, F. Calvo, J.C. Fontana, V.
author_facet	Sánchez, M.C. Alvarez Sedó, C. Chaufan, G.R. Romanato, M. Da Cuña, R. Lo Nostro, F. Calvo, J.C. Fontana, V.
author_sort	Sánchez, M.C.
title	In vitro effects of endosulfan-based insecticides on mammalian sperm
title_short	In vitro effects of endosulfan-based insecticides on mammalian sperm
title_full	In vitro effects of endosulfan-based insecticides on mammalian sperm
title_fullStr	In vitro effects of endosulfan-based insecticides on mammalian sperm
title_full_unstemmed	In vitro effects of endosulfan-based insecticides on mammalian sperm
title_sort	in vitro effects of endosulfan-based insecticides on mammalian sperm
url	http://hdl.handle.net/20.500.12110/paper_2045452X_v7_n1_p117_Sanchez
work_keys_str_mv	AT sanchezmc invitroeffectsofendosulfanbasedinsecticidesonmammaliansperm AT alvarezsedoc invitroeffectsofendosulfanbasedinsecticidesonmammaliansperm AT chaufangr invitroeffectsofendosulfanbasedinsecticidesonmammaliansperm AT romanatom invitroeffectsofendosulfanbasedinsecticidesonmammaliansperm AT dacunar invitroeffectsofendosulfanbasedinsecticidesonmammaliansperm AT lonostrof invitroeffectsofendosulfanbasedinsecticidesonmammaliansperm AT calvojc invitroeffectsofendosulfanbasedinsecticidesonmammaliansperm AT fontanav invitroeffectsofendosulfanbasedinsecticidesonmammaliansperm
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In vitro effects of endosulfan-based insecticides on mammalian sperm

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