In vitro effects of endosulfan-based insecticides on mammalian sperm

Endosulfan is an organochloride insecticide extensively used in several countries to protect crops from pests. As several studies indicate that endosulfan can affect human and animal development, the aim of this study was to analyse whether sperm parameters and the process of chromatin decondensatio...

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Autores principales: Sánchez, M.C., Alvarez Sedó, C., Chaufan, G.R., Romanato, M., Da Cuña, R., Lo Nostro, F., Calvo, J.C., Fontana, V.
Formato: JOUR
Materias:
endosulfan
glutathione
glutathione transferase
heparin
superoxide dismutase
acrosome
animal experiment
Article
chromatin
controlled study
DNA fragmentation
enzyme activity
epididymis
in vitro study
lipid peroxidation
male
mammal
mouse
nonhuman
oxidative stress
priority journal
seminal plasma
spermatozoon
spermatozoon motility
spermatozoon penetration
transmission electron microscopy
TUNEL assay
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_2045452X_v7_n1_p117_Sanchez
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_2045452X_v7_n1_p117_Sanchez
record_format dspace
spelling todo:paper_2045452X_v7_n1_p117_Sanchez2023-10-03T16:38:32Z In vitro effects of endosulfan-based insecticides on mammalian sperm Sánchez, M.C. Alvarez Sedó, C. Chaufan, G.R. Romanato, M. Da Cuña, R. Lo Nostro, F. Calvo, J.C. Fontana, V. endosulfan glutathione glutathione transferase heparin superoxide dismutase acrosome animal experiment Article chromatin controlled study DNA fragmentation enzyme activity epididymis in vitro study lipid peroxidation male mammal mouse nonhuman oxidative stress priority journal seminal plasma spermatozoon spermatozoon motility spermatozoon penetration transmission electron microscopy TUNEL assay Endosulfan is an organochloride insecticide extensively used in several countries to protect crops from pests. As several studies indicate that endosulfan can affect human and animal development, the aim of this study was to analyse whether sperm parameters and the process of chromatin decondensation could be altered by endosulfan in mice sperm. Spermatozoa from cauda epididymis were obtained from mature male mice and incubated in the presence of two commercial formulations (CFs) of endosulfan (Master® and Zebra Ciagro®) or the active ingredient (AI) alone. A significant decrease in the percentage motility and viability of spermatozoa with respect to controls was found. In vitro decondensation was performed in the presence of glutathione and heparin. Spermatozoa incubated with the AI, endosulfan Master® and endosulfan Zebra Ciagro® showed an increase in chromatin decondensation. In addition, the TUNEL assay showed that DNA fragmentation was significantly higher when sperm were incubated with either one of the CFs when compared to the AI or controls. The ultrastructure analysis of sperm cells showed evident changes in the structure of the plasma and acrosome membranes of sperm incubated with endosulfan AI or the CFs. These results suggest that endosulfan can affect sperm integrity and in vitro chromatin decondensation as well as DNA fragmentation. © 2017 The Royal Society of Chemistry. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_2045452X_v7_n1_p117_Sanchez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic endosulfan
glutathione
glutathione transferase
heparin
superoxide dismutase
acrosome
animal experiment
Article
chromatin
controlled study
DNA fragmentation
enzyme activity
epididymis
in vitro study
lipid peroxidation
male
mammal
mouse
nonhuman
oxidative stress
priority journal
seminal plasma
spermatozoon
spermatozoon motility
spermatozoon penetration
transmission electron microscopy
TUNEL assay
spellingShingle endosulfan
glutathione
glutathione transferase
heparin
superoxide dismutase
acrosome
animal experiment
Article
chromatin
controlled study
DNA fragmentation
enzyme activity
epididymis
in vitro study
lipid peroxidation
male
mammal
mouse
nonhuman
oxidative stress
priority journal
seminal plasma
spermatozoon
spermatozoon motility
spermatozoon penetration
transmission electron microscopy
TUNEL assay
Sánchez, M.C.
Alvarez Sedó, C.
Chaufan, G.R.
Romanato, M.
Da Cuña, R.
Lo Nostro, F.
Calvo, J.C.
Fontana, V.
In vitro effects of endosulfan-based insecticides on mammalian sperm
topic_facet endosulfan
glutathione
glutathione transferase
heparin
superoxide dismutase
acrosome
animal experiment
Article
chromatin
controlled study
DNA fragmentation
enzyme activity
epididymis
in vitro study
lipid peroxidation
male
mammal
mouse
nonhuman
oxidative stress
priority journal
seminal plasma
spermatozoon
spermatozoon motility
spermatozoon penetration
transmission electron microscopy
TUNEL assay
description Endosulfan is an organochloride insecticide extensively used in several countries to protect crops from pests. As several studies indicate that endosulfan can affect human and animal development, the aim of this study was to analyse whether sperm parameters and the process of chromatin decondensation could be altered by endosulfan in mice sperm. Spermatozoa from cauda epididymis were obtained from mature male mice and incubated in the presence of two commercial formulations (CFs) of endosulfan (Master® and Zebra Ciagro®) or the active ingredient (AI) alone. A significant decrease in the percentage motility and viability of spermatozoa with respect to controls was found. In vitro decondensation was performed in the presence of glutathione and heparin. Spermatozoa incubated with the AI, endosulfan Master® and endosulfan Zebra Ciagro® showed an increase in chromatin decondensation. In addition, the TUNEL assay showed that DNA fragmentation was significantly higher when sperm were incubated with either one of the CFs when compared to the AI or controls. The ultrastructure analysis of sperm cells showed evident changes in the structure of the plasma and acrosome membranes of sperm incubated with endosulfan AI or the CFs. These results suggest that endosulfan can affect sperm integrity and in vitro chromatin decondensation as well as DNA fragmentation. © 2017 The Royal Society of Chemistry.
format JOUR
author Sánchez, M.C.
Alvarez Sedó, C.
Chaufan, G.R.
Romanato, M.
Da Cuña, R.
Lo Nostro, F.
Calvo, J.C.
Fontana, V.
author_facet Sánchez, M.C.
Alvarez Sedó, C.
Chaufan, G.R.
Romanato, M.
Da Cuña, R.
Lo Nostro, F.
Calvo, J.C.
Fontana, V.
author_sort Sánchez, M.C.
title In vitro effects of endosulfan-based insecticides on mammalian sperm
title_short In vitro effects of endosulfan-based insecticides on mammalian sperm
title_full In vitro effects of endosulfan-based insecticides on mammalian sperm
title_fullStr In vitro effects of endosulfan-based insecticides on mammalian sperm
title_full_unstemmed In vitro effects of endosulfan-based insecticides on mammalian sperm
title_sort in vitro effects of endosulfan-based insecticides on mammalian sperm
url http://hdl.handle.net/20.500.12110/paper_2045452X_v7_n1_p117_Sanchez
work_keys_str_mv AT sanchezmc invitroeffectsofendosulfanbasedinsecticidesonmammaliansperm
AT alvarezsedoc invitroeffectsofendosulfanbasedinsecticidesonmammaliansperm
AT chaufangr invitroeffectsofendosulfanbasedinsecticidesonmammaliansperm
AT romanatom invitroeffectsofendosulfanbasedinsecticidesonmammaliansperm
AT dacunar invitroeffectsofendosulfanbasedinsecticidesonmammaliansperm
AT lonostrof invitroeffectsofendosulfanbasedinsecticidesonmammaliansperm
AT calvojc invitroeffectsofendosulfanbasedinsecticidesonmammaliansperm
AT fontanav invitroeffectsofendosulfanbasedinsecticidesonmammaliansperm
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