Role of "well known" proteins on cell wall degradation and softening

Fruit softening has been largely associated with cell wall degradation by a number of loosening proteins. Firmness is a major quality attribute of fresh cherries and also an important factor affecting the susceptibility to postharvest rots. By analyzing the solubilization, depolymerization and compo...

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Detalles Bibliográficos
Autores principales: Vicente, A.R., Rodoni, L.M., Salato, G.S., Basanta, M.F., Ponce, N.M.A., Stortz, C.A., Raffo, M.D., Powell, A.L.T., Bennett, A.B., Labavitch, J.M., Toivonen P.M., Kalaitzis P., Manganaris G.A.
Formato: SER
Materias:
Cell wall
Cherry
Expansin
Polygalacturonase
Softening
Tomato
Lycopersicon esculentum
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_05677572_v1079_n_p61_Vicente
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_05677572_v1079_n_p61_Vicente
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spelling todo:paper_05677572_v1079_n_p61_Vicente2023-10-03T15:35:53Z Role of "well known" proteins on cell wall degradation and softening Vicente, A.R. Rodoni, L.M. Salato, G.S. Basanta, M.F. Ponce, N.M.A. Stortz, C.A. Raffo, M.D. Powell, A.L.T. Bennett, A.B. Labavitch, J.M. Toivonen P.M. Kalaitzis P. Manganaris G.A. Cell wall Cherry Expansin Polygalacturonase Softening Tomato Lycopersicon esculentum Fruit softening has been largely associated with cell wall degradation by a number of loosening proteins. Firmness is a major quality attribute of fresh cherries and also an important factor affecting the susceptibility to postharvest rots. By analyzing the solubilization, depolymerization and composition of pectins and hemicelluloses in cultivars with contrasting firmness we found that the pattern and extent of their wall disassembly was quite similar. No marked pectin or hemicellulose depolymerization was observed and a similar reduction in tightlybound pectins and hemicellulose was detected in both cultivars during ripening. However, firm cherries presented pectic polymers with lower proportion of neutral sugars compared to uronic acids, suggesting that the variation in total wall polysaccharide and the branching of pectins assembled early in development or the proportion of homogalacturonan (HG) to type rhamnogalacturonan-I (RG-I) may contribute to the differences in firmness between cultivars. Dismantling of the cell wall by the action of relatively "well known" loosening agents is involved in the progressive softening occurring during ripening. Two of these proteins include polygalacturonases (PG) which are known to hydrolyze homogalacturonans and expansins (Exp) believed to participate in the relaxation of the cell wall by reducing hydrogen bonding between cellulose microfibrils and xyloglucan. We investigated the in vivo roles of these wall-disassembling proteins, by overexpressing PG and Exp1 both alone and in combination in a non-ripening rin tomato background. The simultaneous overexpression of PG and Exp1 in rin fruit restored softening in these non-ripening fruit. Unexpectedly, PG overexpression resulted in higher hemicellulose depolymerization while increased levels of Exp1 accelerated pectin turnover. This shows that besides their "well known" in vitro functions these proteins act in muro by facilitating the degradation of non-directly targeted wall components, likely by increasing the accessibility of pre-existing wall-degrading proteins to their polysaccharide substrates. Fil:Basanta, M.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Stortz, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. SER info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_05677572_v1079_n_p61_Vicente
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Cell wall
Cherry
Expansin
Polygalacturonase
Softening
Tomato
Lycopersicon esculentum
spellingShingle Cell wall
Cherry
Expansin
Polygalacturonase
Softening
Tomato
Lycopersicon esculentum
Vicente, A.R.
Rodoni, L.M.
Salato, G.S.
Basanta, M.F.
Ponce, N.M.A.
Stortz, C.A.
Raffo, M.D.
Powell, A.L.T.
Bennett, A.B.
Labavitch, J.M.
Toivonen P.M.
Kalaitzis P.
Manganaris G.A.
Role of "well known" proteins on cell wall degradation and softening
topic_facet Cell wall
Cherry
Expansin
Polygalacturonase
Softening
Tomato
Lycopersicon esculentum
description Fruit softening has been largely associated with cell wall degradation by a number of loosening proteins. Firmness is a major quality attribute of fresh cherries and also an important factor affecting the susceptibility to postharvest rots. By analyzing the solubilization, depolymerization and composition of pectins and hemicelluloses in cultivars with contrasting firmness we found that the pattern and extent of their wall disassembly was quite similar. No marked pectin or hemicellulose depolymerization was observed and a similar reduction in tightlybound pectins and hemicellulose was detected in both cultivars during ripening. However, firm cherries presented pectic polymers with lower proportion of neutral sugars compared to uronic acids, suggesting that the variation in total wall polysaccharide and the branching of pectins assembled early in development or the proportion of homogalacturonan (HG) to type rhamnogalacturonan-I (RG-I) may contribute to the differences in firmness between cultivars. Dismantling of the cell wall by the action of relatively "well known" loosening agents is involved in the progressive softening occurring during ripening. Two of these proteins include polygalacturonases (PG) which are known to hydrolyze homogalacturonans and expansins (Exp) believed to participate in the relaxation of the cell wall by reducing hydrogen bonding between cellulose microfibrils and xyloglucan. We investigated the in vivo roles of these wall-disassembling proteins, by overexpressing PG and Exp1 both alone and in combination in a non-ripening rin tomato background. The simultaneous overexpression of PG and Exp1 in rin fruit restored softening in these non-ripening fruit. Unexpectedly, PG overexpression resulted in higher hemicellulose depolymerization while increased levels of Exp1 accelerated pectin turnover. This shows that besides their "well known" in vitro functions these proteins act in muro by facilitating the degradation of non-directly targeted wall components, likely by increasing the accessibility of pre-existing wall-degrading proteins to their polysaccharide substrates.
format SER
author Vicente, A.R.
Rodoni, L.M.
Salato, G.S.
Basanta, M.F.
Ponce, N.M.A.
Stortz, C.A.
Raffo, M.D.
Powell, A.L.T.
Bennett, A.B.
Labavitch, J.M.
Toivonen P.M.
Kalaitzis P.
Manganaris G.A.
author_facet Vicente, A.R.
Rodoni, L.M.
Salato, G.S.
Basanta, M.F.
Ponce, N.M.A.
Stortz, C.A.
Raffo, M.D.
Powell, A.L.T.
Bennett, A.B.
Labavitch, J.M.
Toivonen P.M.
Kalaitzis P.
Manganaris G.A.
author_sort Vicente, A.R.
title Role of "well known" proteins on cell wall degradation and softening
title_short Role of "well known" proteins on cell wall degradation and softening
title_full Role of "well known" proteins on cell wall degradation and softening
title_fullStr Role of "well known" proteins on cell wall degradation and softening
title_full_unstemmed Role of "well known" proteins on cell wall degradation and softening
title_sort role of "well known" proteins on cell wall degradation and softening
url http://hdl.handle.net/20.500.12110/paper_05677572_v1079_n_p61_Vicente
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