Combined effect of water activity and pH on the inhibition of Escherichia coli by nisin

The Doehlert design and surface response methodology were used to study the influence of pH and water activity (aw) on Escherichia coli inhibition by nisin. Combining stress factors at levels where they are not inhibitory by themselves, a reduction of E. coli survival fraction can be achieved with l...

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Autores principales: Cerrutti, P., Terebiznik, M.R., De Huergo, M.S., Jagus, R., Pilosof, A.M.R.
Formato: JOUR
Materias:
bacterial cell
bacterial count
bacterial growth
cell survival
colony forming unit
food safety
growth inhibition
nisin
pH
water flow
Bacteria (microorganisms)
Escherichia coli
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0362028X_v64_n10_p1510_Cerrutti
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_0362028X_v64_n10_p1510_Cerrutti
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spelling todo:paper_0362028X_v64_n10_p1510_Cerrutti2023-10-03T15:26:48Z Combined effect of water activity and pH on the inhibition of Escherichia coli by nisin Cerrutti, P. Terebiznik, M.R. De Huergo, M.S. Jagus, R. Pilosof, A.M.R. bacterial cell bacterial count bacterial growth cell survival colony forming unit food safety growth inhibition nisin pH water flow Bacteria (microorganisms) Escherichia coli Escherichia coli The Doehlert design and surface response methodology were used to study the influence of pH and water activity (aw) on Escherichia coli inhibition by nisin. Combining stress factors at levels where they are not inhibitory by themselves, a reduction of E. coli survival fraction can be achieved with lower nisin doses than in a single nisin treatment. For all the pH values assayed, a synergistic effect of aw and nisin concentration was detected, and the isoresponse lines showed the existence of an area of maximum inhibition. Factors that reduced viable cell counts by 4 to 5 log cycles were 1,000 to 1,400 IU of nisin per ml at pH 5.5 to 6.5 and a water activity of 0.97 and 0,98. The addition of different ionic and nonionic solutes to control aw suggested that the effect of aw in the inhibitory action of nisin on E. coli cells was not solute-specific. The use of the Doehlert experimental design was effective to determine the optimal combination of stress factors, as well as to point out the most important variables that affected E. coli inhibition. Fil:Cerrutti, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Terebiznik, M.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Jagus, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pilosof, A.M.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0362028X_v64_n10_p1510_Cerrutti
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic bacterial cell
bacterial count
bacterial growth
cell survival
colony forming unit
food safety
growth inhibition
nisin
pH
water flow
Bacteria (microorganisms)
Escherichia coli
Escherichia coli
spellingShingle bacterial cell
bacterial count
bacterial growth
cell survival
colony forming unit
food safety
growth inhibition
nisin
pH
water flow
Bacteria (microorganisms)
Escherichia coli
Escherichia coli
Cerrutti, P.
Terebiznik, M.R.
De Huergo, M.S.
Jagus, R.
Pilosof, A.M.R.
Combined effect of water activity and pH on the inhibition of Escherichia coli by nisin
topic_facet bacterial cell
bacterial count
bacterial growth
cell survival
colony forming unit
food safety
growth inhibition
nisin
pH
water flow
Bacteria (microorganisms)
Escherichia coli
Escherichia coli
description The Doehlert design and surface response methodology were used to study the influence of pH and water activity (aw) on Escherichia coli inhibition by nisin. Combining stress factors at levels where they are not inhibitory by themselves, a reduction of E. coli survival fraction can be achieved with lower nisin doses than in a single nisin treatment. For all the pH values assayed, a synergistic effect of aw and nisin concentration was detected, and the isoresponse lines showed the existence of an area of maximum inhibition. Factors that reduced viable cell counts by 4 to 5 log cycles were 1,000 to 1,400 IU of nisin per ml at pH 5.5 to 6.5 and a water activity of 0.97 and 0,98. The addition of different ionic and nonionic solutes to control aw suggested that the effect of aw in the inhibitory action of nisin on E. coli cells was not solute-specific. The use of the Doehlert experimental design was effective to determine the optimal combination of stress factors, as well as to point out the most important variables that affected E. coli inhibition.
format JOUR
author Cerrutti, P.
Terebiznik, M.R.
De Huergo, M.S.
Jagus, R.
Pilosof, A.M.R.
author_facet Cerrutti, P.
Terebiznik, M.R.
De Huergo, M.S.
Jagus, R.
Pilosof, A.M.R.
author_sort Cerrutti, P.
title Combined effect of water activity and pH on the inhibition of Escherichia coli by nisin
title_short Combined effect of water activity and pH on the inhibition of Escherichia coli by nisin
title_full Combined effect of water activity and pH on the inhibition of Escherichia coli by nisin
title_fullStr Combined effect of water activity and pH on the inhibition of Escherichia coli by nisin
title_full_unstemmed Combined effect of water activity and pH on the inhibition of Escherichia coli by nisin
title_sort combined effect of water activity and ph on the inhibition of escherichia coli by nisin
url http://hdl.handle.net/20.500.12110/paper_0362028X_v64_n10_p1510_Cerrutti
work_keys_str_mv AT cerruttip combinedeffectofwateractivityandphontheinhibitionofescherichiacolibynisin
AT terebiznikmr combinedeffectofwateractivityandphontheinhibitionofescherichiacolibynisin
AT dehuergoms combinedeffectofwateractivityandphontheinhibitionofescherichiacolibynisin
AT jagusr combinedeffectofwateractivityandphontheinhibitionofescherichiacolibynisin
AT pilosofamr combinedeffectofwateractivityandphontheinhibitionofescherichiacolibynisin
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