Galectin-3 negatively regulates the frequency and function of CD4 + CD25 + Foxp3 + regulatory T cells and influences the course of Leishmania major infection
Galectin-3, an endogenous glycan-binding protein, plays essential roles during microbial infection by modulating innate and adaptive immunity. However, the role of galectin-3 within the CD4 + CD25 + Foxp3 + T regulatory (T REG ) cell compartment has not yet been explored. Here, we found, in a model...
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| Autores principales: | , , , , , , , , , |
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| Formato: | JOUR |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00142980_v43_n7_p1806_Fermino |
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| Sumario: | Galectin-3, an endogenous glycan-binding protein, plays essential roles during microbial infection by modulating innate and adaptive immunity. However, the role of galectin-3 within the CD4 + CD25 + Foxp3 + T regulatory (T REG ) cell compartment has not yet been explored. Here, we found, in a model of Leishmania major infection, that galectin-3 deficiency increases the frequency of peripheral T REG cells both in draining lymph nodes (LNs) and sites of infection. These observations correlated with an increased severity of the disease, as shown by increased footpad swelling and parasite burden. Galectin-3-deficient (Lgals3 -/- ) T REG cells displayed higher CD103 expression, showed greater suppressive capacity, and synthesized higher amounts of IL-10 compared with their wild-type (WT) counterpart. Furthermore, both T REG cells and T effector (T EFF ) cells from Lgals3 -/- mice showed higher expression of Notch1 and the Notch target gene Hes-1. Interestingly, Notch signaling components were also altered in both T REG and T EFF cells from uninfected Lgals3 -/- mice. Thus, endogenous galectin-3 regulates the frequency and function of CD4 + CD25 + Foxp3 + T REG cells and alters the course of L. major infection. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
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