Galectin-3 negatively regulates the frequency and function of CD4 + CD25 + Foxp3 + regulatory T cells and influences the course of Leishmania major infection
Galectin-3, an endogenous glycan-binding protein, plays essential roles during microbial infection by modulating innate and adaptive immunity. However, the role of galectin-3 within the CD4 + CD25 + Foxp3 + T regulatory (T REG ) cell compartment has not yet been explored. Here, we found, in a model...
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todo:paper_00142980_v43_n7_p1806_Fermino2023-10-03T14:12:00Z Galectin-3 negatively regulates the frequency and function of CD4 + CD25 + Foxp3 + regulatory T cells and influences the course of Leishmania major infection Fermino, M.L. Dias, F.C. Lopes, C.D. Souza, M.A. Cruz, A.K. Liu, F.-T. Chammas, R. Roque-Barreira, M.C. Rabinovich, G.A. Bernardes, E.S. Galectin-3 IL-10 Leishmania major Notch signaling T regulatory (Treg) cells CD103 antigen galectin 3 gamma interferon interleukin 10 interleukin 4 Notch1 receptor transcription factor FOXP3 transcription factor HES 1 animal experiment animal model animal tissue antigen expression article CD4+ CD25+ T lymphocyte controlled study disease course disease severity effector cell flow cytometry immunohistochemistry in vitro study Leishmania major leishmaniasis lymph node lymphocyte function mouse negative feedback nonhuman parasite load priority journal regulatory T lymphocyte wild type Galectin-3 IL-10 Leishmania major Notch signaling T regulatory (Treg) cells Animals Disease Models, Animal Flow Cytometry Forkhead Transcription Factors Galectin 3 Immunohistochemistry Leishmania major Leishmaniasis, Cutaneous Mice Mice, Inbred BALB C Mice, Knockout Real-Time Polymerase Chain Reaction Receptors, Notch Reverse Transcriptase Polymerase Chain Reaction T-Lymphocytes, Regulatory Galectin-3, an endogenous glycan-binding protein, plays essential roles during microbial infection by modulating innate and adaptive immunity. However, the role of galectin-3 within the CD4 + CD25 + Foxp3 + T regulatory (T REG ) cell compartment has not yet been explored. Here, we found, in a model of Leishmania major infection, that galectin-3 deficiency increases the frequency of peripheral T REG cells both in draining lymph nodes (LNs) and sites of infection. These observations correlated with an increased severity of the disease, as shown by increased footpad swelling and parasite burden. Galectin-3-deficient (Lgals3 -/- ) T REG cells displayed higher CD103 expression, showed greater suppressive capacity, and synthesized higher amounts of IL-10 compared with their wild-type (WT) counterpart. Furthermore, both T REG cells and T effector (T EFF ) cells from Lgals3 -/- mice showed higher expression of Notch1 and the Notch target gene Hes-1. Interestingly, Notch signaling components were also altered in both T REG and T EFF cells from uninfected Lgals3 -/- mice. Thus, endogenous galectin-3 regulates the frequency and function of CD4 + CD25 + Foxp3 + T REG cells and alters the course of L. major infection. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00142980_v43_n7_p1806_Fermino |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Galectin-3 IL-10 Leishmania major Notch signaling T regulatory (Treg) cells CD103 antigen galectin 3 gamma interferon interleukin 10 interleukin 4 Notch1 receptor transcription factor FOXP3 transcription factor HES 1 animal experiment animal model animal tissue antigen expression article CD4+ CD25+ T lymphocyte controlled study disease course disease severity effector cell flow cytometry immunohistochemistry in vitro study Leishmania major leishmaniasis lymph node lymphocyte function mouse negative feedback nonhuman parasite load priority journal regulatory T lymphocyte wild type Galectin-3 IL-10 Leishmania major Notch signaling T regulatory (Treg) cells Animals Disease Models, Animal Flow Cytometry Forkhead Transcription Factors Galectin 3 Immunohistochemistry Leishmania major Leishmaniasis, Cutaneous Mice Mice, Inbred BALB C Mice, Knockout Real-Time Polymerase Chain Reaction Receptors, Notch Reverse Transcriptase Polymerase Chain Reaction T-Lymphocytes, Regulatory |
| spellingShingle |
Galectin-3 IL-10 Leishmania major Notch signaling T regulatory (Treg) cells CD103 antigen galectin 3 gamma interferon interleukin 10 interleukin 4 Notch1 receptor transcription factor FOXP3 transcription factor HES 1 animal experiment animal model animal tissue antigen expression article CD4+ CD25+ T lymphocyte controlled study disease course disease severity effector cell flow cytometry immunohistochemistry in vitro study Leishmania major leishmaniasis lymph node lymphocyte function mouse negative feedback nonhuman parasite load priority journal regulatory T lymphocyte wild type Galectin-3 IL-10 Leishmania major Notch signaling T regulatory (Treg) cells Animals Disease Models, Animal Flow Cytometry Forkhead Transcription Factors Galectin 3 Immunohistochemistry Leishmania major Leishmaniasis, Cutaneous Mice Mice, Inbred BALB C Mice, Knockout Real-Time Polymerase Chain Reaction Receptors, Notch Reverse Transcriptase Polymerase Chain Reaction T-Lymphocytes, Regulatory Fermino, M.L. Dias, F.C. Lopes, C.D. Souza, M.A. Cruz, A.K. Liu, F.-T. Chammas, R. Roque-Barreira, M.C. Rabinovich, G.A. Bernardes, E.S. Galectin-3 negatively regulates the frequency and function of CD4 + CD25 + Foxp3 + regulatory T cells and influences the course of Leishmania major infection |
| topic_facet |
Galectin-3 IL-10 Leishmania major Notch signaling T regulatory (Treg) cells CD103 antigen galectin 3 gamma interferon interleukin 10 interleukin 4 Notch1 receptor transcription factor FOXP3 transcription factor HES 1 animal experiment animal model animal tissue antigen expression article CD4+ CD25+ T lymphocyte controlled study disease course disease severity effector cell flow cytometry immunohistochemistry in vitro study Leishmania major leishmaniasis lymph node lymphocyte function mouse negative feedback nonhuman parasite load priority journal regulatory T lymphocyte wild type Galectin-3 IL-10 Leishmania major Notch signaling T regulatory (Treg) cells Animals Disease Models, Animal Flow Cytometry Forkhead Transcription Factors Galectin 3 Immunohistochemistry Leishmania major Leishmaniasis, Cutaneous Mice Mice, Inbred BALB C Mice, Knockout Real-Time Polymerase Chain Reaction Receptors, Notch Reverse Transcriptase Polymerase Chain Reaction T-Lymphocytes, Regulatory |
| description |
Galectin-3, an endogenous glycan-binding protein, plays essential roles during microbial infection by modulating innate and adaptive immunity. However, the role of galectin-3 within the CD4 + CD25 + Foxp3 + T regulatory (T REG ) cell compartment has not yet been explored. Here, we found, in a model of Leishmania major infection, that galectin-3 deficiency increases the frequency of peripheral T REG cells both in draining lymph nodes (LNs) and sites of infection. These observations correlated with an increased severity of the disease, as shown by increased footpad swelling and parasite burden. Galectin-3-deficient (Lgals3 -/- ) T REG cells displayed higher CD103 expression, showed greater suppressive capacity, and synthesized higher amounts of IL-10 compared with their wild-type (WT) counterpart. Furthermore, both T REG cells and T effector (T EFF ) cells from Lgals3 -/- mice showed higher expression of Notch1 and the Notch target gene Hes-1. Interestingly, Notch signaling components were also altered in both T REG and T EFF cells from uninfected Lgals3 -/- mice. Thus, endogenous galectin-3 regulates the frequency and function of CD4 + CD25 + Foxp3 + T REG cells and alters the course of L. major infection. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
| format |
JOUR |
| author |
Fermino, M.L. Dias, F.C. Lopes, C.D. Souza, M.A. Cruz, A.K. Liu, F.-T. Chammas, R. Roque-Barreira, M.C. Rabinovich, G.A. Bernardes, E.S. |
| author_facet |
Fermino, M.L. Dias, F.C. Lopes, C.D. Souza, M.A. Cruz, A.K. Liu, F.-T. Chammas, R. Roque-Barreira, M.C. Rabinovich, G.A. Bernardes, E.S. |
| author_sort |
Fermino, M.L. |
| title |
Galectin-3 negatively regulates the frequency and function of CD4 + CD25 + Foxp3 + regulatory T cells and influences the course of Leishmania major infection |
| title_short |
Galectin-3 negatively regulates the frequency and function of CD4 + CD25 + Foxp3 + regulatory T cells and influences the course of Leishmania major infection |
| title_full |
Galectin-3 negatively regulates the frequency and function of CD4 + CD25 + Foxp3 + regulatory T cells and influences the course of Leishmania major infection |
| title_fullStr |
Galectin-3 negatively regulates the frequency and function of CD4 + CD25 + Foxp3 + regulatory T cells and influences the course of Leishmania major infection |
| title_full_unstemmed |
Galectin-3 negatively regulates the frequency and function of CD4 + CD25 + Foxp3 + regulatory T cells and influences the course of Leishmania major infection |
| title_sort |
galectin-3 negatively regulates the frequency and function of cd4 + cd25 + foxp3 + regulatory t cells and influences the course of leishmania major infection |
| url |
http://hdl.handle.net/20.500.12110/paper_00142980_v43_n7_p1806_Fermino |
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