The biosynthesis of a N,N′-diacetylchitobiose containing lipid by liver microsomes. A probable dolichol pyrophosphate derivative
Incubation of liver microsomes with dolichol monophosphate, Mg++ and UDP-[14C] -N-acetylglucosamine leads to the appearance of radioactivity in the lipid fraction. Mild acid treatment results in the formation of N-acetylglucosamine and N,N′-diacetylchitobiose (2-acetamido-2-deoxy-0-β-D-glucopyranosy...
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| Autores principales: | , , , |
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| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_0006291X_v52_n4_p1285_Leloir |
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| Sumario: | Incubation of liver microsomes with dolichol monophosphate, Mg++ and UDP-[14C] -N-acetylglucosamine leads to the appearance of radioactivity in the lipid fraction. Mild acid treatment results in the formation of N-acetylglucosamine and N,N′-diacetylchitobiose (2-acetamido-2-deoxy-0-β-D-glucopyranosyl-(1rarr4)-2-acetamido-2-deoxy-D-glucose). The formation of the disaccharide containing lipid was increased by incubation with crude liver lipids or by reincubation with unlabelled UDP-N-acetylglucosamine. The labelling in the latter compound varied according to whether one of the N-acetylglucosamyl residues arose from a crude lipid or from unlabelled UDP-N-acetylglucosamine. Evidence is presented indicating that the compounds are dolichol pyrophosphate derivatives. © 1973. |
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