The biosynthesis of a N,N′-diacetylchitobiose containing lipid by liver microsomes. A probable dolichol pyrophosphate derivative

Incubation of liver microsomes with dolichol monophosphate, Mg++ and UDP-[14C] -N-acetylglucosamine leads to the appearance of radioactivity in the lipid fraction. Mild acid treatment results in the formation of N-acetylglucosamine and N,N′-diacetylchitobiose (2-acetamido-2-deoxy-0-β-D-glucopyranosy...

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Autores principales:	Leloir, L.F., Staneloni, R.J., Carminatti, H., Behrens, N.H.
Formato:	JOUR
Materias:	acetic acid derivative borane derivative carbon disaccharide fatty alcohol glucosamine lipid magnesium terpene uridine diphosphate sugar animal article biosynthesis drug effect hydrolysis ion exchange chromatography liver microsome metabolism oxidation reduction reaction paper chromatography paper electrophoresis rat Acetic Acids Animal Borohydrides Carbon Isotopes Chromatography, DEAE-Cellulose Chromatography, Paper Disaccharides Electrophoresis, Paper Fatty Alcohols Glucosamine Hydrolysis Lipids Magnesium Microsomes, Liver Oxidation-Reduction Rats Terpenes Uridine Diphosphate Sugars
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_0006291X_v52_n4_p1285_Leloir
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_0006291X_v52_n4_p1285_Leloir
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spelling	todo:paper_0006291X_v52_n4_p1285_Leloir2023-10-03T14:04:06Z The biosynthesis of a N,N′-diacetylchitobiose containing lipid by liver microsomes. A probable dolichol pyrophosphate derivative Leloir, L.F. Staneloni, R.J. Carminatti, H. Behrens, N.H. acetic acid derivative borane derivative carbon disaccharide fatty alcohol glucosamine lipid magnesium terpene uridine diphosphate sugar animal article biosynthesis drug effect hydrolysis ion exchange chromatography liver microsome metabolism oxidation reduction reaction paper chromatography paper electrophoresis rat Acetic Acids Animal Borohydrides Carbon Isotopes Chromatography, DEAE-Cellulose Chromatography, Paper Disaccharides Electrophoresis, Paper Fatty Alcohols Glucosamine Hydrolysis Lipids Magnesium Microsomes, Liver Oxidation-Reduction Rats Terpenes Uridine Diphosphate Sugars Incubation of liver microsomes with dolichol monophosphate, Mg++ and UDP-[14C] -N-acetylglucosamine leads to the appearance of radioactivity in the lipid fraction. Mild acid treatment results in the formation of N-acetylglucosamine and N,N′-diacetylchitobiose (2-acetamido-2-deoxy-0-β-D-glucopyranosyl-(1rarr4)-2-acetamido-2-deoxy-D-glucose). The formation of the disaccharide containing lipid was increased by incubation with crude liver lipids or by reincubation with unlabelled UDP-N-acetylglucosamine. The labelling in the latter compound varied according to whether one of the N-acetylglucosamyl residues arose from a crude lipid or from unlabelled UDP-N-acetylglucosamine. Evidence is presented indicating that the compounds are dolichol pyrophosphate derivatives. © 1973. Fil:Carminatti, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Behrens, N.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0006291X_v52_n4_p1285_Leloir
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	acetic acid derivative borane derivative carbon disaccharide fatty alcohol glucosamine lipid magnesium terpene uridine diphosphate sugar animal article biosynthesis drug effect hydrolysis ion exchange chromatography liver microsome metabolism oxidation reduction reaction paper chromatography paper electrophoresis rat Acetic Acids Animal Borohydrides Carbon Isotopes Chromatography, DEAE-Cellulose Chromatography, Paper Disaccharides Electrophoresis, Paper Fatty Alcohols Glucosamine Hydrolysis Lipids Magnesium Microsomes, Liver Oxidation-Reduction Rats Terpenes Uridine Diphosphate Sugars
spellingShingle	acetic acid derivative borane derivative carbon disaccharide fatty alcohol glucosamine lipid magnesium terpene uridine diphosphate sugar animal article biosynthesis drug effect hydrolysis ion exchange chromatography liver microsome metabolism oxidation reduction reaction paper chromatography paper electrophoresis rat Acetic Acids Animal Borohydrides Carbon Isotopes Chromatography, DEAE-Cellulose Chromatography, Paper Disaccharides Electrophoresis, Paper Fatty Alcohols Glucosamine Hydrolysis Lipids Magnesium Microsomes, Liver Oxidation-Reduction Rats Terpenes Uridine Diphosphate Sugars Leloir, L.F. Staneloni, R.J. Carminatti, H. Behrens, N.H. The biosynthesis of a N,N′-diacetylchitobiose containing lipid by liver microsomes. A probable dolichol pyrophosphate derivative
topic_facet	acetic acid derivative borane derivative carbon disaccharide fatty alcohol glucosamine lipid magnesium terpene uridine diphosphate sugar animal article biosynthesis drug effect hydrolysis ion exchange chromatography liver microsome metabolism oxidation reduction reaction paper chromatography paper electrophoresis rat Acetic Acids Animal Borohydrides Carbon Isotopes Chromatography, DEAE-Cellulose Chromatography, Paper Disaccharides Electrophoresis, Paper Fatty Alcohols Glucosamine Hydrolysis Lipids Magnesium Microsomes, Liver Oxidation-Reduction Rats Terpenes Uridine Diphosphate Sugars
description	Incubation of liver microsomes with dolichol monophosphate, Mg++ and UDP-[14C] -N-acetylglucosamine leads to the appearance of radioactivity in the lipid fraction. Mild acid treatment results in the formation of N-acetylglucosamine and N,N′-diacetylchitobiose (2-acetamido-2-deoxy-0-β-D-glucopyranosyl-(1rarr4)-2-acetamido-2-deoxy-D-glucose). The formation of the disaccharide containing lipid was increased by incubation with crude liver lipids or by reincubation with unlabelled UDP-N-acetylglucosamine. The labelling in the latter compound varied according to whether one of the N-acetylglucosamyl residues arose from a crude lipid or from unlabelled UDP-N-acetylglucosamine. Evidence is presented indicating that the compounds are dolichol pyrophosphate derivatives. © 1973.
format	JOUR
author	Leloir, L.F. Staneloni, R.J. Carminatti, H. Behrens, N.H.
author_facet	Leloir, L.F. Staneloni, R.J. Carminatti, H. Behrens, N.H.
author_sort	Leloir, L.F.
title	The biosynthesis of a N,N′-diacetylchitobiose containing lipid by liver microsomes. A probable dolichol pyrophosphate derivative
title_short	The biosynthesis of a N,N′-diacetylchitobiose containing lipid by liver microsomes. A probable dolichol pyrophosphate derivative
title_full	The biosynthesis of a N,N′-diacetylchitobiose containing lipid by liver microsomes. A probable dolichol pyrophosphate derivative
title_fullStr	The biosynthesis of a N,N′-diacetylchitobiose containing lipid by liver microsomes. A probable dolichol pyrophosphate derivative
title_full_unstemmed	The biosynthesis of a N,N′-diacetylchitobiose containing lipid by liver microsomes. A probable dolichol pyrophosphate derivative
title_sort	biosynthesis of a n,n′-diacetylchitobiose containing lipid by liver microsomes. a probable dolichol pyrophosphate derivative
url	http://hdl.handle.net/20.500.12110/paper_0006291X_v52_n4_p1285_Leloir
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The biosynthesis of a N,N′-diacetylchitobiose containing lipid by liver microsomes. A probable dolichol pyrophosphate derivative

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