A 5′ RNA element promotes dengue virus RNA synthesis on a circular genome

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The mechanisms of RNA replication of plus-strand RNA viruses are still unclear. Here, we identified the first promoter element for RNA synthesis described in a flavivirus. Using dengue virus as a model, we found that the viral RdRp discriminates the viral RNA by specific recognition of a 5′ element...

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Autores principales:	Filomatori, C.V., Lodeiro, M.F., Alvarez, D.E., Samsa, M.M., Pietrasanta, L., Gamarnik, A.V.
Formato:	Artículo publishedVersion
Lenguaje:	Inglés
Publicado:	2006
Materias:	AFM Flavivirus RNA cyclization RNA-dependent RNA polymerase Viral RNA synthesis article controlled study Dengue virus genome nonhuman priority journal RNA replication RNA synthesis RNA virus atomic force microscopy biosynthesis chemistry conformation genetics metabolism physiology regulatory RNA sequence virus genome virus replication RNA viruses NS5 protein, flavivirus RNA directed RNA polymerase virus protein virus RNA Dengue Virus Genome, Viral Microscopy, Atomic Force Nucleic Acid Conformation Regulatory Sequences, Ribonucleic Acid RNA Replicase RNA, Viral Viral Nonstructural Proteins Virus Replication
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_08909369_v20_n16_p2238_Filomatori
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paperaa:paper_08909369_v20_n16_p2238_Filomatori2023-06-12T16:48:23Z A 5′ RNA element promotes dengue virus RNA synthesis on a circular genome Genes Dev. 2006;20(16):2238-2249 Filomatori, C.V. Lodeiro, M.F. Alvarez, D.E. Samsa, M.M. Pietrasanta, L. Gamarnik, A.V. AFM Flavivirus RNA cyclization RNA-dependent RNA polymerase Viral RNA synthesis article controlled study Dengue virus genome nonhuman priority journal RNA replication RNA synthesis RNA virus atomic force microscopy biosynthesis chemistry conformation Dengue virus genetics metabolism physiology regulatory RNA sequence virus genome virus replication Dengue virus Flavivirus RNA viruses NS5 protein, flavivirus RNA directed RNA polymerase virus protein virus RNA Dengue Virus Genome, Viral Microscopy, Atomic Force Nucleic Acid Conformation Regulatory Sequences, Ribonucleic Acid RNA Replicase RNA, Viral Viral Nonstructural Proteins Virus Replication The mechanisms of RNA replication of plus-strand RNA viruses are still unclear. Here, we identified the first promoter element for RNA synthesis described in a flavivirus. Using dengue virus as a model, we found that the viral RdRp discriminates the viral RNA by specific recognition of a 5′ element named SLA. We demonstrated that RNA-RNA interactions between 5′ and 3′ end sequences of the viral genome enhance dengue virus RNA synthesis only in the presence of an intact SLA. We propose a novel mechanism for minus-strand RNA synthesis in which the viral polymerase binds SLA at the 5′ end of the genome and reaches the site of initiation at the 3′ end via long-range RNA-RNA interactions. These findings provide an explanation for the strict requirement of dengue virus genome cyclization during viral replication. © 2006 by Cold Spring Harbor Laboratory Press. 2006 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_08909369_v20_n16_p2238_Filomatori
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	Inglés
orig_language_str_mv	eng
topic	AFM Flavivirus RNA cyclization RNA-dependent RNA polymerase Viral RNA synthesis article controlled study Dengue virus genome nonhuman priority journal RNA replication RNA synthesis RNA virus atomic force microscopy biosynthesis chemistry conformation Dengue virus genetics metabolism physiology regulatory RNA sequence virus genome virus replication Dengue virus Flavivirus RNA viruses NS5 protein, flavivirus RNA directed RNA polymerase virus protein virus RNA Dengue Virus Genome, Viral Microscopy, Atomic Force Nucleic Acid Conformation Regulatory Sequences, Ribonucleic Acid RNA Replicase RNA, Viral Viral Nonstructural Proteins Virus Replication
spellingShingle	AFM Flavivirus RNA cyclization RNA-dependent RNA polymerase Viral RNA synthesis article controlled study Dengue virus genome nonhuman priority journal RNA replication RNA synthesis RNA virus atomic force microscopy biosynthesis chemistry conformation Dengue virus genetics metabolism physiology regulatory RNA sequence virus genome virus replication Dengue virus Flavivirus RNA viruses NS5 protein, flavivirus RNA directed RNA polymerase virus protein virus RNA Dengue Virus Genome, Viral Microscopy, Atomic Force Nucleic Acid Conformation Regulatory Sequences, Ribonucleic Acid RNA Replicase RNA, Viral Viral Nonstructural Proteins Virus Replication Filomatori, C.V. Lodeiro, M.F. Alvarez, D.E. Samsa, M.M. Pietrasanta, L. Gamarnik, A.V. A 5′ RNA element promotes dengue virus RNA synthesis on a circular genome
topic_facet	AFM Flavivirus RNA cyclization RNA-dependent RNA polymerase Viral RNA synthesis article controlled study Dengue virus genome nonhuman priority journal RNA replication RNA synthesis RNA virus atomic force microscopy biosynthesis chemistry conformation Dengue virus genetics metabolism physiology regulatory RNA sequence virus genome virus replication Dengue virus Flavivirus RNA viruses NS5 protein, flavivirus RNA directed RNA polymerase virus protein virus RNA Dengue Virus Genome, Viral Microscopy, Atomic Force Nucleic Acid Conformation Regulatory Sequences, Ribonucleic Acid RNA Replicase RNA, Viral Viral Nonstructural Proteins Virus Replication
description	The mechanisms of RNA replication of plus-strand RNA viruses are still unclear. Here, we identified the first promoter element for RNA synthesis described in a flavivirus. Using dengue virus as a model, we found that the viral RdRp discriminates the viral RNA by specific recognition of a 5′ element named SLA. We demonstrated that RNA-RNA interactions between 5′ and 3′ end sequences of the viral genome enhance dengue virus RNA synthesis only in the presence of an intact SLA. We propose a novel mechanism for minus-strand RNA synthesis in which the viral polymerase binds SLA at the 5′ end of the genome and reaches the site of initiation at the 3′ end via long-range RNA-RNA interactions. These findings provide an explanation for the strict requirement of dengue virus genome cyclization during viral replication. © 2006 by Cold Spring Harbor Laboratory Press.
format	Artículo Artículo publishedVersion
author	Filomatori, C.V. Lodeiro, M.F. Alvarez, D.E. Samsa, M.M. Pietrasanta, L. Gamarnik, A.V.
author_facet	Filomatori, C.V. Lodeiro, M.F. Alvarez, D.E. Samsa, M.M. Pietrasanta, L. Gamarnik, A.V.
author_sort	Filomatori, C.V.
title	A 5′ RNA element promotes dengue virus RNA synthesis on a circular genome
title_short	A 5′ RNA element promotes dengue virus RNA synthesis on a circular genome
title_full	A 5′ RNA element promotes dengue virus RNA synthesis on a circular genome
title_fullStr	A 5′ RNA element promotes dengue virus RNA synthesis on a circular genome
title_full_unstemmed	A 5′ RNA element promotes dengue virus RNA synthesis on a circular genome
title_sort	5′ rna element promotes dengue virus rna synthesis on a circular genome
publishDate	2006
url	http://hdl.handle.net/20.500.12110/paper_08909369_v20_n16_p2238_Filomatori
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A 5′ RNA element promotes dengue virus RNA synthesis on a circular genome

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