Hypoxia-induced cell death and activation of pro- and anti-apoptotic proteins in developing chick optic lobe
Exposure of the CNS to hypoxia is associated with cell death. Our aim was to establish a temporal correlation between cellular and molecular alterations induced by an acute hypoxia evaluated at different post-hypoxia (p-h) times and at two stages of chick optic lobe development: embryonic days (ED)...
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paper:paper_03643190_v31_n8_p1003_Vacotto2023-06-08T15:35:36Z Hypoxia-induced cell death and activation of pro- and anti-apoptotic proteins in developing chick optic lobe Vacotto, Marina Paz, Dante Agustin Apoptosis Caspase-3 Chick optic lobe CNS development Hypoxia Programmed cell death caspase 3 caspase 9 protein bcl 2 animal cell animal tissue article brain development brain hypoxia chick embryo controlled study embryo enzyme activity nerve cell necrosis nick end labeling nonhuman optic lobe prenatal exposure priority journal Animals Anoxia Apoptosis Apoptosis Regulatory Proteins Caspase 3 Caspase 9 Chick Embryo In Situ Nick-End Labeling Optic Lobe Optic Lobe, Nonmammalian Proto-Oncogene Proteins c-bcl-2 Exposure of the CNS to hypoxia is associated with cell death. Our aim was to establish a temporal correlation between cellular and molecular alterations induced by an acute hypoxia evaluated at different post-hypoxia (p-h) times and at two stages of chick optic lobe development: embryonic days (ED) 12 and 18. TUNEL assays at ED12 disclosed a significant increase (300%) in pyknotic cells at 6 h p-h, while at ED18 no morphological changes were observed in hypoxic versus controls. At ED12 there was a significant increase (48%) in Bcl-2 levels at the end of the hypoxic treatment, followed by a significant increase of active caspase-9 (49%) and active caspase-3 (58%) at 30 and 60 min p-h, respectively, while at ED18 no significant changes were observed. These findings indicate that prenatal hypoxia produces an equilibrated imbalance in both pro- and anti-apoptotic proteins that culminates in a process of cell death, present at earlier stages of development. © Springer Science+Business Media, Inc. 2006. Fil:Vacotto, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Paz, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2006 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03643190_v31_n8_p1003_Vacotto http://hdl.handle.net/20.500.12110/paper_03643190_v31_n8_p1003_Vacotto |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Apoptosis Caspase-3 Chick optic lobe CNS development Hypoxia Programmed cell death caspase 3 caspase 9 protein bcl 2 animal cell animal tissue article brain development brain hypoxia chick embryo controlled study embryo enzyme activity nerve cell necrosis nick end labeling nonhuman optic lobe prenatal exposure priority journal Animals Anoxia Apoptosis Apoptosis Regulatory Proteins Caspase 3 Caspase 9 Chick Embryo In Situ Nick-End Labeling Optic Lobe Optic Lobe, Nonmammalian Proto-Oncogene Proteins c-bcl-2 |
spellingShingle |
Apoptosis Caspase-3 Chick optic lobe CNS development Hypoxia Programmed cell death caspase 3 caspase 9 protein bcl 2 animal cell animal tissue article brain development brain hypoxia chick embryo controlled study embryo enzyme activity nerve cell necrosis nick end labeling nonhuman optic lobe prenatal exposure priority journal Animals Anoxia Apoptosis Apoptosis Regulatory Proteins Caspase 3 Caspase 9 Chick Embryo In Situ Nick-End Labeling Optic Lobe Optic Lobe, Nonmammalian Proto-Oncogene Proteins c-bcl-2 Vacotto, Marina Paz, Dante Agustin Hypoxia-induced cell death and activation of pro- and anti-apoptotic proteins in developing chick optic lobe |
topic_facet |
Apoptosis Caspase-3 Chick optic lobe CNS development Hypoxia Programmed cell death caspase 3 caspase 9 protein bcl 2 animal cell animal tissue article brain development brain hypoxia chick embryo controlled study embryo enzyme activity nerve cell necrosis nick end labeling nonhuman optic lobe prenatal exposure priority journal Animals Anoxia Apoptosis Apoptosis Regulatory Proteins Caspase 3 Caspase 9 Chick Embryo In Situ Nick-End Labeling Optic Lobe Optic Lobe, Nonmammalian Proto-Oncogene Proteins c-bcl-2 |
description |
Exposure of the CNS to hypoxia is associated with cell death. Our aim was to establish a temporal correlation between cellular and molecular alterations induced by an acute hypoxia evaluated at different post-hypoxia (p-h) times and at two stages of chick optic lobe development: embryonic days (ED) 12 and 18. TUNEL assays at ED12 disclosed a significant increase (300%) in pyknotic cells at 6 h p-h, while at ED18 no morphological changes were observed in hypoxic versus controls. At ED12 there was a significant increase (48%) in Bcl-2 levels at the end of the hypoxic treatment, followed by a significant increase of active caspase-9 (49%) and active caspase-3 (58%) at 30 and 60 min p-h, respectively, while at ED18 no significant changes were observed. These findings indicate that prenatal hypoxia produces an equilibrated imbalance in both pro- and anti-apoptotic proteins that culminates in a process of cell death, present at earlier stages of development. © Springer Science+Business Media, Inc. 2006. |
author |
Vacotto, Marina Paz, Dante Agustin |
author_facet |
Vacotto, Marina Paz, Dante Agustin |
author_sort |
Vacotto, Marina |
title |
Hypoxia-induced cell death and activation of pro- and anti-apoptotic proteins in developing chick optic lobe |
title_short |
Hypoxia-induced cell death and activation of pro- and anti-apoptotic proteins in developing chick optic lobe |
title_full |
Hypoxia-induced cell death and activation of pro- and anti-apoptotic proteins in developing chick optic lobe |
title_fullStr |
Hypoxia-induced cell death and activation of pro- and anti-apoptotic proteins in developing chick optic lobe |
title_full_unstemmed |
Hypoxia-induced cell death and activation of pro- and anti-apoptotic proteins in developing chick optic lobe |
title_sort |
hypoxia-induced cell death and activation of pro- and anti-apoptotic proteins in developing chick optic lobe |
publishDate |
2006 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03643190_v31_n8_p1003_Vacotto http://hdl.handle.net/20.500.12110/paper_03643190_v31_n8_p1003_Vacotto |
work_keys_str_mv |
AT vacottomarina hypoxiainducedcelldeathandactivationofproandantiapoptoticproteinsindevelopingchickopticlobe AT pazdanteagustin hypoxiainducedcelldeathandactivationofproandantiapoptoticproteinsindevelopingchickopticlobe |
_version_ |
1768544868286595072 |