Hypoxia-induced cell death and activation of pro- and anti-apoptotic proteins in developing chick optic lobe

Exposure of the CNS to hypoxia is associated with cell death. Our aim was to establish a temporal correlation between cellular and molecular alterations induced by an acute hypoxia evaluated at different post-hypoxia (p-h) times and at two stages of chick optic lobe development: embryonic days (ED)...

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Autores principales:	Vacotto, Marina, Paz, Dante Agustin
Publicado:	2006
Materias:	Apoptosis Caspase-3 Chick optic lobe CNS development Hypoxia Programmed cell death caspase 3 caspase 9 protein bcl 2 animal cell animal tissue article brain development brain hypoxia chick embryo controlled study embryo enzyme activity nerve cell necrosis nick end labeling nonhuman optic lobe prenatal exposure priority journal Animals Anoxia Apoptosis Regulatory Proteins Caspase 3 Caspase 9 Chick Embryo In Situ Nick-End Labeling Optic Lobe Optic Lobe, Nonmammalian Proto-Oncogene Proteins c-bcl-2
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03643190_v31_n8_p1003_Vacotto http://hdl.handle.net/20.500.12110/paper_03643190_v31_n8_p1003_Vacotto
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03643190_v31_n8_p1003_Vacotto
record_format	dspace
spelling	paper:paper_03643190_v31_n8_p1003_Vacotto2023-06-08T15:35:36Z Hypoxia-induced cell death and activation of pro- and anti-apoptotic proteins in developing chick optic lobe Vacotto, Marina Paz, Dante Agustin Apoptosis Caspase-3 Chick optic lobe CNS development Hypoxia Programmed cell death caspase 3 caspase 9 protein bcl 2 animal cell animal tissue article brain development brain hypoxia chick embryo controlled study embryo enzyme activity nerve cell necrosis nick end labeling nonhuman optic lobe prenatal exposure priority journal Animals Anoxia Apoptosis Apoptosis Regulatory Proteins Caspase 3 Caspase 9 Chick Embryo In Situ Nick-End Labeling Optic Lobe Optic Lobe, Nonmammalian Proto-Oncogene Proteins c-bcl-2 Exposure of the CNS to hypoxia is associated with cell death. Our aim was to establish a temporal correlation between cellular and molecular alterations induced by an acute hypoxia evaluated at different post-hypoxia (p-h) times and at two stages of chick optic lobe development: embryonic days (ED) 12 and 18. TUNEL assays at ED12 disclosed a significant increase (300%) in pyknotic cells at 6 h p-h, while at ED18 no morphological changes were observed in hypoxic versus controls. At ED12 there was a significant increase (48%) in Bcl-2 levels at the end of the hypoxic treatment, followed by a significant increase of active caspase-9 (49%) and active caspase-3 (58%) at 30 and 60 min p-h, respectively, while at ED18 no significant changes were observed. These findings indicate that prenatal hypoxia produces an equilibrated imbalance in both pro- and anti-apoptotic proteins that culminates in a process of cell death, present at earlier stages of development. © Springer Science+Business Media, Inc. 2006. Fil:Vacotto, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Paz, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2006 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03643190_v31_n8_p1003_Vacotto http://hdl.handle.net/20.500.12110/paper_03643190_v31_n8_p1003_Vacotto
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Apoptosis Caspase-3 Chick optic lobe CNS development Hypoxia Programmed cell death caspase 3 caspase 9 protein bcl 2 animal cell animal tissue article brain development brain hypoxia chick embryo controlled study embryo enzyme activity nerve cell necrosis nick end labeling nonhuman optic lobe prenatal exposure priority journal Animals Anoxia Apoptosis Apoptosis Regulatory Proteins Caspase 3 Caspase 9 Chick Embryo In Situ Nick-End Labeling Optic Lobe Optic Lobe, Nonmammalian Proto-Oncogene Proteins c-bcl-2
spellingShingle	Apoptosis Caspase-3 Chick optic lobe CNS development Hypoxia Programmed cell death caspase 3 caspase 9 protein bcl 2 animal cell animal tissue article brain development brain hypoxia chick embryo controlled study embryo enzyme activity nerve cell necrosis nick end labeling nonhuman optic lobe prenatal exposure priority journal Animals Anoxia Apoptosis Apoptosis Regulatory Proteins Caspase 3 Caspase 9 Chick Embryo In Situ Nick-End Labeling Optic Lobe Optic Lobe, Nonmammalian Proto-Oncogene Proteins c-bcl-2 Vacotto, Marina Paz, Dante Agustin Hypoxia-induced cell death and activation of pro- and anti-apoptotic proteins in developing chick optic lobe
topic_facet	Apoptosis Caspase-3 Chick optic lobe CNS development Hypoxia Programmed cell death caspase 3 caspase 9 protein bcl 2 animal cell animal tissue article brain development brain hypoxia chick embryo controlled study embryo enzyme activity nerve cell necrosis nick end labeling nonhuman optic lobe prenatal exposure priority journal Animals Anoxia Apoptosis Apoptosis Regulatory Proteins Caspase 3 Caspase 9 Chick Embryo In Situ Nick-End Labeling Optic Lobe Optic Lobe, Nonmammalian Proto-Oncogene Proteins c-bcl-2
description	Exposure of the CNS to hypoxia is associated with cell death. Our aim was to establish a temporal correlation between cellular and molecular alterations induced by an acute hypoxia evaluated at different post-hypoxia (p-h) times and at two stages of chick optic lobe development: embryonic days (ED) 12 and 18. TUNEL assays at ED12 disclosed a significant increase (300%) in pyknotic cells at 6 h p-h, while at ED18 no morphological changes were observed in hypoxic versus controls. At ED12 there was a significant increase (48%) in Bcl-2 levels at the end of the hypoxic treatment, followed by a significant increase of active caspase-9 (49%) and active caspase-3 (58%) at 30 and 60 min p-h, respectively, while at ED18 no significant changes were observed. These findings indicate that prenatal hypoxia produces an equilibrated imbalance in both pro- and anti-apoptotic proteins that culminates in a process of cell death, present at earlier stages of development. © Springer Science+Business Media, Inc. 2006.
author	Vacotto, Marina Paz, Dante Agustin
author_facet	Vacotto, Marina Paz, Dante Agustin
author_sort	Vacotto, Marina
title	Hypoxia-induced cell death and activation of pro- and anti-apoptotic proteins in developing chick optic lobe
title_short	Hypoxia-induced cell death and activation of pro- and anti-apoptotic proteins in developing chick optic lobe
title_full	Hypoxia-induced cell death and activation of pro- and anti-apoptotic proteins in developing chick optic lobe
title_fullStr	Hypoxia-induced cell death and activation of pro- and anti-apoptotic proteins in developing chick optic lobe
title_full_unstemmed	Hypoxia-induced cell death and activation of pro- and anti-apoptotic proteins in developing chick optic lobe
title_sort	hypoxia-induced cell death and activation of pro- and anti-apoptotic proteins in developing chick optic lobe
publishDate	2006
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03643190_v31_n8_p1003_Vacotto http://hdl.handle.net/20.500.12110/paper_03643190_v31_n8_p1003_Vacotto
work_keys_str_mv	AT vacottomarina hypoxiainducedcelldeathandactivationofproandantiapoptoticproteinsindevelopingchickopticlobe AT pazdanteagustin hypoxiainducedcelldeathandactivationofproandantiapoptoticproteinsindevelopingchickopticlobe
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Hypoxia-induced cell death and activation of pro- and anti-apoptotic proteins in developing chick optic lobe

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