Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B
Heterogeneous nuclear ribonucleoproteins A and B (hnRNPs A/B), cellular RNA-binding proteins that participate in splicing, trafficking, translation and turnover of mRNAs, have been implicated in the life cycles of several cytoplasmic RNA viruses. Here, we demonstrate that silencing of hnRNPs A1 and...
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2011
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221317_v92_n9_p2181_Maeto http://hdl.handle.net/20.500.12110/paper_00221317_v92_n9_p2181_Maeto |
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paper:paper_00221317_v92_n9_p2181_Maeto2023-06-08T14:46:34Z Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B Maeto, Cynthia A. Knott, María Elena Linero, Florencia N. Ellenberg, Paula C. Scolaro, Luis Alberto Castilla, Viviana cell protein ribonucleoprotein ribonucleoprotein A1 ribonucleoprotein A2 ribonucleoprotein B small interfering RNA unclassified drug virus nucleoprotein Argentine hemorrhagic fever article cellular distribution controlled study gene overexpression immunoprecipitation Junin virus nonhuman nucleocytoplasmic transport persistent infection priority journal protein expression protein protein interaction Vero cell virus cell interaction virus replication Active Transport, Cell Nucleus Animals Cercopithecus aethiops Heterogeneous-Nuclear Ribonucleoprotein Group A-B Host-Pathogen Interactions Immunoprecipitation Junin virus Protein Binding RNA, Viral Vero Cells Viral Proteins Virus Replication Arenavirus Junin virus RNA viruses Heterogeneous nuclear ribonucleoproteins A and B (hnRNPs A/B), cellular RNA-binding proteins that participate in splicing, trafficking, translation and turnover of mRNAs, have been implicated in the life cycles of several cytoplasmic RNA viruses. Here, we demonstrate that silencing of hnRNPs A1 and A2 significantly reduces the replication of the arenavirus Juni{dotless} ́n virus (JUNV), the aetiological agent of Argentine haemorrhagic fever. While acute JUNV infection did not modify total levels of expression of hnRNPs A/B in comparison with uninfected cells, non-cytopathic persistent infection exhibited low levels of these cell proteins. Furthermore, acutely infected cells showed a cytoplasmic relocalization of overexpressed hnRNP A1, probably related to the involvement of this protein in virus replicative cycle. This cytoplasmic accumulation was also observed in cells expressing viral nucleoprotein (N), and co-immunoprecipitation studies revealed the interaction between hnRNP A1 and N protein. By contrast, a predominantly nuclear distribution of overexpressed hnRNP A1 was found during persistent infection, even in the presence of endogenous or overexpressed N protein, indicating a differential modulation of nucleo-cytoplasmic trafficking in acute and persistent JUNV infections. © 2011 SGM. Fil:Maeto, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Knott, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Linero, F.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ellenberg, P.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Scolaro, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Castilla, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221317_v92_n9_p2181_Maeto http://hdl.handle.net/20.500.12110/paper_00221317_v92_n9_p2181_Maeto |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
cell protein ribonucleoprotein ribonucleoprotein A1 ribonucleoprotein A2 ribonucleoprotein B small interfering RNA unclassified drug virus nucleoprotein Argentine hemorrhagic fever article cellular distribution controlled study gene overexpression immunoprecipitation Junin virus nonhuman nucleocytoplasmic transport persistent infection priority journal protein expression protein protein interaction Vero cell virus cell interaction virus replication Active Transport, Cell Nucleus Animals Cercopithecus aethiops Heterogeneous-Nuclear Ribonucleoprotein Group A-B Host-Pathogen Interactions Immunoprecipitation Junin virus Protein Binding RNA, Viral Vero Cells Viral Proteins Virus Replication Arenavirus Junin virus RNA viruses |
spellingShingle |
cell protein ribonucleoprotein ribonucleoprotein A1 ribonucleoprotein A2 ribonucleoprotein B small interfering RNA unclassified drug virus nucleoprotein Argentine hemorrhagic fever article cellular distribution controlled study gene overexpression immunoprecipitation Junin virus nonhuman nucleocytoplasmic transport persistent infection priority journal protein expression protein protein interaction Vero cell virus cell interaction virus replication Active Transport, Cell Nucleus Animals Cercopithecus aethiops Heterogeneous-Nuclear Ribonucleoprotein Group A-B Host-Pathogen Interactions Immunoprecipitation Junin virus Protein Binding RNA, Viral Vero Cells Viral Proteins Virus Replication Arenavirus Junin virus RNA viruses Maeto, Cynthia A. Knott, María Elena Linero, Florencia N. Ellenberg, Paula C. Scolaro, Luis Alberto Castilla, Viviana Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B |
topic_facet |
cell protein ribonucleoprotein ribonucleoprotein A1 ribonucleoprotein A2 ribonucleoprotein B small interfering RNA unclassified drug virus nucleoprotein Argentine hemorrhagic fever article cellular distribution controlled study gene overexpression immunoprecipitation Junin virus nonhuman nucleocytoplasmic transport persistent infection priority journal protein expression protein protein interaction Vero cell virus cell interaction virus replication Active Transport, Cell Nucleus Animals Cercopithecus aethiops Heterogeneous-Nuclear Ribonucleoprotein Group A-B Host-Pathogen Interactions Immunoprecipitation Junin virus Protein Binding RNA, Viral Vero Cells Viral Proteins Virus Replication Arenavirus Junin virus RNA viruses |
description |
Heterogeneous nuclear ribonucleoproteins A and B (hnRNPs A/B), cellular RNA-binding proteins that participate in splicing, trafficking, translation and turnover of mRNAs, have been implicated in the life cycles of several cytoplasmic RNA viruses. Here, we demonstrate that silencing of hnRNPs A1 and A2 significantly reduces the replication of the arenavirus Juni{dotless} ́n virus (JUNV), the aetiological agent of Argentine haemorrhagic fever. While acute JUNV infection did not modify total levels of expression of hnRNPs A/B in comparison with uninfected cells, non-cytopathic persistent infection exhibited low levels of these cell proteins. Furthermore, acutely infected cells showed a cytoplasmic relocalization of overexpressed hnRNP A1, probably related to the involvement of this protein in virus replicative cycle. This cytoplasmic accumulation was also observed in cells expressing viral nucleoprotein (N), and co-immunoprecipitation studies revealed the interaction between hnRNP A1 and N protein. By contrast, a predominantly nuclear distribution of overexpressed hnRNP A1 was found during persistent infection, even in the presence of endogenous or overexpressed N protein, indicating a differential modulation of nucleo-cytoplasmic trafficking in acute and persistent JUNV infections. © 2011 SGM. |
author |
Maeto, Cynthia A. Knott, María Elena Linero, Florencia N. Ellenberg, Paula C. Scolaro, Luis Alberto Castilla, Viviana |
author_facet |
Maeto, Cynthia A. Knott, María Elena Linero, Florencia N. Ellenberg, Paula C. Scolaro, Luis Alberto Castilla, Viviana |
author_sort |
Maeto, Cynthia A. |
title |
Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B |
title_short |
Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B |
title_full |
Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B |
title_fullStr |
Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B |
title_full_unstemmed |
Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B |
title_sort |
differential effect of acute and persistent junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type a and b |
publishDate |
2011 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221317_v92_n9_p2181_Maeto http://hdl.handle.net/20.500.12110/paper_00221317_v92_n9_p2181_Maeto |
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