Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B

Heterogeneous nuclear ribonucleoproteins A and B (hnRNPs A/B), cellular RNA-binding proteins that participate in splicing, trafficking, translation and turnover of mRNAs, have been implicated in the life cycles of several cytoplasmic RNA viruses. Here, we demonstrate that silencing of hnRNPs A1 and...

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Autores principales: Maeto, Cynthia A., Knott, María Elena, Linero, Florencia N., Ellenberg, Paula C., Scolaro, Luis Alberto, Castilla, Viviana
Publicado: 2011
Materias:
cell protein
ribonucleoprotein
ribonucleoprotein A1
ribonucleoprotein A2
ribonucleoprotein B
small interfering RNA
unclassified drug
virus nucleoprotein
Argentine hemorrhagic fever
article
cellular distribution
controlled study
gene overexpression
immunoprecipitation
Junin virus
nonhuman
nucleocytoplasmic transport
persistent infection
priority journal
protein expression
protein protein interaction
Vero cell
virus cell interaction
virus replication
Active Transport, Cell Nucleus
Animals
Cercopithecus aethiops
Heterogeneous-Nuclear Ribonucleoprotein Group A-B
Host-Pathogen Interactions
Immunoprecipitation
Protein Binding
RNA, Viral
Vero Cells
Viral Proteins
Virus Replication
Arenavirus
RNA viruses
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221317_v92_n9_p2181_Maeto
http://hdl.handle.net/20.500.12110/paper_00221317_v92_n9_p2181_Maeto
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00221317_v92_n9_p2181_Maeto
record_format dspace
spelling paper:paper_00221317_v92_n9_p2181_Maeto2023-06-08T14:46:34Z Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B Maeto, Cynthia A. Knott, María Elena Linero, Florencia N. Ellenberg, Paula C. Scolaro, Luis Alberto Castilla, Viviana cell protein ribonucleoprotein ribonucleoprotein A1 ribonucleoprotein A2 ribonucleoprotein B small interfering RNA unclassified drug virus nucleoprotein Argentine hemorrhagic fever article cellular distribution controlled study gene overexpression immunoprecipitation Junin virus nonhuman nucleocytoplasmic transport persistent infection priority journal protein expression protein protein interaction Vero cell virus cell interaction virus replication Active Transport, Cell Nucleus Animals Cercopithecus aethiops Heterogeneous-Nuclear Ribonucleoprotein Group A-B Host-Pathogen Interactions Immunoprecipitation Junin virus Protein Binding RNA, Viral Vero Cells Viral Proteins Virus Replication Arenavirus Junin virus RNA viruses Heterogeneous nuclear ribonucleoproteins A and B (hnRNPs A/B), cellular RNA-binding proteins that participate in splicing, trafficking, translation and turnover of mRNAs, have been implicated in the life cycles of several cytoplasmic RNA viruses. Here, we demonstrate that silencing of hnRNPs A1 and A2 significantly reduces the replication of the arenavirus Juni{dotless} ́n virus (JUNV), the aetiological agent of Argentine haemorrhagic fever. While acute JUNV infection did not modify total levels of expression of hnRNPs A/B in comparison with uninfected cells, non-cytopathic persistent infection exhibited low levels of these cell proteins. Furthermore, acutely infected cells showed a cytoplasmic relocalization of overexpressed hnRNP A1, probably related to the involvement of this protein in virus replicative cycle. This cytoplasmic accumulation was also observed in cells expressing viral nucleoprotein (N), and co-immunoprecipitation studies revealed the interaction between hnRNP A1 and N protein. By contrast, a predominantly nuclear distribution of overexpressed hnRNP A1 was found during persistent infection, even in the presence of endogenous or overexpressed N protein, indicating a differential modulation of nucleo-cytoplasmic trafficking in acute and persistent JUNV infections. © 2011 SGM. Fil:Maeto, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Knott, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Linero, F.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ellenberg, P.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Scolaro, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Castilla, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221317_v92_n9_p2181_Maeto http://hdl.handle.net/20.500.12110/paper_00221317_v92_n9_p2181_Maeto
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic cell protein
ribonucleoprotein
ribonucleoprotein A1
ribonucleoprotein A2
ribonucleoprotein B
small interfering RNA
unclassified drug
virus nucleoprotein
Argentine hemorrhagic fever
article
cellular distribution
controlled study
gene overexpression
immunoprecipitation
Junin virus
nonhuman
nucleocytoplasmic transport
persistent infection
priority journal
protein expression
protein protein interaction
Vero cell
virus cell interaction
virus replication
Active Transport, Cell Nucleus
Animals
Cercopithecus aethiops
Heterogeneous-Nuclear Ribonucleoprotein Group A-B
Host-Pathogen Interactions
Immunoprecipitation
Junin virus
Protein Binding
RNA, Viral
Vero Cells
Viral Proteins
Virus Replication
Arenavirus
Junin virus
RNA viruses
spellingShingle cell protein
ribonucleoprotein
ribonucleoprotein A1
ribonucleoprotein A2
ribonucleoprotein B
small interfering RNA
unclassified drug
virus nucleoprotein
Argentine hemorrhagic fever
article
cellular distribution
controlled study
gene overexpression
immunoprecipitation
Junin virus
nonhuman
nucleocytoplasmic transport
persistent infection
priority journal
protein expression
protein protein interaction
Vero cell
virus cell interaction
virus replication
Active Transport, Cell Nucleus
Animals
Cercopithecus aethiops
Heterogeneous-Nuclear Ribonucleoprotein Group A-B
Host-Pathogen Interactions
Immunoprecipitation
Junin virus
Protein Binding
RNA, Viral
Vero Cells
Viral Proteins
Virus Replication
Arenavirus
Junin virus
RNA viruses
Maeto, Cynthia A.
Knott, María Elena
Linero, Florencia N.
Ellenberg, Paula C.
Scolaro, Luis Alberto
Castilla, Viviana
Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B
topic_facet cell protein
ribonucleoprotein
ribonucleoprotein A1
ribonucleoprotein A2
ribonucleoprotein B
small interfering RNA
unclassified drug
virus nucleoprotein
Argentine hemorrhagic fever
article
cellular distribution
controlled study
gene overexpression
immunoprecipitation
Junin virus
nonhuman
nucleocytoplasmic transport
persistent infection
priority journal
protein expression
protein protein interaction
Vero cell
virus cell interaction
virus replication
Active Transport, Cell Nucleus
Animals
Cercopithecus aethiops
Heterogeneous-Nuclear Ribonucleoprotein Group A-B
Host-Pathogen Interactions
Immunoprecipitation
Junin virus
Protein Binding
RNA, Viral
Vero Cells
Viral Proteins
Virus Replication
Arenavirus
Junin virus
RNA viruses
description Heterogeneous nuclear ribonucleoproteins A and B (hnRNPs A/B), cellular RNA-binding proteins that participate in splicing, trafficking, translation and turnover of mRNAs, have been implicated in the life cycles of several cytoplasmic RNA viruses. Here, we demonstrate that silencing of hnRNPs A1 and A2 significantly reduces the replication of the arenavirus Juni{dotless} ́n virus (JUNV), the aetiological agent of Argentine haemorrhagic fever. While acute JUNV infection did not modify total levels of expression of hnRNPs A/B in comparison with uninfected cells, non-cytopathic persistent infection exhibited low levels of these cell proteins. Furthermore, acutely infected cells showed a cytoplasmic relocalization of overexpressed hnRNP A1, probably related to the involvement of this protein in virus replicative cycle. This cytoplasmic accumulation was also observed in cells expressing viral nucleoprotein (N), and co-immunoprecipitation studies revealed the interaction between hnRNP A1 and N protein. By contrast, a predominantly nuclear distribution of overexpressed hnRNP A1 was found during persistent infection, even in the presence of endogenous or overexpressed N protein, indicating a differential modulation of nucleo-cytoplasmic trafficking in acute and persistent JUNV infections. © 2011 SGM.
author Maeto, Cynthia A.
Knott, María Elena
Linero, Florencia N.
Ellenberg, Paula C.
Scolaro, Luis Alberto
Castilla, Viviana
author_facet Maeto, Cynthia A.
Knott, María Elena
Linero, Florencia N.
Ellenberg, Paula C.
Scolaro, Luis Alberto
Castilla, Viviana
author_sort Maeto, Cynthia A.
title Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B
title_short Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B
title_full Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B
title_fullStr Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B
title_full_unstemmed Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B
title_sort differential effect of acute and persistent junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type a and b
publishDate 2011
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221317_v92_n9_p2181_Maeto
http://hdl.handle.net/20.500.12110/paper_00221317_v92_n9_p2181_Maeto
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