Nitric oxide inhibits leydig cell steroidogenesis
Testicular macrophages as well as endothelial cells, which are intimately associated with Leydig cells, constitute a potential source of paracrine nitric oxide (NO) in the testis. In the present study, we investigated the effect of NO donors on MA-10 murine Leydig tumor cell line and rat Leydig cell...
Guardado en:
Autores principales: | , , |
---|---|
Publicado: |
1996
|
Materias: | |
Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v137_n12_p5337_DelPunta http://hdl.handle.net/20.500.12110/paper_00137227_v137_n12_p5337_DelPunta |
Aporte de: |
id |
paper:paper_00137227_v137_n12_p5337_DelPunta |
---|---|
record_format |
dspace |
spelling |
paper:paper_00137227_v137_n12_p5337_DelPunta2023-06-08T14:36:06Z Nitric oxide inhibits leydig cell steroidogenesis Del Punta, Karina Charreau, Eduardo Hernán Pignataro, Omar Pedro cholesterol cholesterol monooxygenase (side chain cleaving) chorionic gonadotropin cyclic AMP cyclic GMP cytochrome P450 diethylamine diethylenetriamine hemoprotein nitric oxide nitric oxide donor pregnenolone steroid animal cell article autocrine effect controlled study Leydig cell Leydig cell tumor male mouse nonhuman priority journal rat second messenger steroidogenesis testosterone synthesis Testicular macrophages as well as endothelial cells, which are intimately associated with Leydig cells, constitute a potential source of paracrine nitric oxide (NO) in the testis. In the present study, we investigated the effect of NO donors on MA-10 murine Leydig tumor cell line and rat Leydig cell steroidogenesis. We show that NO donors inhibit human CG- induced steroidogenesis in both type of cells. We also studied NO mechanism of action. Contrary to what is observed in many other systems, NO inhibitory effect on Leydig cell steroidogenesis is not mediated by cyclic GMP (cGMP) because NO fails to increase cGMP production, and cGMP analogs do not reproduce NO effect. NO does not modify the production of cAMP, the main second messenger that mediates gonadotropin action. When we studied NO effect over the steroidogenic pathway in MA-10 cells, we found that NO was inhibiting the conversion of cholesterol to pregnenolone. Taken together these results show an inhibitory effect of NO donors on Leydig cell steroidogenesis, and suggest that NO can be directly inhibiting cholesterol side-chain cleavage enzyme (cytochrome P450(scc)) as it does with other heme proteins, including different cytochromes P459. Fil:Del Punta, K. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Charreau, E.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pignataro, O.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1996 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v137_n12_p5337_DelPunta http://hdl.handle.net/20.500.12110/paper_00137227_v137_n12_p5337_DelPunta |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
cholesterol cholesterol monooxygenase (side chain cleaving) chorionic gonadotropin cyclic AMP cyclic GMP cytochrome P450 diethylamine diethylenetriamine hemoprotein nitric oxide nitric oxide donor pregnenolone steroid animal cell article autocrine effect controlled study Leydig cell Leydig cell tumor male mouse nonhuman priority journal rat second messenger steroidogenesis testosterone synthesis |
spellingShingle |
cholesterol cholesterol monooxygenase (side chain cleaving) chorionic gonadotropin cyclic AMP cyclic GMP cytochrome P450 diethylamine diethylenetriamine hemoprotein nitric oxide nitric oxide donor pregnenolone steroid animal cell article autocrine effect controlled study Leydig cell Leydig cell tumor male mouse nonhuman priority journal rat second messenger steroidogenesis testosterone synthesis Del Punta, Karina Charreau, Eduardo Hernán Pignataro, Omar Pedro Nitric oxide inhibits leydig cell steroidogenesis |
topic_facet |
cholesterol cholesterol monooxygenase (side chain cleaving) chorionic gonadotropin cyclic AMP cyclic GMP cytochrome P450 diethylamine diethylenetriamine hemoprotein nitric oxide nitric oxide donor pregnenolone steroid animal cell article autocrine effect controlled study Leydig cell Leydig cell tumor male mouse nonhuman priority journal rat second messenger steroidogenesis testosterone synthesis |
description |
Testicular macrophages as well as endothelial cells, which are intimately associated with Leydig cells, constitute a potential source of paracrine nitric oxide (NO) in the testis. In the present study, we investigated the effect of NO donors on MA-10 murine Leydig tumor cell line and rat Leydig cell steroidogenesis. We show that NO donors inhibit human CG- induced steroidogenesis in both type of cells. We also studied NO mechanism of action. Contrary to what is observed in many other systems, NO inhibitory effect on Leydig cell steroidogenesis is not mediated by cyclic GMP (cGMP) because NO fails to increase cGMP production, and cGMP analogs do not reproduce NO effect. NO does not modify the production of cAMP, the main second messenger that mediates gonadotropin action. When we studied NO effect over the steroidogenic pathway in MA-10 cells, we found that NO was inhibiting the conversion of cholesterol to pregnenolone. Taken together these results show an inhibitory effect of NO donors on Leydig cell steroidogenesis, and suggest that NO can be directly inhibiting cholesterol side-chain cleavage enzyme (cytochrome P450(scc)) as it does with other heme proteins, including different cytochromes P459. |
author |
Del Punta, Karina Charreau, Eduardo Hernán Pignataro, Omar Pedro |
author_facet |
Del Punta, Karina Charreau, Eduardo Hernán Pignataro, Omar Pedro |
author_sort |
Del Punta, Karina |
title |
Nitric oxide inhibits leydig cell steroidogenesis |
title_short |
Nitric oxide inhibits leydig cell steroidogenesis |
title_full |
Nitric oxide inhibits leydig cell steroidogenesis |
title_fullStr |
Nitric oxide inhibits leydig cell steroidogenesis |
title_full_unstemmed |
Nitric oxide inhibits leydig cell steroidogenesis |
title_sort |
nitric oxide inhibits leydig cell steroidogenesis |
publishDate |
1996 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v137_n12_p5337_DelPunta http://hdl.handle.net/20.500.12110/paper_00137227_v137_n12_p5337_DelPunta |
work_keys_str_mv |
AT delpuntakarina nitricoxideinhibitsleydigcellsteroidogenesis AT charreaueduardohernan nitricoxideinhibitsleydigcellsteroidogenesis AT pignataroomarpedro nitricoxideinhibitsleydigcellsteroidogenesis |
_version_ |
1768542919591985152 |