Nitric oxide inhibits leydig cell steroidogenesis

Testicular macrophages as well as endothelial cells, which are intimately associated with Leydig cells, constitute a potential source of paracrine nitric oxide (NO) in the testis. In the present study, we investigated the effect of NO donors on MA-10 murine Leydig tumor cell line and rat Leydig cell...

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Autores principales: Del Punta, Karina, Charreau, Eduardo Hernán, Pignataro, Omar Pedro
Publicado: 1996
Materias:
cholesterol
cholesterol monooxygenase (side chain cleaving)
chorionic gonadotropin
cyclic AMP
cyclic GMP
cytochrome P450
diethylamine
diethylenetriamine
hemoprotein
nitric oxide
nitric oxide donor
pregnenolone
steroid
animal cell
article
autocrine effect
controlled study
Leydig cell
Leydig cell tumor
male
mouse
nonhuman
priority journal
rat
second messenger
steroidogenesis
testosterone synthesis
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v137_n12_p5337_DelPunta
http://hdl.handle.net/20.500.12110/paper_00137227_v137_n12_p5337_DelPunta
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00137227_v137_n12_p5337_DelPunta
record_format dspace
spelling paper:paper_00137227_v137_n12_p5337_DelPunta2023-06-08T14:36:06Z Nitric oxide inhibits leydig cell steroidogenesis Del Punta, Karina Charreau, Eduardo Hernán Pignataro, Omar Pedro cholesterol cholesterol monooxygenase (side chain cleaving) chorionic gonadotropin cyclic AMP cyclic GMP cytochrome P450 diethylamine diethylenetriamine hemoprotein nitric oxide nitric oxide donor pregnenolone steroid animal cell article autocrine effect controlled study Leydig cell Leydig cell tumor male mouse nonhuman priority journal rat second messenger steroidogenesis testosterone synthesis Testicular macrophages as well as endothelial cells, which are intimately associated with Leydig cells, constitute a potential source of paracrine nitric oxide (NO) in the testis. In the present study, we investigated the effect of NO donors on MA-10 murine Leydig tumor cell line and rat Leydig cell steroidogenesis. We show that NO donors inhibit human CG- induced steroidogenesis in both type of cells. We also studied NO mechanism of action. Contrary to what is observed in many other systems, NO inhibitory effect on Leydig cell steroidogenesis is not mediated by cyclic GMP (cGMP) because NO fails to increase cGMP production, and cGMP analogs do not reproduce NO effect. NO does not modify the production of cAMP, the main second messenger that mediates gonadotropin action. When we studied NO effect over the steroidogenic pathway in MA-10 cells, we found that NO was inhibiting the conversion of cholesterol to pregnenolone. Taken together these results show an inhibitory effect of NO donors on Leydig cell steroidogenesis, and suggest that NO can be directly inhibiting cholesterol side-chain cleavage enzyme (cytochrome P450(scc)) as it does with other heme proteins, including different cytochromes P459. Fil:Del Punta, K. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Charreau, E.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pignataro, O.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1996 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v137_n12_p5337_DelPunta http://hdl.handle.net/20.500.12110/paper_00137227_v137_n12_p5337_DelPunta
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic cholesterol
cholesterol monooxygenase (side chain cleaving)
chorionic gonadotropin
cyclic AMP
cyclic GMP
cytochrome P450
diethylamine
diethylenetriamine
hemoprotein
nitric oxide
nitric oxide donor
pregnenolone
steroid
animal cell
article
autocrine effect
controlled study
Leydig cell
Leydig cell tumor
male
mouse
nonhuman
priority journal
rat
second messenger
steroidogenesis
testosterone synthesis
spellingShingle cholesterol
cholesterol monooxygenase (side chain cleaving)
chorionic gonadotropin
cyclic AMP
cyclic GMP
cytochrome P450
diethylamine
diethylenetriamine
hemoprotein
nitric oxide
nitric oxide donor
pregnenolone
steroid
animal cell
article
autocrine effect
controlled study
Leydig cell
Leydig cell tumor
male
mouse
nonhuman
priority journal
rat
second messenger
steroidogenesis
testosterone synthesis
Del Punta, Karina
Charreau, Eduardo Hernán
Pignataro, Omar Pedro
Nitric oxide inhibits leydig cell steroidogenesis
topic_facet cholesterol
cholesterol monooxygenase (side chain cleaving)
chorionic gonadotropin
cyclic AMP
cyclic GMP
cytochrome P450
diethylamine
diethylenetriamine
hemoprotein
nitric oxide
nitric oxide donor
pregnenolone
steroid
animal cell
article
autocrine effect
controlled study
Leydig cell
Leydig cell tumor
male
mouse
nonhuman
priority journal
rat
second messenger
steroidogenesis
testosterone synthesis
description Testicular macrophages as well as endothelial cells, which are intimately associated with Leydig cells, constitute a potential source of paracrine nitric oxide (NO) in the testis. In the present study, we investigated the effect of NO donors on MA-10 murine Leydig tumor cell line and rat Leydig cell steroidogenesis. We show that NO donors inhibit human CG- induced steroidogenesis in both type of cells. We also studied NO mechanism of action. Contrary to what is observed in many other systems, NO inhibitory effect on Leydig cell steroidogenesis is not mediated by cyclic GMP (cGMP) because NO fails to increase cGMP production, and cGMP analogs do not reproduce NO effect. NO does not modify the production of cAMP, the main second messenger that mediates gonadotropin action. When we studied NO effect over the steroidogenic pathway in MA-10 cells, we found that NO was inhibiting the conversion of cholesterol to pregnenolone. Taken together these results show an inhibitory effect of NO donors on Leydig cell steroidogenesis, and suggest that NO can be directly inhibiting cholesterol side-chain cleavage enzyme (cytochrome P450(scc)) as it does with other heme proteins, including different cytochromes P459.
author Del Punta, Karina
Charreau, Eduardo Hernán
Pignataro, Omar Pedro
author_facet Del Punta, Karina
Charreau, Eduardo Hernán
Pignataro, Omar Pedro
author_sort Del Punta, Karina
title Nitric oxide inhibits leydig cell steroidogenesis
title_short Nitric oxide inhibits leydig cell steroidogenesis
title_full Nitric oxide inhibits leydig cell steroidogenesis
title_fullStr Nitric oxide inhibits leydig cell steroidogenesis
title_full_unstemmed Nitric oxide inhibits leydig cell steroidogenesis
title_sort nitric oxide inhibits leydig cell steroidogenesis
publishDate 1996
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v137_n12_p5337_DelPunta
http://hdl.handle.net/20.500.12110/paper_00137227_v137_n12_p5337_DelPunta
work_keys_str_mv AT delpuntakarina nitricoxideinhibitsleydigcellsteroidogenesis
AT charreaueduardohernan nitricoxideinhibitsleydigcellsteroidogenesis
AT pignataroomarpedro nitricoxideinhibitsleydigcellsteroidogenesis
_version_ 1768542919591985152

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