Phytochemistry of Tagetes minuta L. (Asteraceae) from Córdoba, Argentina: comparative study between essential oil and HS-SPME analyses

A headspace solid-phase microextraction (HS-SPME) method followed by gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detection (GC-FID) is described for the analysis of volatile compounds in Tagetes minuta L. Five types of SPME commercial fibers including PA, PDM...

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Autores principales: Vázquez, Ana María, Demmel, Gabriela Inés, Criado, Silvia Gertrudis, Aimar, Mario Leandro, Cantero, Juan, Rossi, Laura I., Velasco, Manuel I.
Formato: Artículo acceptedVersion
Lenguaje:Español
Publicado: Universidad de Santiago de Chile 2011
Materias:
QD Química
Acceso en línea:http://pa.bibdigital.ucc.edu.ar/3457/1/A_V%C3%A1zquez_Demmel_Criado_Aimar_Cantero_Rossi_Velasco.pdf
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Producción Académica Universidad Católica de Córdoba (UCCor) de Universidad Católica de Córdoba
id I38-R144-3457
record_format dspace
institution Universidad Católica de Córdoba
institution_str I-38
repository_str R-144
collection Producción Académica Universidad Católica de Córdoba (UCCor)
language Español
orig_language_str_mv spa
topic QD Química
spellingShingle QD Química
Vázquez, Ana María
Demmel, Gabriela Inés
Criado, Silvia Gertrudis
Aimar, Mario Leandro
Cantero, Juan
Rossi, Laura I.
Velasco, Manuel I.
Phytochemistry of Tagetes minuta L. (Asteraceae) from Córdoba, Argentina: comparative study between essential oil and HS-SPME analyses
topic_facet QD Química
description A headspace solid-phase microextraction (HS-SPME) method followed by gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detection (GC-FID) is described for the analysis of volatile compounds in Tagetes minuta L. Five types of SPME commercial fibers including PA, PDMS, CAR-PDMS, PDMS-DVB and DVB-CAR-PDMS were investigated and the best extraction was achieved with the mixed fiber DVB-CAR-PDMS. Parameters for HS-SPME in terms of equilibrium time of HS, fiber exposition time and extraction temperature were also investigated. Additionally, the composition of inflorescences, leaves and stems was also studied separately by HS-SPME. As a result, 68 compounds were determined and 53 were identified. A comparison was made between results obtained by HS-SPME-GC-MS and steam distillation of essential oil of the aerial parts of the plant. In both analyses, the major components were: cis-tagetenone and trans-tagetenone. Using much smaller samples, a shorter extraction time and a simpler procedure, the HS-SPME method can achieve similar results to those obtained by EO analysis. In conclusion, the HS-SPME method is simple, rapid, effective and free of solvent, and can be used for the analysis of volatile compounds in samples of different populations of T. minuta.
format Artículo
Artículo
acceptedVersion
author Vázquez, Ana María
Demmel, Gabriela Inés
Criado, Silvia Gertrudis
Aimar, Mario Leandro
Cantero, Juan
Rossi, Laura I.
Velasco, Manuel I.
author_facet Vázquez, Ana María
Demmel, Gabriela Inés
Criado, Silvia Gertrudis
Aimar, Mario Leandro
Cantero, Juan
Rossi, Laura I.
Velasco, Manuel I.
author_sort Vázquez, Ana María
title Phytochemistry of Tagetes minuta L. (Asteraceae) from Córdoba, Argentina: comparative study between essential oil and HS-SPME analyses
title_short Phytochemistry of Tagetes minuta L. (Asteraceae) from Córdoba, Argentina: comparative study between essential oil and HS-SPME analyses
title_full Phytochemistry of Tagetes minuta L. (Asteraceae) from Córdoba, Argentina: comparative study between essential oil and HS-SPME analyses
title_fullStr Phytochemistry of Tagetes minuta L. (Asteraceae) from Córdoba, Argentina: comparative study between essential oil and HS-SPME analyses
title_full_unstemmed Phytochemistry of Tagetes minuta L. (Asteraceae) from Córdoba, Argentina: comparative study between essential oil and HS-SPME analyses
title_sort phytochemistry of tagetes minuta l. (asteraceae) from córdoba, argentina: comparative study between essential oil and hs-spme analyses
publisher Universidad de Santiago de Chile
publishDate 2011
url http://pa.bibdigital.ucc.edu.ar/3457/1/A_V%C3%A1zquez_Demmel_Criado_Aimar_Cantero_Rossi_Velasco.pdf
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