A 5′ RNA element promotes dengue virus RNA synthesis on a circular genome
The mechanisms of RNA replication of plus-strand RNA viruses are still unclear. Here, we identified the first promoter element for RNA synthesis described in a flavivirus. Using dengue virus as a model, we found that the viral RdRp discriminates the viral RNA by specific recognition of a 5′ element...
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Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_08909369_v20_n16_p2238_Filomatori http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_08909369_v20_n16_p2238_Filomatori_oai |
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I28-R145-paper_08909369_v20_n16_p2238_Filomatori_oai2020-10-19 Filomatori, C.V. Lodeiro, M.F. Alvarez, D.E. Samsa, M.M. Pietrasanta, L. Gamarnik, A.V. 2006 The mechanisms of RNA replication of plus-strand RNA viruses are still unclear. Here, we identified the first promoter element for RNA synthesis described in a flavivirus. Using dengue virus as a model, we found that the viral RdRp discriminates the viral RNA by specific recognition of a 5′ element named SLA. We demonstrated that RNA-RNA interactions between 5′ and 3′ end sequences of the viral genome enhance dengue virus RNA synthesis only in the presence of an intact SLA. We propose a novel mechanism for minus-strand RNA synthesis in which the viral polymerase binds SLA at the 5′ end of the genome and reaches the site of initiation at the 3′ end via long-range RNA-RNA interactions. These findings provide an explanation for the strict requirement of dengue virus genome cyclization during viral replication. © 2006 by Cold Spring Harbor Laboratory Press. application/pdf http://hdl.handle.net/20.500.12110/paper_08909369_v20_n16_p2238_Filomatori info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar Genes Dev. 2006;20(16):2238-2249 AFM Flavivirus RNA cyclization RNA-dependent RNA polymerase Viral RNA synthesis article controlled study Dengue virus genome nonhuman priority journal RNA replication RNA synthesis RNA virus atomic force microscopy biosynthesis chemistry conformation Dengue virus genetics metabolism physiology regulatory RNA sequence virus genome virus replication Dengue virus Flavivirus RNA viruses NS5 protein, flavivirus RNA directed RNA polymerase virus protein virus RNA Dengue Virus Genome, Viral Microscopy, Atomic Force Nucleic Acid Conformation Regulatory Sequences, Ribonucleic Acid RNA Replicase RNA, Viral Viral Nonstructural Proteins Virus Replication A 5′ RNA element promotes dengue virus RNA synthesis on a circular genome info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_08909369_v20_n16_p2238_Filomatori_oai |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-145 |
collection |
Repositorio Digital de la Universidad de Buenos Aires (UBA) |
topic |
AFM Flavivirus RNA cyclization RNA-dependent RNA polymerase Viral RNA synthesis article controlled study Dengue virus genome nonhuman priority journal RNA replication RNA synthesis RNA virus atomic force microscopy biosynthesis chemistry conformation Dengue virus genetics metabolism physiology regulatory RNA sequence virus genome virus replication Dengue virus Flavivirus RNA viruses NS5 protein, flavivirus RNA directed RNA polymerase virus protein virus RNA Dengue Virus Genome, Viral Microscopy, Atomic Force Nucleic Acid Conformation Regulatory Sequences, Ribonucleic Acid RNA Replicase RNA, Viral Viral Nonstructural Proteins Virus Replication |
spellingShingle |
AFM Flavivirus RNA cyclization RNA-dependent RNA polymerase Viral RNA synthesis article controlled study Dengue virus genome nonhuman priority journal RNA replication RNA synthesis RNA virus atomic force microscopy biosynthesis chemistry conformation Dengue virus genetics metabolism physiology regulatory RNA sequence virus genome virus replication Dengue virus Flavivirus RNA viruses NS5 protein, flavivirus RNA directed RNA polymerase virus protein virus RNA Dengue Virus Genome, Viral Microscopy, Atomic Force Nucleic Acid Conformation Regulatory Sequences, Ribonucleic Acid RNA Replicase RNA, Viral Viral Nonstructural Proteins Virus Replication Filomatori, C.V. Lodeiro, M.F. Alvarez, D.E. Samsa, M.M. Pietrasanta, L. Gamarnik, A.V. A 5′ RNA element promotes dengue virus RNA synthesis on a circular genome |
topic_facet |
AFM Flavivirus RNA cyclization RNA-dependent RNA polymerase Viral RNA synthesis article controlled study Dengue virus genome nonhuman priority journal RNA replication RNA synthesis RNA virus atomic force microscopy biosynthesis chemistry conformation Dengue virus genetics metabolism physiology regulatory RNA sequence virus genome virus replication Dengue virus Flavivirus RNA viruses NS5 protein, flavivirus RNA directed RNA polymerase virus protein virus RNA Dengue Virus Genome, Viral Microscopy, Atomic Force Nucleic Acid Conformation Regulatory Sequences, Ribonucleic Acid RNA Replicase RNA, Viral Viral Nonstructural Proteins Virus Replication |
description |
The mechanisms of RNA replication of plus-strand RNA viruses are still unclear. Here, we identified the first promoter element for RNA synthesis described in a flavivirus. Using dengue virus as a model, we found that the viral RdRp discriminates the viral RNA by specific recognition of a 5′ element named SLA. We demonstrated that RNA-RNA interactions between 5′ and 3′ end sequences of the viral genome enhance dengue virus RNA synthesis only in the presence of an intact SLA. We propose a novel mechanism for minus-strand RNA synthesis in which the viral polymerase binds SLA at the 5′ end of the genome and reaches the site of initiation at the 3′ end via long-range RNA-RNA interactions. These findings provide an explanation for the strict requirement of dengue virus genome cyclization during viral replication. © 2006 by Cold Spring Harbor Laboratory Press. |
format |
Artículo Artículo publishedVersion |
author |
Filomatori, C.V. Lodeiro, M.F. Alvarez, D.E. Samsa, M.M. Pietrasanta, L. Gamarnik, A.V. |
author_facet |
Filomatori, C.V. Lodeiro, M.F. Alvarez, D.E. Samsa, M.M. Pietrasanta, L. Gamarnik, A.V. |
author_sort |
Filomatori, C.V. |
title |
A 5′ RNA element promotes dengue virus RNA synthesis on a circular genome |
title_short |
A 5′ RNA element promotes dengue virus RNA synthesis on a circular genome |
title_full |
A 5′ RNA element promotes dengue virus RNA synthesis on a circular genome |
title_fullStr |
A 5′ RNA element promotes dengue virus RNA synthesis on a circular genome |
title_full_unstemmed |
A 5′ RNA element promotes dengue virus RNA synthesis on a circular genome |
title_sort |
5′ rna element promotes dengue virus rna synthesis on a circular genome |
publishDate |
2006 |
url |
http://hdl.handle.net/20.500.12110/paper_08909369_v20_n16_p2238_Filomatori http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_08909369_v20_n16_p2238_Filomatori_oai |
work_keys_str_mv |
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