Mineral Phosphate Solubilization in Burkholderia tropica Involves an Inducible PQQ-Glucose Dehydrogenase

Aims: The objective of this work was to provide knowledgement about the mechanism and regulation of the mineral phosphate solubilization in Burkholderia. tropica. To this end, the expression of the direct extracellular oxidative pathway in B. tropica was studied using different culture approaches.St...

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Detalles Bibliográficos
Autores principales: Bernabeu, Pamela Romina, García, Sabrina Soledad, Garcia Ferreyra, Gimena, Guidi, Verónica Inés, Galar, María Lina, Boiardi, José Luis, Luna, María Flavia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2016
Materias:
Química
Biología
Burkholderia tropica
Phosphate solubilization
PQQ-GDH
Nitrogen fixation
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/99818
https://ri.conicet.gov.ar/11336/40241
http://sciencedomain.org/issue/1689
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-99818
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Biología
Burkholderia tropica
Phosphate solubilization
PQQ-GDH
Nitrogen fixation
spellingShingle Química
Biología
Burkholderia tropica
Phosphate solubilization
PQQ-GDH
Nitrogen fixation
Bernabeu, Pamela Romina
García, Sabrina Soledad
Garcia Ferreyra, Gimena
Guidi, Verónica Inés
Galar, María Lina
Boiardi, José Luis
Luna, María Flavia
Mineral Phosphate Solubilization in Burkholderia tropica Involves an Inducible PQQ-Glucose Dehydrogenase
topic_facet Química
Biología
Burkholderia tropica
Phosphate solubilization
PQQ-GDH
Nitrogen fixation
description Aims: The objective of this work was to provide knowledgement about the mechanism and regulation of the mineral phosphate solubilization in Burkholderia. tropica. To this end, the expression of the direct extracellular oxidative pathway in B. tropica was studied using different culture approaches.Study Design: Plate assays and batch cultures in flasks and bioreactor were carried out in this study with B. tropica Mto-293 like target organism. The experiments were achieved at least three times with two repetitions per time.Place and Duration of Study: Departamento de Química, Centro de Investigación y Desarrollo en Fermentaciones Industriales, UNLP, CCT-La Plata-CONICET, between November 2014-2015.Methodology: Qualitative plate assays with different Carbon sources were carried out for the evaluation of Mineral Phosphate Solubilization phenotype. Batch cultures in flasks were carried out with different Carbon, Phosphorus and Nitrogen sources to determine quantitatively soluble phosphorus, gluconic acid and other ketoacids in the supernatants, and also PQQ-linked glucose and gluconate dehydrogenase activities in whole cells. Cultures with some of the conditions mentioned before were carried out in bioreactor specifically to control pH.Results: This organism was able to produce significant amounts of gluconic acid via the expression of a PQQ-GDH and also showed a significant activity of GaDH. However, the direct oxidative pathway was only observed under conditions of Phosphorus starvation and/or Nitrogen fixation.Conclusion: The Mineral Phosphate Solubilization phenotype for B. tropica can be ascribed to the expression of the direct oxidative pathway which involves the expression of an active PQQ- linked glucose dehydrogenase. Nevertheless, this pathway is not expressed constitutively in this bacterium. Environmental conditions, like low P and N availability, led to an active extracellular glucose oxidation. Therefore, mineral phosphate solubilization in B. tropica involves an inducible pyrroloquinoline quinone-linked glucose dehydrogenase. These findings may contribute to the use of this bacterium as plant growth promoting bacteria reducing the dependence on chemical fertilizer.
format Articulo
Articulo
author Bernabeu, Pamela Romina
García, Sabrina Soledad
Garcia Ferreyra, Gimena
Guidi, Verónica Inés
Galar, María Lina
Boiardi, José Luis
Luna, María Flavia
author_facet Bernabeu, Pamela Romina
García, Sabrina Soledad
Garcia Ferreyra, Gimena
Guidi, Verónica Inés
Galar, María Lina
Boiardi, José Luis
Luna, María Flavia
author_sort Bernabeu, Pamela Romina
title Mineral Phosphate Solubilization in Burkholderia tropica Involves an Inducible PQQ-Glucose Dehydrogenase
title_short Mineral Phosphate Solubilization in Burkholderia tropica Involves an Inducible PQQ-Glucose Dehydrogenase
title_full Mineral Phosphate Solubilization in Burkholderia tropica Involves an Inducible PQQ-Glucose Dehydrogenase
title_fullStr Mineral Phosphate Solubilization in Burkholderia tropica Involves an Inducible PQQ-Glucose Dehydrogenase
title_full_unstemmed Mineral Phosphate Solubilization in Burkholderia tropica Involves an Inducible PQQ-Glucose Dehydrogenase
title_sort mineral phosphate solubilization in burkholderia tropica involves an inducible pqq-glucose dehydrogenase
publishDate 2016
url http://sedici.unlp.edu.ar/handle/10915/99818
https://ri.conicet.gov.ar/11336/40241
http://sciencedomain.org/issue/1689
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