Comparación de métodos de lisis y extracción de ADN de trofozoiteos de Giardia lamblia
The efficiency of lysis procedures and DNA purification treatments of Giardia lamblia trophozoites were evaluated regarding the breaking-up efficiency, quantity and pureness of DNA, processing time and costs. Five lysis methods (distilled water and heating; distilled water, heating and proteinase K;...
Autores principales: | , , , , , |
---|---|
Formato: | Articulo |
Lenguaje: | Español |
Publicado: |
2006
|
Materias: | |
Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/99788 https://ri.conicet.gov.ar/11336/27121 |
Aporte de: |
id |
I19-R120-10915-99788 |
---|---|
record_format |
dspace |
institution |
Universidad Nacional de La Plata |
institution_str |
I-19 |
repository_str |
R-120 |
collection |
SEDICI (UNLP) |
language |
Español |
topic |
Ciencias Médicas Parásitos Giardia lamblia ADN extracción DNA extraction Lysis |
spellingShingle |
Ciencias Médicas Parásitos Giardia lamblia ADN extracción DNA extraction Lysis Molina, Nora Beatriz Polverino, Daniela Minvielle, Marta Cecilia Apezteguía, María del Carmen Aguilar, Orlando Mario Basualdo Farjat, Juan Ángel Comparación de métodos de lisis y extracción de ADN de trofozoiteos de Giardia lamblia |
topic_facet |
Ciencias Médicas Parásitos Giardia lamblia ADN extracción DNA extraction Lysis |
description |
The efficiency of lysis procedures and DNA purification treatments of Giardia lamblia trophozoites were evaluated regarding the breaking-up efficiency, quantity and pureness of DNA, processing time and costs. Five lysis methods (distilled water and heating; distilled water, heating and proteinase K; buffer D Lysis; buffer E lysis, and commercial kit), and three DNA purification methods (phenol:chloroform:isoamilic, Chelex 100 and commercial kit) were tested. The obtained data were statistically analyzed. The buffer E lysis and Chelex combination was simple and economic method which produced high DNA performance with low pureness. Commercial kit was simple and expensive method which generated low yields of DNA with appropriate purity level for molecular analysis. |
format |
Articulo Articulo |
author |
Molina, Nora Beatriz Polverino, Daniela Minvielle, Marta Cecilia Apezteguía, María del Carmen Aguilar, Orlando Mario Basualdo Farjat, Juan Ángel |
author_facet |
Molina, Nora Beatriz Polverino, Daniela Minvielle, Marta Cecilia Apezteguía, María del Carmen Aguilar, Orlando Mario Basualdo Farjat, Juan Ángel |
author_sort |
Molina, Nora Beatriz |
title |
Comparación de métodos de lisis y extracción de ADN de trofozoiteos de Giardia lamblia |
title_short |
Comparación de métodos de lisis y extracción de ADN de trofozoiteos de Giardia lamblia |
title_full |
Comparación de métodos de lisis y extracción de ADN de trofozoiteos de Giardia lamblia |
title_fullStr |
Comparación de métodos de lisis y extracción de ADN de trofozoiteos de Giardia lamblia |
title_full_unstemmed |
Comparación de métodos de lisis y extracción de ADN de trofozoiteos de Giardia lamblia |
title_sort |
comparación de métodos de lisis y extracción de adn de trofozoiteos de giardia lamblia |
publishDate |
2006 |
url |
http://sedici.unlp.edu.ar/handle/10915/99788 https://ri.conicet.gov.ar/11336/27121 |
work_keys_str_mv |
AT molinanorabeatriz comparaciondemetodosdelisisyextracciondeadndetrofozoiteosdegiardialamblia AT polverinodaniela comparaciondemetodosdelisisyextracciondeadndetrofozoiteosdegiardialamblia AT minviellemartacecilia comparaciondemetodosdelisisyextracciondeadndetrofozoiteosdegiardialamblia AT apezteguiamariadelcarmen comparaciondemetodosdelisisyextracciondeadndetrofozoiteosdegiardialamblia AT aguilarorlandomario comparaciondemetodosdelisisyextracciondeadndetrofozoiteosdegiardialamblia AT basualdofarjatjuanangel comparaciondemetodosdelisisyextracciondeadndetrofozoiteosdegiardialamblia AT molinanorabeatriz comparisonoflysismethodsanddnaextractionofgiardialambliatrophozoites AT polverinodaniela comparisonoflysismethodsanddnaextractionofgiardialambliatrophozoites AT minviellemartacecilia comparisonoflysismethodsanddnaextractionofgiardialambliatrophozoites AT apezteguiamariadelcarmen comparisonoflysismethodsanddnaextractionofgiardialambliatrophozoites AT aguilarorlandomario comparisonoflysismethodsanddnaextractionofgiardialambliatrophozoites AT basualdofarjatjuanangel comparisonoflysismethodsanddnaextractionofgiardialambliatrophozoites |
bdutipo_str |
Repositorios |
_version_ |
1764820493285720065 |