ESIPT and FRET probes for monitoring nanoparticle polymer coating stability

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Coating strategies of inorganic nanoparticles (NPs) can provide properties unavailable to the NP core alone, such as targeting, specific sensing, and increased biocompatibility. Non-covalent amphiphilic NP capping polymers function via hydrophobic interactions with surface ligands and are extensivel...

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Autores principales: Azcárate, Julio César, Díaz, Sebastián A., Fauerbach, Jonathan Arturo, Gillanders, Florencia, Rubert, Aldo Alberto, Jares, Elizabeth Andrea, Jovin, Thomas M., Fonticelli, Mariano Hernán
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2017
Materias:
Química
Tem
Nanoparticles
Coalescence
Thiols
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/99444
https://ri.conicet.gov.ar/11336/63229
https://pubs.rsc.org/en/Content/ArticleLanding/2017/NR/C7NR01787A
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-99444
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Tem
Nanoparticles
Coalescence
Thiols
spellingShingle Química
Tem
Nanoparticles
Coalescence
Thiols
Azcárate, Julio César
Díaz, Sebastián A.
Fauerbach, Jonathan Arturo
Gillanders, Florencia
Rubert, Aldo Alberto
Jares, Elizabeth Andrea
Jovin, Thomas M.
Fonticelli, Mariano Hernán
ESIPT and FRET probes for monitoring nanoparticle polymer coating stability
topic_facet Química
Tem
Nanoparticles
Coalescence
Thiols
description Coating strategies of inorganic nanoparticles (NPs) can provide properties unavailable to the NP core alone, such as targeting, specific sensing, and increased biocompatibility. Non-covalent amphiphilic NP capping polymers function via hydrophobic interactions with surface ligands and are extensively used to transfer NPs to aqueous media. For applications of coated NPs as actuators (sensors, markers, or for drug delivery) in a complex environment, such as biological systems, it is important to achieve a deep understanding of the factors affecting coating stability and behavior. We have designed a system that tests the coating stability of amphiphilic polymers through a simple fluorescent readout using either polarity sensing ESIPT (excited state intramolecular proton transfer) dyes or NP FRET (Förster resonance energy transfer). The stability of the coating was determined in response to changes in polarity, pH and ionic strength in the medium. Using the ESIPT system we observed linear changes in signal up to ∼20-25% v/v of co-solvent addition, constituting a break point. Based on such data, we propose a model for coating instability and the important adjustable parameters, such as the electrical charge distribution. FRET data provided confirmatory evidence for the model. The ESIPT dyes and FRET based methods represent new, simple tools for testing NP coating stability in complex environments.
format Articulo
Preprint
author Azcárate, Julio César
Díaz, Sebastián A.
Fauerbach, Jonathan Arturo
Gillanders, Florencia
Rubert, Aldo Alberto
Jares, Elizabeth Andrea
Jovin, Thomas M.
Fonticelli, Mariano Hernán
author_facet Azcárate, Julio César
Díaz, Sebastián A.
Fauerbach, Jonathan Arturo
Gillanders, Florencia
Rubert, Aldo Alberto
Jares, Elizabeth Andrea
Jovin, Thomas M.
Fonticelli, Mariano Hernán
author_sort Azcárate, Julio César
title ESIPT and FRET probes for monitoring nanoparticle polymer coating stability
title_short ESIPT and FRET probes for monitoring nanoparticle polymer coating stability
title_full ESIPT and FRET probes for monitoring nanoparticle polymer coating stability
title_fullStr ESIPT and FRET probes for monitoring nanoparticle polymer coating stability
title_full_unstemmed ESIPT and FRET probes for monitoring nanoparticle polymer coating stability
title_sort esipt and fret probes for monitoring nanoparticle polymer coating stability
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/99444
https://ri.conicet.gov.ar/11336/63229
https://pubs.rsc.org/en/Content/ArticleLanding/2017/NR/C7NR01787A
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bdutipo_str Repositorios
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