Possible radioprotective effect of folic acid supplementation on low dose ionizing radiation-induced genomic instability <i>in vitro</i>

Ionizing radiation (IR) induces DNA damage through production of single and double-strand breaks and reactive oxygen species (ROS). Folic acid (FA) prevents radiation-induced DNA damage by modification of DNA synthesis and/or repair and as a radical scavenger. We hypothesized that <i>in vitro&...

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Autores principales: Padula, Gisel, Ponzinibbio, María Virginia, Seoane, Analía Isabel
Formato: Articulo
Lenguaje:Inglés
Publicado: 2016
Materias:
Veterinaria
Ciencias Naturales
Radiation effects
DNA damage
Apoptosis
CHO cells
Chromosome instability
Occupational/enviromental hazards
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/98641
https://ri.conicet.gov.ar/11336/53861
http://nopr.niscair.res.in/handle/123456789/35146
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-98641
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Veterinaria
Ciencias Naturales
Radiation effects
DNA damage
Apoptosis
CHO cells
Chromosome instability
Occupational/enviromental hazards
spellingShingle Veterinaria
Ciencias Naturales
Radiation effects
DNA damage
Apoptosis
CHO cells
Chromosome instability
Occupational/enviromental hazards
Padula, Gisel
Ponzinibbio, María Virginia
Seoane, Analía Isabel
Possible radioprotective effect of folic acid supplementation on low dose ionizing radiation-induced genomic instability <i>in vitro</i>
topic_facet Veterinaria
Ciencias Naturales
Radiation effects
DNA damage
Apoptosis
CHO cells
Chromosome instability
Occupational/enviromental hazards
description Ionizing radiation (IR) induces DNA damage through production of single and double-strand breaks and reactive oxygen species (ROS). Folic acid (FA) prevents radiation-induced DNA damage by modification of DNA synthesis and/or repair and as a radical scavenger. We hypothesized that <i>in vitro</i> supplementation with FA will decrease the sensitivity of cells to genetic damage induced by low dose of ionizing radiation. Annexin V, comet and micronucleus assays were performed in cultured CHO cells. After 7 days of pre-treatment with 0, 100, 200 or 300 nM FA, cultures were exposed to radiation (100 mSv). Two un-irradiated controls were executed (0 and 100 nM FA). Data were statistically analyzed with X<sup>2</sup>-test and linear regression analysis (P < 0.05). We observed a significantly decreased frequency of apoptotic cells with the increasing FA concentration (P < 0.05). The same trend was observed when analyzing DNA damage and chromosomal instability (P < 0.05 for 300 nM). Only micronuclei frequencies showed significant differences for linear regression analysis (R2 = 94.04; P < 0.01). Our results have demonstrated the radioprotective effect of folic acid supplementation on low dose ionizing radiation-induced genomic instability <i>in vitro</i>; folate status should be taken into account when studying the effect of low dose radiation in environmental or occupational exposure.
format Articulo
Articulo
author Padula, Gisel
Ponzinibbio, María Virginia
Seoane, Analía Isabel
author_facet Padula, Gisel
Ponzinibbio, María Virginia
Seoane, Analía Isabel
author_sort Padula, Gisel
title Possible radioprotective effect of folic acid supplementation on low dose ionizing radiation-induced genomic instability <i>in vitro</i>
title_short Possible radioprotective effect of folic acid supplementation on low dose ionizing radiation-induced genomic instability <i>in vitro</i>
title_full Possible radioprotective effect of folic acid supplementation on low dose ionizing radiation-induced genomic instability <i>in vitro</i>
title_fullStr Possible radioprotective effect of folic acid supplementation on low dose ionizing radiation-induced genomic instability <i>in vitro</i>
title_full_unstemmed Possible radioprotective effect of folic acid supplementation on low dose ionizing radiation-induced genomic instability <i>in vitro</i>
title_sort possible radioprotective effect of folic acid supplementation on low dose ionizing radiation-induced genomic instability <i>in vitro</i>
publishDate 2016
url http://sedici.unlp.edu.ar/handle/10915/98641
https://ri.conicet.gov.ar/11336/53861
http://nopr.niscair.res.in/handle/123456789/35146
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