Proteolytic properties of <i>Funastrum clausum</i> latex

As part of a screening of latex endopeptidases from plants growing in Argentina, the presence of proteolytic activity in the latex of Funastrum clausum stems is reported. The proteases present in the crude extract showed the main characteristics of the cysteine proteolytic class, i.e. optimum pH at...

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Autores principales: Morcelle del Valle, Susana Raquel, Caffini, Néstor Oscar, Priolo de Lufrano, Nora Silvia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2004
Materias:
Biología
<i>Funastrum clausum</i>
Latex
Thiol proteases
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/97750
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-97750
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
<i>Funastrum clausum</i>
Latex
Thiol proteases
spellingShingle Biología
<i>Funastrum clausum</i>
Latex
Thiol proteases
Morcelle del Valle, Susana Raquel
Caffini, Néstor Oscar
Priolo de Lufrano, Nora Silvia
Proteolytic properties of <i>Funastrum clausum</i> latex
topic_facet Biología
<i>Funastrum clausum</i>
Latex
Thiol proteases
description As part of a screening of latex endopeptidases from plants growing in Argentina, the presence of proteolytic activity in the latex of Funastrum clausum stems is reported. The proteases present in the crude extract showed the main characteristics of the cysteine proteolytic class, i.e. optimum pH at alkaline range, isoelectric point (pI) higher than 9.0, and inhibition of proteolytic activity by thiol blocking reagents. A remarkable thermal stability was also evident in the crude extract. Endosterolytic preference tried on p-nitrophenyl esters of N-a-carbobenzoxy-L-amino acids was higher for the alanine, asparagine and tyrosine derivatives. Preliminary peptidase purification by two-step ionic exchange showed the presence of two proteolytic fractions with molecular masses of approximately 24.0 kDa according to SDS-PAGE.
format Articulo
Articulo
author Morcelle del Valle, Susana Raquel
Caffini, Néstor Oscar
Priolo de Lufrano, Nora Silvia
author_facet Morcelle del Valle, Susana Raquel
Caffini, Néstor Oscar
Priolo de Lufrano, Nora Silvia
author_sort Morcelle del Valle, Susana Raquel
title Proteolytic properties of <i>Funastrum clausum</i> latex
title_short Proteolytic properties of <i>Funastrum clausum</i> latex
title_full Proteolytic properties of <i>Funastrum clausum</i> latex
title_fullStr Proteolytic properties of <i>Funastrum clausum</i> latex
title_full_unstemmed Proteolytic properties of <i>Funastrum clausum</i> latex
title_sort proteolytic properties of <i>funastrum clausum</i> latex
publishDate 2004
url http://sedici.unlp.edu.ar/handle/10915/97750
work_keys_str_mv AT morcelledelvallesusanaraquel proteolyticpropertiesofifunastrumclausumilatex
AT caffininestoroscar proteolyticpropertiesofifunastrumclausumilatex
AT priolodelufranonorasilvia proteolyticpropertiesofifunastrumclausumilatex
bdutipo_str Repositorios
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