Cardioprotection of benzolamide in a regional ischemia model: Role of eNOS/NO
Background: Recent studies from our laboratory show the cardioprotective action of benzolamide (BZ, carbonic anhydrase inhibitor) against ischemia-reperfusion injury. However, the mechanisms involved have not been fully elucidated. Objective: To examine the participation of the endothelial nitric ox...
| Autores principales: | , , , , , |
|---|---|
| Formato: | Articulo Preprint |
| Lenguaje: | Inglés |
| Publicado: |
2018
|
| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/97107 https://ri.conicet.gov.ar/11336/92259 https://linkinghub.elsevier.com/retrieve/pii/S0014480018302673 |
| Aporte de: |
| id |
I19-R120-10915-97107 |
|---|---|
| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Médicas Benzolamide Carbonic anhydrase inhibition eNOS/NO Coronary artery occlusion |
| spellingShingle |
Ciencias Médicas Benzolamide Carbonic anhydrase inhibition eNOS/NO Coronary artery occlusion González Arbeláez, Luisa Fernanda Ciocci Pardo, Alejandro Swenson, Eric R. Álvarez, Bernardo Víctor Mosca, Susana María Fantinelli, Juliana Catalina Cardioprotection of benzolamide in a regional ischemia model: Role of eNOS/NO |
| topic_facet |
Ciencias Médicas Benzolamide Carbonic anhydrase inhibition eNOS/NO Coronary artery occlusion |
| description |
Background: Recent studies from our laboratory show the cardioprotective action of benzolamide (BZ, carbonic anhydrase inhibitor) against ischemia-reperfusion injury. However, the mechanisms involved have not been fully elucidated. Objective: To examine the participation of the endothelial nitric oxide synthase (eNOS)/nitric oxide (NO) in the effects of BZ in a model of regional ischemia.Methods: Isolated rat hearts perfused by Langendorff technique were submitted to 40 min of coronary artery occlusion followed by 60 min of reperfusion (IC). Other hearts received BZ during the first 10 min of reperfusion in absence or presence of L-NAME, NOS inhibitor. The infarct size (IS) and the post-ischemic recovery of myocardial function were measured. Oxidative/nitrosative damage were assessed by reduced glutathione (GSH) content, thiobarbituric acid reactivesubstances (TBARS) and 3-nitrotyrosine levels. The expression of phosphorylated forms of Akt, p38MAPK and eNOS, and the concentration of inducible nitric oxide synthase (iNOS) were also determined. Results: BZ significantly decreased IS (6.2 ± 0.5% vs. 34 ± 4%), improved postischemic contractility, preserved GSH levels and diminished TBARS and 3-nitrotyrosine. In IC hearts, P-Akt, P-p38MAPK and P-eNOS decreased and iNOS increased. After BZ addition the levels of P-kinases and P-eNOS increased and iNOS decreased. Except for P-Akt, P-p38MAPK and iNOS, the effects of BZ were abolished by L-NAME. Conclusions: Our data demonstrate that the treatment with BZ at the onset of reperfusion was effective to reduce cell death, contractile dysfunction and oxidative/nitrosative damage produced by coronary artery occlusion. These BZmediated beneficial actions appear mediated by eNOS/NO-dependent pathways. |
| format |
Articulo Preprint |
| author |
González Arbeláez, Luisa Fernanda Ciocci Pardo, Alejandro Swenson, Eric R. Álvarez, Bernardo Víctor Mosca, Susana María Fantinelli, Juliana Catalina |
| author_facet |
González Arbeláez, Luisa Fernanda Ciocci Pardo, Alejandro Swenson, Eric R. Álvarez, Bernardo Víctor Mosca, Susana María Fantinelli, Juliana Catalina |
| author_sort |
González Arbeláez, Luisa Fernanda |
| title |
Cardioprotection of benzolamide in a regional ischemia model: Role of eNOS/NO |
| title_short |
Cardioprotection of benzolamide in a regional ischemia model: Role of eNOS/NO |
| title_full |
Cardioprotection of benzolamide in a regional ischemia model: Role of eNOS/NO |
| title_fullStr |
Cardioprotection of benzolamide in a regional ischemia model: Role of eNOS/NO |
| title_full_unstemmed |
Cardioprotection of benzolamide in a regional ischemia model: Role of eNOS/NO |
| title_sort |
cardioprotection of benzolamide in a regional ischemia model: role of enos/no |
| publishDate |
2018 |
| url |
http://sedici.unlp.edu.ar/handle/10915/97107 https://ri.conicet.gov.ar/11336/92259 https://linkinghub.elsevier.com/retrieve/pii/S0014480018302673 |
| work_keys_str_mv |
AT gonzalezarbelaezluisafernanda cardioprotectionofbenzolamideinaregionalischemiamodelroleofenosno AT cioccipardoalejandro cardioprotectionofbenzolamideinaregionalischemiamodelroleofenosno AT swensonericr cardioprotectionofbenzolamideinaregionalischemiamodelroleofenosno AT alvarezbernardovictor cardioprotectionofbenzolamideinaregionalischemiamodelroleofenosno AT moscasusanamaria cardioprotectionofbenzolamideinaregionalischemiamodelroleofenosno AT fantinellijulianacatalina cardioprotectionofbenzolamideinaregionalischemiamodelroleofenosno |
| bdutipo_str |
Repositorios |
| _version_ |
1764820492369264641 |