Outer membrane protein OmpQ of <i>Bordetella bronchiseptica</i> is required for mature biofilm formation

Bordetella bronchiseptica, an aerobic Gram-negative bacterium, is capable of colonizing the respiratory tract of diverse animals and chronically persists inside the hosts by forming biofilm. Most known virulence factors in Bordetella species are regulated by the BvgAS two-component transduction syst...

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Autores principales: Cattelan, Natalia, Villalba, María Inés, Parisi, Gustavo Daniel, Arnal, Laura, Serra, Diego Omar, Aguilar, Orlando Mario, Yantorno, Osvaldo Miguel
Formato: Articulo
Lenguaje:Inglés
Publicado: 2016
Materias:
Biología
Ciencias Naturales
Biofilm
Bordetella bronchiseptica
Ompq
Pertussis
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/96595
https://ri.conicet.gov.ar/11336/66425
http://mic.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000224
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-96595
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Ciencias Naturales
Biofilm
Bordetella bronchiseptica
Ompq
Pertussis
spellingShingle Biología
Ciencias Naturales
Biofilm
Bordetella bronchiseptica
Ompq
Pertussis
Cattelan, Natalia
Villalba, María Inés
Parisi, Gustavo Daniel
Arnal, Laura
Serra, Diego Omar
Aguilar, Orlando Mario
Yantorno, Osvaldo Miguel
Outer membrane protein OmpQ of <i>Bordetella bronchiseptica</i> is required for mature biofilm formation
topic_facet Biología
Ciencias Naturales
Biofilm
Bordetella bronchiseptica
Ompq
Pertussis
description Bordetella bronchiseptica, an aerobic Gram-negative bacterium, is capable of colonizing the respiratory tract of diverse animals and chronically persists inside the hosts by forming biofilm. Most known virulence factors in Bordetella species are regulated by the BvgAS two-component transduction system. The Bvg-activated proteins play a critical role during host infection. OmpQ is an outer membrane porin protein which is expressed under BvgAS control. Here, we studied the contribution of OmpQ to the biofilm formation process by B. bronchiseptica. We found that the lack of expression of OmpQ did not affect the growth kinetics and final biomass of B. bronchiseptica under planktonic growth conditions. The DompQ mutant strain displayed no differences in attachment level and in early steps of biofilm formation. However, deletion of the ompQ gene attenuated the ability of B. bronchiseptica to form a mature biofilm. Analysis of ompQ gene expression during the biofilm formation process by B. bronchiseptica showed a dynamic expression pattern, with an increase of biofilm culture at 48 h. Moreover, we demonstrated that the addition of serum anti-OmpQ had the potential to reduce the biofilm biomass formation in a dose-dependent manner. In conclusion, we showed for the first time, to the best of our knowledge, evidence of the contribution of OmpQ to a process of importance for B. bronchiseptica pathobiology. Our results indicate that OmpQ plays a role during the biofilm development process, particularly at later stages of development, and that this porin could be a potential target for strategies of biofilm formation inhibition.
format Articulo
Articulo
author Cattelan, Natalia
Villalba, María Inés
Parisi, Gustavo Daniel
Arnal, Laura
Serra, Diego Omar
Aguilar, Orlando Mario
Yantorno, Osvaldo Miguel
author_facet Cattelan, Natalia
Villalba, María Inés
Parisi, Gustavo Daniel
Arnal, Laura
Serra, Diego Omar
Aguilar, Orlando Mario
Yantorno, Osvaldo Miguel
author_sort Cattelan, Natalia
title Outer membrane protein OmpQ of <i>Bordetella bronchiseptica</i> is required for mature biofilm formation
title_short Outer membrane protein OmpQ of <i>Bordetella bronchiseptica</i> is required for mature biofilm formation
title_full Outer membrane protein OmpQ of <i>Bordetella bronchiseptica</i> is required for mature biofilm formation
title_fullStr Outer membrane protein OmpQ of <i>Bordetella bronchiseptica</i> is required for mature biofilm formation
title_full_unstemmed Outer membrane protein OmpQ of <i>Bordetella bronchiseptica</i> is required for mature biofilm formation
title_sort outer membrane protein ompq of <i>bordetella bronchiseptica</i> is required for mature biofilm formation
publishDate 2016
url http://sedici.unlp.edu.ar/handle/10915/96595
https://ri.conicet.gov.ar/11336/66425
http://mic.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000224
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