Sequence-dependent catalytic regulation of the SpoIIIE motor activity ensures directionality of DNA translocation

Transport of cellular cargo by molecular motors requires directionality to ensure proper biological functioning. During sporulation in Bacillus subtilis, directionality of chromosome transport is mediated by the interaction between the membrane-bound DNA translocase SpoIIIE and specific octameric se...

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Detalles Bibliográficos
Autores principales: Chara, Osvaldo, Borges, Augusto, Milhiet, Pierre Emmanuel, Nöllmann, Marcelo, Cattoni, Diego I.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2018
Materias:
Biología
DNA translocation
Sporulation
Monte Carlo modelling
Chromosome transport
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/96525
https://ri.conicet.gov.ar/11336/87917
https://www.nature.com/articles/s41598-018-23400-8
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-96525
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
DNA translocation
Sporulation
Monte Carlo modelling
Chromosome transport
spellingShingle Biología
DNA translocation
Sporulation
Monte Carlo modelling
Chromosome transport
Chara, Osvaldo
Borges, Augusto
Milhiet, Pierre Emmanuel
Nöllmann, Marcelo
Cattoni, Diego I.
Sequence-dependent catalytic regulation of the SpoIIIE motor activity ensures directionality of DNA translocation
topic_facet Biología
DNA translocation
Sporulation
Monte Carlo modelling
Chromosome transport
description Transport of cellular cargo by molecular motors requires directionality to ensure proper biological functioning. During sporulation in Bacillus subtilis, directionality of chromosome transport is mediated by the interaction between the membrane-bound DNA translocase SpoIIIE and specific octameric sequences (SRS). Whether SRS regulate directionality by recruiting and orienting SpoIIIE or by simply catalyzing its translocation activity is still unclear. By using atomic force microscopy and single-round fast kinetics translocation assays we determined the localization and dynamics of diffusing and translocating SpoIIIE complexes on DNA with or without SRS. Our findings combined with mathematical modelling revealed that SpoIIIE directionality is not regulated by protein recruitment to SRS but rather by a fine-tuned balance among the rates governing SpoIIIE-DNA interactions and the probability of starting translocation modulated by SRS. Additionally, we found that SpoIIIE can start translocation from non-specific DNA, providing an alternative active search mechanism for SRS located beyond the exploratory length defined by 1D diffusion. These findings are relevant in vivo in the context of chromosome transport through an open channel, where SpoIIIE can rapidly explore DNA while directionality is modulated by the probability of translocation initiation upon interaction with SRS versus non-specific DNA.
format Articulo
Articulo
author Chara, Osvaldo
Borges, Augusto
Milhiet, Pierre Emmanuel
Nöllmann, Marcelo
Cattoni, Diego I.
author_facet Chara, Osvaldo
Borges, Augusto
Milhiet, Pierre Emmanuel
Nöllmann, Marcelo
Cattoni, Diego I.
author_sort Chara, Osvaldo
title Sequence-dependent catalytic regulation of the SpoIIIE motor activity ensures directionality of DNA translocation
title_short Sequence-dependent catalytic regulation of the SpoIIIE motor activity ensures directionality of DNA translocation
title_full Sequence-dependent catalytic regulation of the SpoIIIE motor activity ensures directionality of DNA translocation
title_fullStr Sequence-dependent catalytic regulation of the SpoIIIE motor activity ensures directionality of DNA translocation
title_full_unstemmed Sequence-dependent catalytic regulation of the SpoIIIE motor activity ensures directionality of DNA translocation
title_sort sequence-dependent catalytic regulation of the spoiiie motor activity ensures directionality of dna translocation
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/96525
https://ri.conicet.gov.ar/11336/87917
https://www.nature.com/articles/s41598-018-23400-8
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AT milhietpierreemmanuel sequencedependentcatalyticregulationofthespoiiiemotoractivityensuresdirectionalityofdnatranslocation
AT nollmannmarcelo sequencedependentcatalyticregulationofthespoiiiemotoractivityensuresdirectionalityofdnatranslocation
AT cattonidiegoi sequencedependentcatalyticregulationofthespoiiiemotoractivityensuresdirectionalityofdnatranslocation
bdutipo_str Repositorios
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