Substrate engagement of integrins α5 β1 and αv β3 is necessary, but not sufficient, for high directional persistence in migration on fibronectin

The interplay between specific integrin-mediated matrix adhesion and directional persistence in cell migration is not well understood. Here, we characterized fibroblast adhesion and migration on the extracellular matrix glycoproteins fibronectin and vitronectin, focusing on the role of α5 β1 and αv...

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Detalles Bibliográficos
Autores principales: Missirlis, Dimitris, Haraszti, Tamás, Scheele, Catharina v. C., Wiegand, Tina, Díaz, Carolina, Neubauer, Stefanie, Rechenmacher, Florian, Kessler, Horst, Spatz, Joachim P.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2016
Materias:
Química
Focal adhesion
Integrin signaling
Fibronectin
Migration
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/96483
https://ri.conicet.gov.ar/11336/49069
https://www.nature.com/articles/srep23258
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-96483
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Focal adhesion
Integrin signaling
Fibronectin
Migration
spellingShingle Química
Focal adhesion
Integrin signaling
Fibronectin
Migration
Missirlis, Dimitris
Haraszti, Tamás
Scheele, Catharina v. C.
Wiegand, Tina
Díaz, Carolina
Neubauer, Stefanie
Rechenmacher, Florian
Kessler, Horst
Spatz, Joachim P.
Substrate engagement of integrins α5 β1 and αv β3 is necessary, but not sufficient, for high directional persistence in migration on fibronectin
topic_facet Química
Focal adhesion
Integrin signaling
Fibronectin
Migration
description The interplay between specific integrin-mediated matrix adhesion and directional persistence in cell migration is not well understood. Here, we characterized fibroblast adhesion and migration on the extracellular matrix glycoproteins fibronectin and vitronectin, focusing on the role of α5 β1 and αv β3 integrins. Fibroblasts manifested high directional persistence in migration on fibronectin-, but not vitronectin-coated substrates, in a ligand density-dependent manner. Fibronectin stimulated α5 β1-dependent organization of the actin cytoskeleton into oriented, ventral stress fibers, and assembly of dynamic, polarized protrusions, characterized as regions free of stress fibers and rich in nascent adhesions at their edge. Such protrusions correlated with persistent, local leading edge advancement, but were not sufficient, nor necessary for directional migration over longer times. Selective blocking of αv β3 or α5 β1 integrins using small molecule integrin antagonists reduced directional persistence on fibronectin, indicating integrin cooperativity in maintaining directionality. On the other hand, patterned substrates, designed to selectively engage either integrin, or their combination, were not sufficient to establish directional migration. Overall, our study demonstrates adhesive coating-dependent regulation of directional persistence in fibroblast migration and challenges the generality of the previously suggested role of β1 and β3 integrins in directional migration.
format Articulo
Articulo
author Missirlis, Dimitris
Haraszti, Tamás
Scheele, Catharina v. C.
Wiegand, Tina
Díaz, Carolina
Neubauer, Stefanie
Rechenmacher, Florian
Kessler, Horst
Spatz, Joachim P.
author_facet Missirlis, Dimitris
Haraszti, Tamás
Scheele, Catharina v. C.
Wiegand, Tina
Díaz, Carolina
Neubauer, Stefanie
Rechenmacher, Florian
Kessler, Horst
Spatz, Joachim P.
author_sort Missirlis, Dimitris
title Substrate engagement of integrins α5 β1 and αv β3 is necessary, but not sufficient, for high directional persistence in migration on fibronectin
title_short Substrate engagement of integrins α5 β1 and αv β3 is necessary, but not sufficient, for high directional persistence in migration on fibronectin
title_full Substrate engagement of integrins α5 β1 and αv β3 is necessary, but not sufficient, for high directional persistence in migration on fibronectin
title_fullStr Substrate engagement of integrins α5 β1 and αv β3 is necessary, but not sufficient, for high directional persistence in migration on fibronectin
title_full_unstemmed Substrate engagement of integrins α5 β1 and αv β3 is necessary, but not sufficient, for high directional persistence in migration on fibronectin
title_sort substrate engagement of integrins α5 β1 and αv β3 is necessary, but not sufficient, for high directional persistence in migration on fibronectin
publishDate 2016
url http://sedici.unlp.edu.ar/handle/10915/96483
https://ri.conicet.gov.ar/11336/49069
https://www.nature.com/articles/srep23258
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