Genetic diversity among viruses associated with sugarcane mosaic disease in Tucumán, Argentina

Sugarcane leaves with mosaic symptoms were collected in 2006--07 in Tucumán (Argentina) and analyzed by reverse-transcriptase polymerase chain reaction (RT-PCR) restriction fragment length polymorphism (RFLP) and sequencing of a fragment of the Sugarcane mosaic virus (SCMV) and Sorghum mosaic virus...

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Detalles Bibliográficos
Autores principales: Perera, María Francisca, Filippone, María Paula, Ramallo, C. J., Cuenya, María Inés, García, María Laura, Ploper, Leonardo Daniel, Castagnaro, Atilio Pedro
Formato: Articulo
Lenguaje:Inglés
Publicado: 2009
Materias:
Biología
Coat protein gene
Potyviridae
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/95908
https://ri.conicet.gov.ar/11336/80692
https://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-99-1-0038
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-95908
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Coat protein gene
Potyviridae
spellingShingle Biología
Coat protein gene
Potyviridae
Perera, María Francisca
Filippone, María Paula
Ramallo, C. J.
Cuenya, María Inés
García, María Laura
Ploper, Leonardo Daniel
Castagnaro, Atilio Pedro
Genetic diversity among viruses associated with sugarcane mosaic disease in Tucumán, Argentina
topic_facet Biología
Coat protein gene
Potyviridae
description Sugarcane leaves with mosaic symptoms were collected in 2006--07 in Tucumán (Argentina) and analyzed by reverse-transcriptase polymerase chain reaction (RT-PCR) restriction fragment length polymorphism (RFLP) and sequencing of a fragment of the Sugarcane mosaic virus (SCMV) and Sorghum mosaic virus (SrMV) coat protein (CP) genes. SCMV was detected in 96.6% of samples, with 41% showing the RFLP profile consistent with strain E. The remaining samples produced eight different profiles that did not match other known strains. SCMV distribution seemed to be more related to sugarcane genotype than to geographical origin, and sequence analyses of CP genes showed a greater genetic diversity compared with other studies. SrMV was detected in 63.2% of samples and most of these were also infected by SCMV, indicating that, unlike other countries and other Argentinean provinces, where high levels of co-infection are infrequent, co-existence is common in Tucumán. RFLP analysis showed the presence of SrMV strains M (68%) and I (14%), while co-infection between M and H strains was present in 18% of samples. Other SCMV subgroup members and the Sugarcane streak mosaic virus (SCSMV) were not detected. Our results also showed that sequencing is currently the only reliable method to assess SCMV and SrMV genetic diversity, because RT-PCR-RFLP may not be sufficiently discriminating.
format Articulo
Articulo
author Perera, María Francisca
Filippone, María Paula
Ramallo, C. J.
Cuenya, María Inés
García, María Laura
Ploper, Leonardo Daniel
Castagnaro, Atilio Pedro
author_facet Perera, María Francisca
Filippone, María Paula
Ramallo, C. J.
Cuenya, María Inés
García, María Laura
Ploper, Leonardo Daniel
Castagnaro, Atilio Pedro
author_sort Perera, María Francisca
title Genetic diversity among viruses associated with sugarcane mosaic disease in Tucumán, Argentina
title_short Genetic diversity among viruses associated with sugarcane mosaic disease in Tucumán, Argentina
title_full Genetic diversity among viruses associated with sugarcane mosaic disease in Tucumán, Argentina
title_fullStr Genetic diversity among viruses associated with sugarcane mosaic disease in Tucumán, Argentina
title_full_unstemmed Genetic diversity among viruses associated with sugarcane mosaic disease in Tucumán, Argentina
title_sort genetic diversity among viruses associated with sugarcane mosaic disease in tucumán, argentina
publishDate 2009
url http://sedici.unlp.edu.ar/handle/10915/95908
https://ri.conicet.gov.ar/11336/80692
https://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-99-1-0038
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