Antifungal Activity of Arginine-Based Surfactants

Background: Amino acid based surfactants constitute an important class of surface active biomolecules showing remarkable biocompatible properties. Antimicrobial activity is one of the most remarkable biological properties of this kind of surfactants, which have been widely studied against a broad sp...

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Autores principales: Fait, María Elisa, S. da Costa, Helen P., Freitas, Cleverson D. T., Bakás, Laura Susana, Morcelle del Valle, Susana Raquel
Formato: Articulo
Lenguaje:Inglés
Publicado: 2018
Materias:
Biología
Arginine-based surfactants
Antifungal activity
Phytopathogenic fungi
Dermatophytes
Membrane damage
ROS production
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/92364
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-92364
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Arginine-based surfactants
Antifungal activity
Phytopathogenic fungi
Dermatophytes
Membrane damage
ROS production
spellingShingle Biología
Arginine-based surfactants
Antifungal activity
Phytopathogenic fungi
Dermatophytes
Membrane damage
ROS production
Fait, María Elisa
S. da Costa, Helen P.
Freitas, Cleverson D. T.
Bakás, Laura Susana
Morcelle del Valle, Susana Raquel
Antifungal Activity of Arginine-Based Surfactants
topic_facet Biología
Arginine-based surfactants
Antifungal activity
Phytopathogenic fungi
Dermatophytes
Membrane damage
ROS production
description Background: Amino acid based surfactants constitute an important class of surface active biomolecules showing remarkable biocompatible properties. Antimicrobial activity is one of the most remarkable biological properties of this kind of surfactants, which have been widely studied against a broad spectrum of microorganisms. However, the antifungal activity of this kind of compound has been less well investigated. The aim of this work is the study of the antifungal activity of two novel argininebased surfactants (N -benzoyl-arginine decylamide, Bz-Arg-NHC10 and N -benzoyl-arginine dodecylamide, Bz-Arg-NHC12), obtained by an enzymatic strategy, against phytopathogenic filamentous fungi and dermatophyte strains. Methods: Four phytopathogenic fungi (Fusarium oxysporum, Fusarium solani, Colletotrichum gloeosporioides and Colletotrichum lindemuthianum) and two human pathogenic fungi (dermatophytes Trichophyton rubrum and Trichophyton mentagrophytes) were tested. Inhibition of vegetative growth and conidia germination was investigated for the phytopathogenic fungi. In order to elucidate the possible mechanism of biocide action, membrane integrity, as well as the production of reactive oxygen species (ROS) were evaluated. Additionally, the inhibition of germination of dermatophyte microconidia due to both arginine-based surfactants was studied. Minimum inhibitory concentration, as well as the concentration that inhibits 50% of germination were determined for both compounds and both fungal strains. Results: For the vegetative growth of phytopathogenic fungi, the most potent arginine-based compound was Bz-Arg-NHC10. All the tested compounds interfered with the conidia development of the studied species. Investigation of the possible mechanism of toxicity towards phytopathogenic fungi indicated direct damage of the plasma membrane and production of ROS. For the two strains of dermatophyte fungi tested, all the proved compounds showed similar fungistatic efficacy. Conclusion: Bz-Arg-NHC10 and Bz-Arg-NHC12 were demonstrated to have broad biocidal ability against the proliferative vegetative form and the asexual reproductive conidia. Results suggest that both membrane permeabilization and induction of oxidative stress are part of the antifungal mechanisms involved in the interruption of normal conidia development by Bz-Arg-NHCn, leading to cell death.
format Articulo
Articulo
author Fait, María Elisa
S. da Costa, Helen P.
Freitas, Cleverson D. T.
Bakás, Laura Susana
Morcelle del Valle, Susana Raquel
author_facet Fait, María Elisa
S. da Costa, Helen P.
Freitas, Cleverson D. T.
Bakás, Laura Susana
Morcelle del Valle, Susana Raquel
author_sort Fait, María Elisa
title Antifungal Activity of Arginine-Based Surfactants
title_short Antifungal Activity of Arginine-Based Surfactants
title_full Antifungal Activity of Arginine-Based Surfactants
title_fullStr Antifungal Activity of Arginine-Based Surfactants
title_full_unstemmed Antifungal Activity of Arginine-Based Surfactants
title_sort antifungal activity of arginine-based surfactants
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/92364
work_keys_str_mv AT faitmariaelisa antifungalactivityofargininebasedsurfactants
AT sdacostahelenp antifungalactivityofargininebasedsurfactants
AT freitascleversondt antifungalactivityofargininebasedsurfactants
AT bakaslaurasusana antifungalactivityofargininebasedsurfactants
AT morcelledelvallesusanaraquel antifungalactivityofargininebasedsurfactants
bdutipo_str Repositorios
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