Genetic diversity of <i>Mycobacterium avium</i> complex strains isolated in Argentina by MIRU-VNTR

<i>Mycobacterium avium</i> sp. <i>avium</i> (MAA), <i>M. avium</i> sp. <i>hominissuis</i> (MAH), and <i>M. avium</i> sp. <i>paratuberculosis</i> (MAP) are the main members of the <i>M. avium</i> complex (MAC) causing...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Imperiale, Belén Rocío, Moyano, Roberto Damián, Di Giulio, A. B., Romero, Magalí Andrea, Alvarado Pinedo, María Fiorella, Santangelo, María de la Paz, Travería, Gabriel Eduardo, Morcillo, N. S., Romano, María Isabel
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Ciencias Veterinarias
Diversity
MIRU-VNTR
Mycobacterium avium complex
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/87667
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-87667
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Diversity
MIRU-VNTR
Mycobacterium avium complex
spellingShingle Ciencias Veterinarias
Diversity
MIRU-VNTR
Mycobacterium avium complex
Imperiale, Belén Rocío
Moyano, Roberto Damián
Di Giulio, A. B.
Romero, Magalí Andrea
Alvarado Pinedo, María Fiorella
Santangelo, María de la Paz
Travería, Gabriel Eduardo
Morcillo, N. S.
Romano, María Isabel
Genetic diversity of <i>Mycobacterium avium</i> complex strains isolated in Argentina by MIRU-VNTR
topic_facet Ciencias Veterinarias
Diversity
MIRU-VNTR
Mycobacterium avium complex
description <i>Mycobacterium avium</i> sp. <i>avium</i> (MAA), <i>M. avium</i> sp. <i>hominissuis</i> (MAH), and <i>M. avium</i> sp. <i>paratuberculosis</i> (MAP) are the main members of the <i>M. avium</i> complex (MAC) causing diseases in several hosts. The aim of this study was to describe the genetic diversity of MAC isolated from different hosts. Twenty-six MAH and 61 MAP isolates were recovered from humans and cattle, respectively. GenoType CM® and IS1311-PCR were used to identify <i>Mycobacterium</i> species. The IS901-PCR was used to differentiate between MAH and MAA, while IS900-PCR was used to identify MAP. Genotyping was performed using a mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) scheme (loci: 292, X3, 25, 47, 3, 7, 10, 32) and patterns (INMV) were assigned according to the MAC-INMV database (http://mac-inmv.tours.inra.fr/). Twenty-two (22/26, 84·6%) MAH isolates were genotyped and 16 were grouped into the following, INMV 92, INMV 121, INMV 97, INMV 103, INMV 50, and INMV 40. The loci X3 and 25 showed the largest diversity (D: 0·5844), and the global discriminatory index (Hunter and Gaston discriminatory index, HGDI) was 0·9300. MAP (100%) isolates were grouped into INMV 1, INMV 2, INMV 11, INMV 8, and INMV 5. The HGDI was 0·6984 and loci 292 and 7 had the largest D (0·6980 and 0·5050). MAH presented a higher D when compared with MAP. The MIRU-VNTR was a useful tool to describe the genetic diversity of both MAH and MAP as well as to identify six new MAH patterns that were conveniently reported to the MAC-INMV database. It was also demonstrated that, in the geographical region studied, human MAC cases were produced by MAH as there was no MAA found among the human clinical samples.
format Articulo
Articulo
author Imperiale, Belén Rocío
Moyano, Roberto Damián
Di Giulio, A. B.
Romero, Magalí Andrea
Alvarado Pinedo, María Fiorella
Santangelo, María de la Paz
Travería, Gabriel Eduardo
Morcillo, N. S.
Romano, María Isabel
author_facet Imperiale, Belén Rocío
Moyano, Roberto Damián
Di Giulio, A. B.
Romero, Magalí Andrea
Alvarado Pinedo, María Fiorella
Santangelo, María de la Paz
Travería, Gabriel Eduardo
Morcillo, N. S.
Romano, María Isabel
author_sort Imperiale, Belén Rocío
title Genetic diversity of <i>Mycobacterium avium</i> complex strains isolated in Argentina by MIRU-VNTR
title_short Genetic diversity of <i>Mycobacterium avium</i> complex strains isolated in Argentina by MIRU-VNTR
title_full Genetic diversity of <i>Mycobacterium avium</i> complex strains isolated in Argentina by MIRU-VNTR
title_fullStr Genetic diversity of <i>Mycobacterium avium</i> complex strains isolated in Argentina by MIRU-VNTR
title_full_unstemmed Genetic diversity of <i>Mycobacterium avium</i> complex strains isolated in Argentina by MIRU-VNTR
title_sort genetic diversity of <i>mycobacterium avium</i> complex strains isolated in argentina by miru-vntr
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/87667
work_keys_str_mv AT imperialebelenrocio geneticdiversityofimycobacteriumaviumicomplexstrainsisolatedinargentinabymiruvntr
AT moyanorobertodamian geneticdiversityofimycobacteriumaviumicomplexstrainsisolatedinargentinabymiruvntr
AT digiulioab geneticdiversityofimycobacteriumaviumicomplexstrainsisolatedinargentinabymiruvntr
AT romeromagaliandrea geneticdiversityofimycobacteriumaviumicomplexstrainsisolatedinargentinabymiruvntr
AT alvaradopinedomariafiorella geneticdiversityofimycobacteriumaviumicomplexstrainsisolatedinargentinabymiruvntr
AT santangelomariadelapaz geneticdiversityofimycobacteriumaviumicomplexstrainsisolatedinargentinabymiruvntr
AT traveriagabrieleduardo geneticdiversityofimycobacteriumaviumicomplexstrainsisolatedinargentinabymiruvntr
AT morcillons geneticdiversityofimycobacteriumaviumicomplexstrainsisolatedinargentinabymiruvntr
AT romanomariaisabel geneticdiversityofimycobacteriumaviumicomplexstrainsisolatedinargentinabymiruvntr
bdutipo_str Repositorios
_version_ 1764820489271771136

Ejemplares similares