Membrane-bound glycerol dehydrogenase catalyzes oxidation of D-pentonates to 4-keto-D-pentonates, D-fructose to 5-keto-D-fructose, and D-psicose to 5-keto-D-psicose

A novel oxidation of D-pentonates to 4-keto-D-pentonates was analyzed with <i>Gluconobacter Thailandicus</i> NBRC 3258. D-Pentonate 4-dehydrogenase activity in the membrane fraction was readily inactivated by EDTA and it was reactivated by the addition of PQQ and Ca<sup>2+</sup&...

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Detalles Bibliográficos
Autores principales: Ano, Yoshitaka, Hours, Roque Alberto, Akakabe, Yoshihiko, Kataoka, Naoya, Yakushi, Toshiharu, Matsushita, Kazunobu, Adachi, Osao
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Ciencias Exactas
4-keto-D-arabonate production
4-keto-D-pentonate
Acetic acid bacteria
Glycerol dehydrogenase
Oxidative fermentation
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/87580
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-87580
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
4-keto-D-arabonate production
4-keto-D-pentonate
Acetic acid bacteria
Glycerol dehydrogenase
Oxidative fermentation
spellingShingle Ciencias Exactas
4-keto-D-arabonate production
4-keto-D-pentonate
Acetic acid bacteria
Glycerol dehydrogenase
Oxidative fermentation
Ano, Yoshitaka
Hours, Roque Alberto
Akakabe, Yoshihiko
Kataoka, Naoya
Yakushi, Toshiharu
Matsushita, Kazunobu
Adachi, Osao
Membrane-bound glycerol dehydrogenase catalyzes oxidation of D-pentonates to 4-keto-D-pentonates, D-fructose to 5-keto-D-fructose, and D-psicose to 5-keto-D-psicose
topic_facet Ciencias Exactas
4-keto-D-arabonate production
4-keto-D-pentonate
Acetic acid bacteria
Glycerol dehydrogenase
Oxidative fermentation
description A novel oxidation of D-pentonates to 4-keto-D-pentonates was analyzed with <i>Gluconobacter Thailandicus</i> NBRC 3258. D-Pentonate 4-dehydrogenase activity in the membrane fraction was readily inactivated by EDTA and it was reactivated by the addition of PQQ and Ca<sup>2+</sup>. D-Pentonate 4-dehydrogenase was purified to two different subunits, 80 and 14 kDa. The absorption spectrum of the purified enzyme showed no typical absorbance over the visible regions. The enzyme oxidized D-pentonates to 4-keto-D-pentonates at the optimum pH of 4.0. In addition, the enzyme oxidized D-fructose to 5-keto-D-fructose, D-psicose to 5-keto-D-psicose, including the other polyols such as, glycerol, D-ribitol, D-arabitol, and D-sorbitol. Thus, D-pentonate 4-dehydrogenase was found to be identical with glycerol dehydrogenase (GLDH), a major polyol dehydrogenase in <i>Gluconobacter</i> species. The reaction versatility of quinoprotein GLDH was notified in this study.
format Articulo
Articulo
author Ano, Yoshitaka
Hours, Roque Alberto
Akakabe, Yoshihiko
Kataoka, Naoya
Yakushi, Toshiharu
Matsushita, Kazunobu
Adachi, Osao
author_facet Ano, Yoshitaka
Hours, Roque Alberto
Akakabe, Yoshihiko
Kataoka, Naoya
Yakushi, Toshiharu
Matsushita, Kazunobu
Adachi, Osao
author_sort Ano, Yoshitaka
title Membrane-bound glycerol dehydrogenase catalyzes oxidation of D-pentonates to 4-keto-D-pentonates, D-fructose to 5-keto-D-fructose, and D-psicose to 5-keto-D-psicose
title_short Membrane-bound glycerol dehydrogenase catalyzes oxidation of D-pentonates to 4-keto-D-pentonates, D-fructose to 5-keto-D-fructose, and D-psicose to 5-keto-D-psicose
title_full Membrane-bound glycerol dehydrogenase catalyzes oxidation of D-pentonates to 4-keto-D-pentonates, D-fructose to 5-keto-D-fructose, and D-psicose to 5-keto-D-psicose
title_fullStr Membrane-bound glycerol dehydrogenase catalyzes oxidation of D-pentonates to 4-keto-D-pentonates, D-fructose to 5-keto-D-fructose, and D-psicose to 5-keto-D-psicose
title_full_unstemmed Membrane-bound glycerol dehydrogenase catalyzes oxidation of D-pentonates to 4-keto-D-pentonates, D-fructose to 5-keto-D-fructose, and D-psicose to 5-keto-D-psicose
title_sort membrane-bound glycerol dehydrogenase catalyzes oxidation of d-pentonates to 4-keto-d-pentonates, d-fructose to 5-keto-d-fructose, and d-psicose to 5-keto-d-psicose
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/87580
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