Bordetella parapertussis Circumvents Neutrophil Extracellular Bactericidal Mechanisms

B. parapertussis is a whooping cough etiological agent with the ability to evade the immune response induced by pertussis vaccines. We previously demonstrated that in the absence of opsonic antibodies B. parapertussis hampers phagocytosis by neutrophils and macrophages and, when phagocytosed, blocks...

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Detalles Bibliográficos
Autores principales: Gorgojo, Juan Pablo, Scharrig, Emilia, Gómez, Ricardo Martín, Harvill, Eric T., Rodríguez, María Eugenia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Biología
Bordetella pertussis
Neutrophils
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/87579
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-87579
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Bordetella pertussis
Neutrophils
spellingShingle Biología
Bordetella pertussis
Neutrophils
Gorgojo, Juan Pablo
Scharrig, Emilia
Gómez, Ricardo Martín
Harvill, Eric T.
Rodríguez, María Eugenia
Bordetella parapertussis Circumvents Neutrophil Extracellular Bactericidal Mechanisms
topic_facet Biología
Bordetella pertussis
Neutrophils
description B. parapertussis is a whooping cough etiological agent with the ability to evade the immune response induced by pertussis vaccines. We previously demonstrated that in the absence of opsonic antibodies B. parapertussis hampers phagocytosis by neutrophils and macrophages and, when phagocytosed, blocks intracellular killing by interfering with phagolysosomal fusion. But neutrophils can kill and/or immobilize extracellular bacteria through nonphagocytic mechanisms such as degranulation and neutrophil extracellular traps (NETs). In this study we demonstrated that B. parapertussis also has the ability to circumvent these two neutrophil extracellular bactericidal activities. The lack of neutrophil degranulation was found dependent on the O antigen that targets the bacteria to cell lipid rafts, eventually avoiding the fusion of nascent phagosomes with specific and azurophilic granules. IgG opsonization overcame this inhibition of neutrophil degranulation. We further observed that B. parapertussis did not induce NETs release in resting neutrophils and inhibited NETs formation in response to phorbol myristate acetate (PMA) stimulation by a mechanism dependent on adenylate cyclase toxin (CyaA)-mediated inhibition of reactive oxygen species (ROS) generation. Thus, B. parapertussis modulates neutrophil bactericidal activity through two different mechanisms, one related to the lack of proper NETs-inducer stimuli and the other one related to an active inhibitory mechanism. Together with previous results these data suggest that B. parapertussis has the ability to subvert the main neutrophil bactericidal functions, inhibiting efficient clearance in non-immune hosts.
format Articulo
Articulo
author Gorgojo, Juan Pablo
Scharrig, Emilia
Gómez, Ricardo Martín
Harvill, Eric T.
Rodríguez, María Eugenia
author_facet Gorgojo, Juan Pablo
Scharrig, Emilia
Gómez, Ricardo Martín
Harvill, Eric T.
Rodríguez, María Eugenia
author_sort Gorgojo, Juan Pablo
title Bordetella parapertussis Circumvents Neutrophil Extracellular Bactericidal Mechanisms
title_short Bordetella parapertussis Circumvents Neutrophil Extracellular Bactericidal Mechanisms
title_full Bordetella parapertussis Circumvents Neutrophil Extracellular Bactericidal Mechanisms
title_fullStr Bordetella parapertussis Circumvents Neutrophil Extracellular Bactericidal Mechanisms
title_full_unstemmed Bordetella parapertussis Circumvents Neutrophil Extracellular Bactericidal Mechanisms
title_sort bordetella parapertussis circumvents neutrophil extracellular bactericidal mechanisms
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/87579
work_keys_str_mv AT gorgojojuanpablo bordetellaparapertussiscircumventsneutrophilextracellularbactericidalmechanisms
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bdutipo_str Repositorios
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