Biochemical and structural characterization of a novel arginine kinase from the spider <i>Polybetes pythagoricus</i>

Energy buffering systems are key for homeostasis during variations in energy supply. Spiders are the most important predators for insects and therefore key in terrestrial ecosystems. From biomedical interest, spiders are important for their venoms and as a source of potent allergens, such as arginin...

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Detalles Bibliográficos
Autores principales:	Laino, Aldana, Lopez Zavala, Alonso A., Garcia Orozco, Karina D., Carrasco Miranda, Jesus S., Santana, Marianela, Stojanoff, Vivian, Sotelo Mundo, Rogelio R., García, Carlos Fernando
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2017
Materias:	Ciencias Naturales Ciencias Exactas Allergen Arachnida Arginine kinase Arthropoda cDNA cloning Crystal structure Open conformation Phosphagen Polybetes pytagoricus Spider
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/87537
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-87537
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Naturales Ciencias Exactas Allergen Arachnida Arginine kinase Arthropoda cDNA cloning Crystal structure Open conformation Phosphagen Polybetes pytagoricus Spider
spellingShingle	Ciencias Naturales Ciencias Exactas Allergen Arachnida Arginine kinase Arthropoda cDNA cloning Crystal structure Open conformation Phosphagen Polybetes pytagoricus Spider Laino, Aldana Lopez Zavala, Alonso A. Garcia Orozco, Karina D. Carrasco Miranda, Jesus S. Santana, Marianela Stojanoff, Vivian Sotelo Mundo, Rogelio R. García, Carlos Fernando Biochemical and structural characterization of a novel arginine kinase from the spider <i>Polybetes pythagoricus</i>
topic_facet	Ciencias Naturales Ciencias Exactas Allergen Arachnida Arginine kinase Arthropoda cDNA cloning Crystal structure Open conformation Phosphagen Polybetes pytagoricus Spider
description	Energy buffering systems are key for homeostasis during variations in energy supply. Spiders are the most important predators for insects and therefore key in terrestrial ecosystems. From biomedical interest, spiders are important for their venoms and as a source of potent allergens, such as arginine kinase (AK, EC 2.7.3.3). AK is an enzyme crucial for energy metabolism, keeping the pool of phosphagens in invertebrates, and also an allergen for humans. In this work, we studied AK from the Argentininan spider <i>Polybetes pythagoricus</i> (PpAK), from its complementary DNA to the crystal structure. The PpAK cDNA from muscle was cloned, and it is comprised of 1068 nucleotides that encode a 384-amino acids protein, similar to other invertebrate AKs. The apparent Michaelis-Menten kinetic constant (K<sub>m</sub>) was 1.7 mM with a k<sub>cat</sub> of 75 s<sup>-1</sup>. Two crystal structures are presented, the apoPvAK and PpAK bound to arginine, both in the open conformation with the active site lid (residues 310-320) completely disordered. The guanidino group binding site in the apo structure appears to be organized to accept the arginine substrate. Finally, these results contribute to knowledge of mechanistic details of the function of arginine kinase.
format	Articulo Articulo
author	Laino, Aldana Lopez Zavala, Alonso A. Garcia Orozco, Karina D. Carrasco Miranda, Jesus S. Santana, Marianela Stojanoff, Vivian Sotelo Mundo, Rogelio R. García, Carlos Fernando
author_facet	Laino, Aldana Lopez Zavala, Alonso A. Garcia Orozco, Karina D. Carrasco Miranda, Jesus S. Santana, Marianela Stojanoff, Vivian Sotelo Mundo, Rogelio R. García, Carlos Fernando
author_sort	Laino, Aldana
title	Biochemical and structural characterization of a novel arginine kinase from the spider <i>Polybetes pythagoricus</i>
title_short	Biochemical and structural characterization of a novel arginine kinase from the spider <i>Polybetes pythagoricus</i>
title_full	Biochemical and structural characterization of a novel arginine kinase from the spider <i>Polybetes pythagoricus</i>
title_fullStr	Biochemical and structural characterization of a novel arginine kinase from the spider <i>Polybetes pythagoricus</i>
title_full_unstemmed	Biochemical and structural characterization of a novel arginine kinase from the spider <i>Polybetes pythagoricus</i>
title_sort	biochemical and structural characterization of a novel arginine kinase from the spider <i>polybetes pythagoricus</i>
publishDate	2017
url	http://sedici.unlp.edu.ar/handle/10915/87537
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bdutipo_str	Repositorios
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Biochemical and structural characterization of a novel arginine kinase from the spider <i>Polybetes pythagoricus</i>

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