Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs

Malaysia has a great number of hot springs, especially along the flank of the Banjaran Titiwangsa mountain range. Biological studies of the Malaysian hot springs are rare because of the lack of comprehensive information on their microbial communities. In this study, we report a cultivation-independe...

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Autores principales:	Chan, Chia S., Chan, Kok-Gan, Ee, Robson, Hong, Kar-Wai, Urbieta, María Sofía, Donati, Edgardo Rubén, Shamsir, Mohd S., Goh, Kian M.
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2017
Materias:	Ciencias Exactas 16S rRNA amplicon sequencing Hot spring metagenome Microbial community Microbial symbiosis Microbiome Saline pool Thermophile diversity
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/87466
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-87466
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Exactas 16S rRNA amplicon sequencing Hot spring metagenome Microbial community Microbial symbiosis Microbiome Saline pool Thermophile diversity
spellingShingle	Ciencias Exactas 16S rRNA amplicon sequencing Hot spring metagenome Microbial community Microbial symbiosis Microbiome Saline pool Thermophile diversity Chan, Chia S. Chan, Kok-Gan Ee, Robson Hong, Kar-Wai Urbieta, María Sofía Donati, Edgardo Rubén Shamsir, Mohd S. Goh, Kian M. Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs
topic_facet	Ciencias Exactas 16S rRNA amplicon sequencing Hot spring metagenome Microbial community Microbial symbiosis Microbiome Saline pool Thermophile diversity
description	Malaysia has a great number of hot springs, especially along the flank of the Banjaran Titiwangsa mountain range. Biological studies of the Malaysian hot springs are rare because of the lack of comprehensive information on their microbial communities. In this study, we report a cultivation-independent census to describe microbial communities in six hot springs. The Ulu Slim (US), Sungai Klah (SK), Dusun Tua (DT), Sungai Serai (SS), Semenyih (SE), and Ayer Hangat (AH) hot springs exhibit circumneutral pH with temperatures ranging from 43°C to 90°C. Genomic DNA was extracted from environmental samples and the V3-V4 hypervariable regions of 16S rRNA genes were amplified, sequenced, and analyzed. High-throughput sequencing analysis showed that microbial richness was high in all samples as indicated by the detection of 6,334-26,244 operational taxonomy units. In total, 59, 61, 72, 73, 65, and 52 bacterial phyla were identified in the US, SK, DT, SS, SE, and AH hot springs, respectively. Generally, Firmicutes and Proteobacteria dominated the bacterial communities in all hot springs. Archaeal communities mainly consisted of Crenarchaeota, Euryarchaeota, and Parvarchaeota. In beta diversity analysis, the hot spring microbial memberships were clustered primarily on the basis of temperature and salinity. Canonical correlation analysis to assess the relationship between the microbial communities and physicochemical variables revealed that diversity patterns were best explained by a combination of physicochemical variables, rather than by individual abiotic variables such as temperature and salinity.
format	Articulo Articulo
author	Chan, Chia S. Chan, Kok-Gan Ee, Robson Hong, Kar-Wai Urbieta, María Sofía Donati, Edgardo Rubén Shamsir, Mohd S. Goh, Kian M.
author_facet	Chan, Chia S. Chan, Kok-Gan Ee, Robson Hong, Kar-Wai Urbieta, María Sofía Donati, Edgardo Rubén Shamsir, Mohd S. Goh, Kian M.
author_sort	Chan, Chia S.
title	Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs
title_short	Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs
title_full	Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs
title_fullStr	Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs
title_full_unstemmed	Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs
title_sort	effects of physiochemical factors on prokaryotic biodiversity in malaysian circumneutral hot springs
publishDate	2017
url	http://sedici.unlp.edu.ar/handle/10915/87466
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Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs

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