Transcriptional control of the lateral-flagellar genes of Bradyrhizobium diazoefficiens

Bradyrhizobium diazoefficiens, a soybean N2-fixing symbiont, possesses a dual flagellar system comprising a constitutive subpolar flagellum and inducible lateral flagella. Here, we analyzed the genomic organization and biosynthetic regulation of the lateral-flagellar genes. We found that these genes...

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Detalles Bibliográficos
Autores principales: Mongiardini, Elías Javier, Quelas, Juan Ignacio, Dardis, Carolina, Althabegoiti, María Julia, Lodeiro, Aníbal Roberto
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Biología
Bradyrhizobium
Expression
Flagella
FlbT
LafR
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/87303
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-87303
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Bradyrhizobium
Expression
Flagella
FlbT
FlbT
LafR
LafR
spellingShingle Biología
Bradyrhizobium
Expression
Flagella
FlbT
FlbT
LafR
LafR
Mongiardini, Elías Javier
Quelas, Juan Ignacio
Dardis, Carolina
Althabegoiti, María Julia
Lodeiro, Aníbal Roberto
Transcriptional control of the lateral-flagellar genes of Bradyrhizobium diazoefficiens
topic_facet Biología
Bradyrhizobium
Expression
Flagella
FlbT
FlbT
LafR
LafR
description Bradyrhizobium diazoefficiens, a soybean N2-fixing symbiont, possesses a dual flagellar system comprising a constitutive subpolar flagellum and inducible lateral flagella. Here, we analyzed the genomic organization and biosynthetic regulation of the lateral-flagellar genes. We found that these genes are located in a single genomic cluster, organized in two monocistronic transcriptional units and three operons, one possibly containing an internal transcription start site. Among the monocistronic units is blr6846, homologous to the class IB master regulators of flagellum synthesis in Brucella melitensis and Ensifer meliloti and required for the expression of all the lateral-flagellar genes except lafA2, whose locus encodes a single lateral flagellin. We therefore named blr6846 lafR (lateral-flagellar regulator). Despite its similarity to two-component response regulators and its possession of a phosphorylatable Asp residue, lafR behaved as an orphan response regulator by not requiring phosphorylation at this site. Among the genes induced by lafR is flbTL, a class III regulator. We observed different requirements for FlbTL in the synthesis of each flagellin subunit. Although the accumulation of lafA1, but not lafA2, transcripts required FlbTL, the production of both flagellin polypeptides required FlbTL. Moreover, the regulation cascade of this lateral-flagellar regulon appeared to be not as strictly ordered as those found in other bacterial species.
format Articulo
Articulo
author Mongiardini, Elías Javier
Quelas, Juan Ignacio
Dardis, Carolina
Althabegoiti, María Julia
Lodeiro, Aníbal Roberto
author_facet Mongiardini, Elías Javier
Quelas, Juan Ignacio
Dardis, Carolina
Althabegoiti, María Julia
Lodeiro, Aníbal Roberto
author_sort Mongiardini, Elías Javier
title Transcriptional control of the lateral-flagellar genes of Bradyrhizobium diazoefficiens
title_short Transcriptional control of the lateral-flagellar genes of Bradyrhizobium diazoefficiens
title_full Transcriptional control of the lateral-flagellar genes of Bradyrhizobium diazoefficiens
title_fullStr Transcriptional control of the lateral-flagellar genes of Bradyrhizobium diazoefficiens
title_full_unstemmed Transcriptional control of the lateral-flagellar genes of Bradyrhizobium diazoefficiens
title_sort transcriptional control of the lateral-flagellar genes of bradyrhizobium diazoefficiens
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/87303
work_keys_str_mv AT mongiardinieliasjavier transcriptionalcontrolofthelateralflagellargenesofbradyrhizobiumdiazoefficiens
AT quelasjuanignacio transcriptionalcontrolofthelateralflagellargenesofbradyrhizobiumdiazoefficiens
AT dardiscarolina transcriptionalcontrolofthelateralflagellargenesofbradyrhizobiumdiazoefficiens
AT althabegoitimariajulia transcriptionalcontrolofthelateralflagellargenesofbradyrhizobiumdiazoefficiens
AT lodeiroanibalroberto transcriptionalcontrolofthelateralflagellargenesofbradyrhizobiumdiazoefficiens
bdutipo_str Repositorios
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