Bioinformatic and mutational analysis of ophiovirus movement proteins, belonging to the 30K superfamily

Ophioviridae is a family of segmented, negative-sense, single-stranded RNA plant viruses. We showed that their cell-to-cell movement protein (MP) is an isolated member of the 30K MP superfamily with a unique structural organization. All 30K MPs share a core domain that contains a nearly-invariant si...

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Detalles Bibliográficos
Autores principales:	Borniego, María Belén, Karlin, David, Peña, Eduardo José, Robles Luna, Gabriel, García, María Laura
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2016
Materias:	Biología 30K superfamily Citrus psorosis virus Mirafiori lettuce big-vein virus Movement protein Negative-strand RNA virus Ophiovirus TMV vector
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/85821
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

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record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Biología 30K superfamily Citrus psorosis virus Mirafiori lettuce big-vein virus Movement protein Negative-strand RNA virus Ophiovirus TMV vector
spellingShingle	Biología 30K superfamily Citrus psorosis virus Mirafiori lettuce big-vein virus Movement protein Negative-strand RNA virus Ophiovirus TMV vector Borniego, María Belén Karlin, David Peña, Eduardo José Robles Luna, Gabriel García, María Laura Bioinformatic and mutational analysis of ophiovirus movement proteins, belonging to the 30K superfamily
topic_facet	Biología 30K superfamily Citrus psorosis virus Mirafiori lettuce big-vein virus Movement protein Negative-strand RNA virus Ophiovirus TMV vector
description	Ophioviridae is a family of segmented, negative-sense, single-stranded RNA plant viruses. We showed that their cell-to-cell movement protein (MP) is an isolated member of the 30K MP superfamily with a unique structural organization. All 30K MPs share a core domain that contains a nearly-invariant signature aspartate. We examined its role in the MP of Citrus psorosis virus (CPsV) and Mirafiori lettuce big-vein virus (MiLBVV). Alanine substitution of this aspartate prevented plasmodesmata accumulation of MPMiLBVV, while MPCPsV was not affected. The capacity of ophiovirus MPs to increase the plasmodesmata size exclusion limit and non-cell autonomous protein feature was abolished in both mutants. To investigate the role of the signature aspartate in cell-to-cell movement, we constructed a new movement-deficient Tobacco mosaic virus vector used for trans-complementation assays. We showed that both ophiovirus MP mutants lack the cell-to-cell movement capacity, confirming that this signature aspartate is essential for viral cell-to-cell movement.
format	Articulo Articulo
author	Borniego, María Belén Karlin, David Peña, Eduardo José Robles Luna, Gabriel García, María Laura
author_facet	Borniego, María Belén Karlin, David Peña, Eduardo José Robles Luna, Gabriel García, María Laura
author_sort	Borniego, María Belén
title	Bioinformatic and mutational analysis of ophiovirus movement proteins, belonging to the 30K superfamily
title_short	Bioinformatic and mutational analysis of ophiovirus movement proteins, belonging to the 30K superfamily
title_full	Bioinformatic and mutational analysis of ophiovirus movement proteins, belonging to the 30K superfamily
title_fullStr	Bioinformatic and mutational analysis of ophiovirus movement proteins, belonging to the 30K superfamily
title_full_unstemmed	Bioinformatic and mutational analysis of ophiovirus movement proteins, belonging to the 30K superfamily
title_sort	bioinformatic and mutational analysis of ophiovirus movement proteins, belonging to the 30k superfamily
publishDate	2016
url	http://sedici.unlp.edu.ar/handle/10915/85821
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Bioinformatic and mutational analysis of ophiovirus movement proteins, belonging to the 30K superfamily

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