Bordetella pertussis isolates from argentinean whooping cough patients display enhanced biofilm formation capacity compared to Tohama I reference strain

Pertussis is a highly contagious disease mainly caused by <i>Bordetella pertussis</i>. Despite the massive use of vaccines, since the 1950s the disease has become re-emergent in 2000 with a shift in incidence from infants to adolescents and adults. Clearly, the efficacy of current cellul...

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Autores principales: Arnal, Laura, Grunert, Tom, Cattelan, Natalia, Gouw, Dann de, Villalba, María Inés, Serra, Diego Omar, Mooi, Frits R., Ehling Schulz, Monika, Yantorno, Osvaldo Miguel
Formato: Articulo
Lenguaje:Inglés
Publicado: 2015
Materias:
Ciencias Exactas
Biofilm
Bordetella pertussis
Clinical isolates
Proteomic
Real time PCR
Whooping cough
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/85736
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-85736
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Biofilm
Bordetella pertussis
Clinical isolates
Proteomic
Real time PCR
Whooping cough
spellingShingle Ciencias Exactas
Biofilm
Bordetella pertussis
Clinical isolates
Proteomic
Real time PCR
Whooping cough
Arnal, Laura
Grunert, Tom
Cattelan, Natalia
Gouw, Dann de
Villalba, María Inés
Serra, Diego Omar
Mooi, Frits R.
Ehling Schulz, Monika
Yantorno, Osvaldo Miguel
Bordetella pertussis isolates from argentinean whooping cough patients display enhanced biofilm formation capacity compared to Tohama I reference strain
topic_facet Ciencias Exactas
Biofilm
Bordetella pertussis
Clinical isolates
Proteomic
Real time PCR
Whooping cough
description Pertussis is a highly contagious disease mainly caused by <i>Bordetella pertussis</i>. Despite the massive use of vaccines, since the 1950s the disease has become re-emergent in 2000 with a shift in incidence from infants to adolescents and adults. Clearly, the efficacy of current cellular or acellular vaccines, formulated from bacteria grown in stirred bioreactors is limited, presenting a challenge for future vaccine development. For gaining insights into the role of <i>B. pertussis</i> biofilm development for host colonization and persistence within the host, we examined the biofilm forming capacity of eight argentinean clinical isolates recovered from 2001 to 2007. All clinical isolates showed an enhanced potential for biofilm formation compared to the reference strain Tohama I. We further selected the clinical isolate <i>B. pertussis</i> 2723, exhibiting the highest biofilm biomass production, for quantitative proteomic profiling by means of two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) coupled with mass spectrometry, which was accompanied by targeted transcriptional analysis. Results revealed an elevated expression of several virulence factors, including adhesins involved in biofilm development. In addition, we observed a higher expression of energy metabolism enzymes in the clinical isolate compared to the Tohama I strain. Furthermore, all clinical isolates carried a polymorphism in the <i>bvgS</i> gene. This mutation was associated to an increased sensitivity to modulation and a faster rate of adhesion to abiotic surfaces. Thus, the phenotypic biofilm characteristics shown by the clinical isolates might represent an important, hitherto underestimated, adaptive strategy for host colonization and long time persistence within the host.
format Articulo
Articulo
author Arnal, Laura
Grunert, Tom
Cattelan, Natalia
Gouw, Dann de
Villalba, María Inés
Serra, Diego Omar
Mooi, Frits R.
Ehling Schulz, Monika
Yantorno, Osvaldo Miguel
author_facet Arnal, Laura
Grunert, Tom
Cattelan, Natalia
Gouw, Dann de
Villalba, María Inés
Serra, Diego Omar
Mooi, Frits R.
Ehling Schulz, Monika
Yantorno, Osvaldo Miguel
author_sort Arnal, Laura
title Bordetella pertussis isolates from argentinean whooping cough patients display enhanced biofilm formation capacity compared to Tohama I reference strain
title_short Bordetella pertussis isolates from argentinean whooping cough patients display enhanced biofilm formation capacity compared to Tohama I reference strain
title_full Bordetella pertussis isolates from argentinean whooping cough patients display enhanced biofilm formation capacity compared to Tohama I reference strain
title_fullStr Bordetella pertussis isolates from argentinean whooping cough patients display enhanced biofilm formation capacity compared to Tohama I reference strain
title_full_unstemmed Bordetella pertussis isolates from argentinean whooping cough patients display enhanced biofilm formation capacity compared to Tohama I reference strain
title_sort bordetella pertussis isolates from argentinean whooping cough patients display enhanced biofilm formation capacity compared to tohama i reference strain
publishDate 2015
url http://sedici.unlp.edu.ar/handle/10915/85736
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