Inoculation of Triatoma Virus (Dicistroviridae: Cripavirus) elicits a non-infective immune response in mice

Background: Dicistroviridae is a new family of small, non-enveloped, +ssRNA viruses pathogenic to both beneficial arthropods and insect pests. Little is known about the dicistrovirus replication mechanism or gene function, and any knowledge on these subjects comes mainly from comparisons with mammal...

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Detalles Bibliográficos
Autores principales: Querido, J. F. B., Agirre, J., Martí, Gerardo Aníbal, Guérin, Diego M. A., Sousa Silva, M.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2013
Materias:
Ciencias Naturales
Chagas disease
Dicistroviridae
Mice immune response
Triatoma virus
triatomines
Trypanosoma cruzi
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/85682
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-85682
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Naturales
Chagas disease
Dicistroviridae
Mice immune response
Triatoma virus
triatomines
Trypanosoma cruzi
spellingShingle Ciencias Naturales
Chagas disease
Dicistroviridae
Mice immune response
Triatoma virus
triatomines
Trypanosoma cruzi
Querido, J. F. B.
Agirre, J.
Martí, Gerardo Aníbal
Guérin, Diego M. A.
Sousa Silva, M.
Inoculation of Triatoma Virus (Dicistroviridae: Cripavirus) elicits a non-infective immune response in mice
topic_facet Ciencias Naturales
Chagas disease
Dicistroviridae
Mice immune response
Triatoma virus
triatomines
Trypanosoma cruzi
description Background: Dicistroviridae is a new family of small, non-enveloped, +ssRNA viruses pathogenic to both beneficial arthropods and insect pests. Little is known about the dicistrovirus replication mechanism or gene function, and any knowledge on these subjects comes mainly from comparisons with mammalian viruses from the Picornaviridae family. Due to its peculiar genome organization and characteristics of the per os viral transmission route, dicistroviruses make good candidates for use as biopesticides. Triatoma virus (TrV) is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of the human trypanosomiasis disease called Chagas disease. TrV was postulated as a potential control agent against Chagas' vectors. Although there is no evidence that TrV nor other dicistroviruses replicate in species outside the Insecta class, the innocuousness of these viruses in humans and animals needs to be ascertained. Methods. In this study, RT-PCR and ELISA were used to detect the infectivity of this virus in Mus musculus BALB/c mice. Results: In this study we have observed that there is no significant difference in the ratio IgG2a/IgG1 in sera from animals inoculated with TrV when compared with non-inoculated animals or mice inoculated only with non-infective TrV protein capsids. Conclusions: We conclude that, under our experimental conditions, TrV is unable to replicate in mice. This study constitutes the first test to evaluate the infectivity of a dicistrovirus in a vertebrate animal model.
format Articulo
Articulo
author Querido, J. F. B.
Agirre, J.
Martí, Gerardo Aníbal
Guérin, Diego M. A.
Sousa Silva, M.
author_facet Querido, J. F. B.
Agirre, J.
Martí, Gerardo Aníbal
Guérin, Diego M. A.
Sousa Silva, M.
author_sort Querido, J. F. B.
title Inoculation of Triatoma Virus (Dicistroviridae: Cripavirus) elicits a non-infective immune response in mice
title_short Inoculation of Triatoma Virus (Dicistroviridae: Cripavirus) elicits a non-infective immune response in mice
title_full Inoculation of Triatoma Virus (Dicistroviridae: Cripavirus) elicits a non-infective immune response in mice
title_fullStr Inoculation of Triatoma Virus (Dicistroviridae: Cripavirus) elicits a non-infective immune response in mice
title_full_unstemmed Inoculation of Triatoma Virus (Dicistroviridae: Cripavirus) elicits a non-infective immune response in mice
title_sort inoculation of triatoma virus (dicistroviridae: cripavirus) elicits a non-infective immune response in mice
publishDate 2013
url http://sedici.unlp.edu.ar/handle/10915/85682
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