Glycerol-3-phosphate acyltranferase-2 behaves as a cancer testis gene and promotes growth and tumorigenicity of the breast cancer MDA-MB-231 cell line

The de novo synthesis of glycerolipids in mammalian cells begins with the acylation of glycerol-3-phosphate, catalyzed by glycerol-3-phosphate acyltransferase (GPAT). GPAT2 is a mitochondrial isoform primarily expressed in testis under physiological conditions. Because it is aberrantly expressed in...

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Detalles Bibliográficos
Autores principales: Pellón Maisón, Magalí, Montanaro, Mauro Aldo, Lacunza, Ezequiel, García Fabiani, María Belén, Soler Gerino, Mercedes C., Cattaneo, Elizabeth Renee, Quiroga, Ivana Yoseli, Abba, Martín Carlos, Coleman, Rosalind A., González Baró, María del Rosario
Formato: Articulo
Lenguaje:Inglés
Publicado: 2014
Materias:
Ciencias Médicas
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/85619
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-85619
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
spellingShingle Ciencias Médicas
Pellón Maisón, Magalí
Montanaro, Mauro Aldo
Lacunza, Ezequiel
García Fabiani, María Belén
Soler Gerino, Mercedes C.
Cattaneo, Elizabeth Renee
Quiroga, Ivana Yoseli
Abba, Martín Carlos
Coleman, Rosalind A.
González Baró, María del Rosario
Glycerol-3-phosphate acyltranferase-2 behaves as a cancer testis gene and promotes growth and tumorigenicity of the breast cancer MDA-MB-231 cell line
topic_facet Ciencias Médicas
description The de novo synthesis of glycerolipids in mammalian cells begins with the acylation of glycerol-3-phosphate, catalyzed by glycerol-3-phosphate acyltransferase (GPAT). GPAT2 is a mitochondrial isoform primarily expressed in testis under physiological conditions. Because it is aberrantly expressed in multiple myeloma, it has been proposed as a novel cancer testis gene. Using a bioinformatics approach, we found that GPAT2 is highly expressed in melanoma, lung, prostate and breast cancer, and we validated GPAT2 expression at the protein level in breast cancer by immunohistochemistry. In this case GPAT2 expression correlated with a higher histological grade. 5-Aza-2′ deoxycytidine treatment of human cells lines induced GPAT2 expression suggesting epigenetic regulation of gene expression. In order to evaluate the contribution of GPAT2 to the tumor phenotype, we silenced its expression in MDA-MB-231 cells. GPAT2 knockdown diminished cell proliferation, anchorage independent growth, migration and tumorigenicity, and increased staurosporine-induced apoptosis. In contrast, GPAT2 over-expression increased cell proliferation rate and resistance to staurosporine-induced apoptosis. To understand the functional role of GPAT2, we performed a co-expression analysis in mouse and human testis and found a significant association with semantic terms involved in cell cycle, DNA integrity maintenance, piRNA biogenesis and epigenetic regulation. Overall, these results indicate the GPAT2 would be directly associated with the control of cell proliferation. In conclusion, we confirm GPAT2 as a cancer testis gene and that its expression contributes to the tumor phenotype of MDA-MB-231 cells.
format Articulo
Articulo
author Pellón Maisón, Magalí
Montanaro, Mauro Aldo
Lacunza, Ezequiel
García Fabiani, María Belén
Soler Gerino, Mercedes C.
Cattaneo, Elizabeth Renee
Quiroga, Ivana Yoseli
Abba, Martín Carlos
Coleman, Rosalind A.
González Baró, María del Rosario
author_facet Pellón Maisón, Magalí
Montanaro, Mauro Aldo
Lacunza, Ezequiel
García Fabiani, María Belén
Soler Gerino, Mercedes C.
Cattaneo, Elizabeth Renee
Quiroga, Ivana Yoseli
Abba, Martín Carlos
Coleman, Rosalind A.
González Baró, María del Rosario
author_sort Pellón Maisón, Magalí
title Glycerol-3-phosphate acyltranferase-2 behaves as a cancer testis gene and promotes growth and tumorigenicity of the breast cancer MDA-MB-231 cell line
title_short Glycerol-3-phosphate acyltranferase-2 behaves as a cancer testis gene and promotes growth and tumorigenicity of the breast cancer MDA-MB-231 cell line
title_full Glycerol-3-phosphate acyltranferase-2 behaves as a cancer testis gene and promotes growth and tumorigenicity of the breast cancer MDA-MB-231 cell line
title_fullStr Glycerol-3-phosphate acyltranferase-2 behaves as a cancer testis gene and promotes growth and tumorigenicity of the breast cancer MDA-MB-231 cell line
title_full_unstemmed Glycerol-3-phosphate acyltranferase-2 behaves as a cancer testis gene and promotes growth and tumorigenicity of the breast cancer MDA-MB-231 cell line
title_sort glycerol-3-phosphate acyltranferase-2 behaves as a cancer testis gene and promotes growth and tumorigenicity of the breast cancer mda-mb-231 cell line
publishDate 2014
url http://sedici.unlp.edu.ar/handle/10915/85619
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