Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA 2 and interacts with the coat protein

Citrus psorosis virus (CPsV) and Mirafiori lettuce big-vein virus (MiLBVV), members of the Ophioviridae family, have segmented negative-sense single-stranded RNA genomes. To date no reports have described how ophioviruses spread within host pl...

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Autores principales:	Robles Luna, Gabriel, Peña, Eduardo José, Borniego, María Belén, Heinlein, Manfred, García, María Laura
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2013
Materias:	Ciencias Exactas Citrus psorosis virus Mirafiori lettuce big-vein virus Movement protein Negative-strand RNA virus Ophioviridae Protein-protein Interaction
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/85543
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-85543
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Exactas Citrus psorosis virus Mirafiori lettuce big-vein virus Movement protein Negative-strand RNA virus Ophioviridae Protein-protein Interaction
spellingShingle	Ciencias Exactas Citrus psorosis virus Mirafiori lettuce big-vein virus Movement protein Negative-strand RNA virus Ophioviridae Protein-protein Interaction Robles Luna, Gabriel Peña, Eduardo José Borniego, María Belén Heinlein, Manfred García, María Laura Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA 2 and interacts with the coat protein
topic_facet	Ciencias Exactas Citrus psorosis virus Mirafiori lettuce big-vein virus Movement protein Negative-strand RNA virus Ophioviridae Protein-protein Interaction
description	<i>Citrus psorosis virus</i> (CPsV) and <i>Mirafiori lettuce big-vein virus</i> (MiLBVV), members of the <i>Ophioviridae</i> family, have segmented negative-sense single-stranded RNA genomes. To date no reports have described how ophioviruses spread within host plants and/or the proteins involved in this process. Here we show that the 54K protein of CPsV is encoded by RNA 2 and describe its subcellular distribution. Upon transient expression in <i>Nicotiana benthamiana</i> epidermal cells the 54K protein, and also its 54K counterpart protein of MiLBVV, localize to plasmodesmata and enhance GFP cell-to-cell diffusion between cells. Both proteins, but not the coat proteins (CP) of the respective viruses, functionally <i>trans</i>-complement cell-to-cell movement-defective <i>Potato virus</i> X (PVX) and <i>Tobacco mosaic virus</i> (TMV) mutants. The 54K and 54K proteins interact with the virus-specific CP in the cytoplasm, suggesting a potential role of CP in ophiovirus movement. This is the first study characterizing the movement proteins (MP) of ophioviruses.
format	Articulo Articulo
author	Robles Luna, Gabriel Peña, Eduardo José Borniego, María Belén Heinlein, Manfred García, María Laura
author_facet	Robles Luna, Gabriel Peña, Eduardo José Borniego, María Belén Heinlein, Manfred García, María Laura
author_sort	Robles Luna, Gabriel
title	Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA 2 and interacts with the coat protein
title_short	Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA 2 and interacts with the coat protein
title_full	Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA 2 and interacts with the coat protein
title_fullStr	Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA 2 and interacts with the coat protein
title_full_unstemmed	Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA 2 and interacts with the coat protein
title_sort	ophioviruses cpsv and milbvv movement protein is encoded in rna 2 and interacts with the coat protein
publishDate	2013
url	http://sedici.unlp.edu.ar/handle/10915/85543
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bdutipo_str	Repositorios
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Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA 2 and interacts with the coat protein

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