Rhizobial plasmid pLPU83a is able to switch between different transfer machineries depending on its genomic background

Plasmids have played a major role in bacterial evolution, mainly by their capacity to perform horizontal gene transfer (HGT). Their conjugative transfer (CT) properties are usually described in terms of the plasmid itself. In this work, we analyzed structural and functional aspects of the CT of pLPU...

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Autores principales:	Torres Tejerizo, Gonzalo Arturo, Pistorio, Mariano, Althabegoiti, María Julia, Cervantes, Laura, Wibberg, Daniel, Schlüter, Andreas, Pühler, Alfred, Lagares, Antonio, Romero, David, Brom, Susana
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2014
Materias:	Ciencias Exactas Conjugative transfer regulation LuxR regulators Plasmid Rhizobia
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/85262
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-85262
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Exactas Conjugative transfer regulation LuxR regulators Plasmid Rhizobia
spellingShingle	Ciencias Exactas Conjugative transfer regulation LuxR regulators Plasmid Rhizobia Torres Tejerizo, Gonzalo Arturo Pistorio, Mariano Althabegoiti, María Julia Cervantes, Laura Wibberg, Daniel Schlüter, Andreas Pühler, Alfred Lagares, Antonio Romero, David Brom, Susana Rhizobial plasmid pLPU83a is able to switch between different transfer machineries depending on its genomic background
topic_facet	Ciencias Exactas Conjugative transfer regulation LuxR regulators Plasmid Rhizobia
description	Plasmids have played a major role in bacterial evolution, mainly by their capacity to perform horizontal gene transfer (HGT). Their conjugative transfer (CT) properties are usually described in terms of the plasmid itself. In this work, we analyzed structural and functional aspects of the CT of pLPU83a, an accessory replicon from <i>Rhizobium</i> sp. LPU83, able to transfer from its parental strain, from <i>Ensifer meliloti</i>, or from <i>Rhizobium etli</i>. pLPU83a contains a complete set of transfer genes, featuring a particular organization, shared with only two other rhizobial plasmids. These plasmids contain a TraR quorum-sensing (QS) transcriptional regulator, but lack an acyl-homoserine lactone (AHL) synthase gene. We also determined that the ability of pLPU83a to transfer from <i>R. etli</i> CFN42 genomic background was mainly achieved through mobilization, employing the machinery of the endogenous plasmid pRetCFN42a, falling under control of the QS regulators from pRetCFN42a. In contrast, from its native or from the <i>E. meliloti</i> background, pLPU83a utilized its own machinery for conjugation, requiring the plasmid-encoded <i>traR</i>. Activation of TraR seemed to be AHL independent. The results obtained indicate that the CT phenotype of a plasmid is dictated not only by the genes it carries, but by their interaction with its genomic context.
format	Articulo Articulo
author	Torres Tejerizo, Gonzalo Arturo Pistorio, Mariano Althabegoiti, María Julia Cervantes, Laura Wibberg, Daniel Schlüter, Andreas Pühler, Alfred Lagares, Antonio Romero, David Brom, Susana
author_facet	Torres Tejerizo, Gonzalo Arturo Pistorio, Mariano Althabegoiti, María Julia Cervantes, Laura Wibberg, Daniel Schlüter, Andreas Pühler, Alfred Lagares, Antonio Romero, David Brom, Susana
author_sort	Torres Tejerizo, Gonzalo Arturo
title	Rhizobial plasmid pLPU83a is able to switch between different transfer machineries depending on its genomic background
title_short	Rhizobial plasmid pLPU83a is able to switch between different transfer machineries depending on its genomic background
title_full	Rhizobial plasmid pLPU83a is able to switch between different transfer machineries depending on its genomic background
title_fullStr	Rhizobial plasmid pLPU83a is able to switch between different transfer machineries depending on its genomic background
title_full_unstemmed	Rhizobial plasmid pLPU83a is able to switch between different transfer machineries depending on its genomic background
title_sort	rhizobial plasmid plpu83a is able to switch between different transfer machineries depending on its genomic background
publishDate	2014
url	http://sedici.unlp.edu.ar/handle/10915/85262
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Rhizobial plasmid pLPU83a is able to switch between different transfer machineries depending on its genomic background

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