The stimulated innate resistance event in Bordetella pertussis infection is dependent on reactive oxygen species production

The exacerbated induction of innate immune responses in airways can abrogate diverse lung infections by a phenomenon known as stimulated innate resistance (StIR). We recently demonstrated that the enhancement of innate response activation can efficiently impair Bordetella pertussis colonization in a...

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Detalles Bibliográficos
Autores principales: Zurita, Alfredo Eduardo, Moreno, Griselda, Errea, Agustina Juliana, Ormazabal, Maximiliano, Rumbo, Martín, Hozbor, Daniela Flavia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2013
Materias:
Biología
Bordetella pertussis
lipopolysaccharide
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/85255
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-85255
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Bordetella pertussis
lipopolysaccharide
spellingShingle Biología
Bordetella pertussis
lipopolysaccharide
Zurita, Alfredo Eduardo
Moreno, Griselda
Errea, Agustina Juliana
Ormazabal, Maximiliano
Rumbo, Martín
Hozbor, Daniela Flavia
The stimulated innate resistance event in Bordetella pertussis infection is dependent on reactive oxygen species production
topic_facet Biología
Bordetella pertussis
lipopolysaccharide
description The exacerbated induction of innate immune responses in airways can abrogate diverse lung infections by a phenomenon known as stimulated innate resistance (StIR). We recently demonstrated that the enhancement of innate response activation can efficiently impair Bordetella pertussis colonization in a Toll-like receptor 4 (TLR4)-dependent manner. The aim of this work was to further characterize the effect of lipopolysaccharide (LPS) on StIR and to identify the mechanisms that mediate this process. Our results showed that bacterial infection was completely abrogated in treated mice when the LPS of B. pertussis (1 μg) was added before (48h or 24h), after (24h), or simultaneously with the B. pertussis challenge (107 CFU). Moreover, we detected that LPS completely cleared bacterial infection as soon as 2 h posttreatment. This timing suggests that the observed StIR phenomenon should be mediated by fast-acting antimicrobial mechanisms. Although neutrophil recruitment was already evident at this time point, depletion assays using an anti-GR1 antibody showed that B. pertussis clearance was achieved even in the absence of neutrophils. To evaluate the possible role of free radicals in StIR, we performed animal assays using the antioxidant N-acetyl cysteine (NAC), which is known to inactivate oxidant species. NAC administration blocked the B. pertussis clearance induced by LPS. Nitrite concentrations were also increased in the LPS-treated mice; however, the inhibition of nitric oxide synthetases did not suppress the LPS-induced bacterial clearance. Taken together, our results show that reactive oxygen species (ROS) play an essential role in the TLR4-dependent innate clearance of B. pertussis.
format Articulo
Articulo
author Zurita, Alfredo Eduardo
Moreno, Griselda
Errea, Agustina Juliana
Ormazabal, Maximiliano
Rumbo, Martín
Hozbor, Daniela Flavia
author_facet Zurita, Alfredo Eduardo
Moreno, Griselda
Errea, Agustina Juliana
Ormazabal, Maximiliano
Rumbo, Martín
Hozbor, Daniela Flavia
author_sort Zurita, Alfredo Eduardo
title The stimulated innate resistance event in Bordetella pertussis infection is dependent on reactive oxygen species production
title_short The stimulated innate resistance event in Bordetella pertussis infection is dependent on reactive oxygen species production
title_full The stimulated innate resistance event in Bordetella pertussis infection is dependent on reactive oxygen species production
title_fullStr The stimulated innate resistance event in Bordetella pertussis infection is dependent on reactive oxygen species production
title_full_unstemmed The stimulated innate resistance event in Bordetella pertussis infection is dependent on reactive oxygen species production
title_sort stimulated innate resistance event in bordetella pertussis infection is dependent on reactive oxygen species production
publishDate 2013
url http://sedici.unlp.edu.ar/handle/10915/85255
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