Cross-reactivity between the soybean protein P34 and bovine caseins

<b>Purpose:</b> Soy-based formulas are widely used as dairy substitutes to treat milk allergy patients. However, reactions to soy have been reported in a small proportion of patients with IgE-mediated milk allergies. The aim of this work was to explore whether P34, a mayor soybean allerg...

Descripción completa

Detalles Bibliográficos
Autores principales: Candreva, Ángela María, Smaldini, Paola Lorena, Curciarello, Renata, Cauerhff, A., Fossati, Carlos Alberto, Docena, Guillermo, Petruccelli, Silvana
Formato: Articulo
Lenguaje:Inglés
Publicado: 2014
Materias:
Ciencias Exactas
Química
Cross-reactivity
Food allergy
Milk
P34 protein
Soybeans
Leche de Soja
Alergia e Inmunología
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/85145
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-85145
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Química
Cross-reactivity
Food allergy
Milk
P34 protein
Soybeans
Leche de Soja
Alergia e Inmunología
spellingShingle Ciencias Exactas
Química
Cross-reactivity
Food allergy
Milk
P34 protein
Soybeans
Leche de Soja
Alergia e Inmunología
Candreva, Ángela María
Smaldini, Paola Lorena
Curciarello, Renata
Cauerhff, A.
Fossati, Carlos Alberto
Docena, Guillermo
Petruccelli, Silvana
Cross-reactivity between the soybean protein P34 and bovine caseins
topic_facet Ciencias Exactas
Química
Cross-reactivity
Food allergy
Milk
P34 protein
Soybeans
Leche de Soja
Alergia e Inmunología
description <b>Purpose:</b> Soy-based formulas are widely used as dairy substitutes to treat milk allergy patients. However, reactions to soy have been reported in a small proportion of patients with IgE-mediated milk allergies. The aim of this work was to explore whether P34, a mayor soybean allergen, is involved in this cross-reactivity. <b>Methods:</b> In vitro recognition of P34 was evaluated by immunoblotting, competitive ELISA and basophil activation tests (BAT) using sera from allergic patients. In vivo cross-reactivity was examined using an IgE-mediated milk allergy mouse model. <b>Results:</b> P34 was recognized by IgE antibodies from the sera of milk allergic patients, casein-specific monoclonal antibodies, and sera from milk-allergic mice. Spleen cells from sensitized mice incubated with milk, soy or P34 secreted IL-5 and IL-13, while IFN-γ remained unchanged. In addition, the cutaneous test was positive with cow's milk proteins (CMP) and P34 in the milk allergy mouse model. Moreover, milk-sensitized mice developed immediate symptoms following sublingual exposure to P34. <b>Conclusions:</b> Our results demonstrate that P34 shares epitopes with bovine casein, which is responsible for inducing hypersensitivity symptoms in milk allergic mice. This is the first report of the in vivo cross-allergenicity of P34.
format Articulo
Articulo
author Candreva, Ángela María
Smaldini, Paola Lorena
Curciarello, Renata
Cauerhff, A.
Fossati, Carlos Alberto
Docena, Guillermo
Petruccelli, Silvana
author_facet Candreva, Ángela María
Smaldini, Paola Lorena
Curciarello, Renata
Cauerhff, A.
Fossati, Carlos Alberto
Docena, Guillermo
Petruccelli, Silvana
author_sort Candreva, Ángela María
title Cross-reactivity between the soybean protein P34 and bovine caseins
title_short Cross-reactivity between the soybean protein P34 and bovine caseins
title_full Cross-reactivity between the soybean protein P34 and bovine caseins
title_fullStr Cross-reactivity between the soybean protein P34 and bovine caseins
title_full_unstemmed Cross-reactivity between the soybean protein P34 and bovine caseins
title_sort cross-reactivity between the soybean protein p34 and bovine caseins
publishDate 2014
url http://sedici.unlp.edu.ar/handle/10915/85145
work_keys_str_mv AT candrevaangelamaria crossreactivitybetweenthesoybeanproteinp34andbovinecaseins
AT smaldinipaolalorena crossreactivitybetweenthesoybeanproteinp34andbovinecaseins
AT curciarellorenata crossreactivitybetweenthesoybeanproteinp34andbovinecaseins
AT cauerhffa crossreactivitybetweenthesoybeanproteinp34andbovinecaseins
AT fossaticarlosalberto crossreactivitybetweenthesoybeanproteinp34andbovinecaseins
AT docenaguillermo crossreactivitybetweenthesoybeanproteinp34andbovinecaseins
AT petruccellisilvana crossreactivitybetweenthesoybeanproteinp34andbovinecaseins
bdutipo_str Repositorios
_version_ 1764820489011724290

Ejemplares similares