The vaccine potential of Bordetella pertussis biofilm-derived membrane proteins
Pertussis is an infectious respiratory disease of humans caused by the gram-negative pathogen <i>Bordetella pertussis</i>. The use of acellular pertussis vaccines (aPs) which induce immunity of relative short duration and the emergence of vaccine-adapted strains are thought to have contr...
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Autores principales: | , , , , , , , , |
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Formato: | Articulo |
Lenguaje: | Inglés |
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2014
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Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/85128 |
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I19-R120-10915-85128 |
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institution |
Universidad Nacional de La Plata |
institution_str |
I-19 |
repository_str |
R-120 |
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SEDICI (UNLP) |
language |
Inglés |
topic |
Ciencias Exactas Biofilms BipA Bordetella pertussis Proteomics Vaccination |
spellingShingle |
Ciencias Exactas Biofilms BipA Bordetella pertussis Proteomics Vaccination De Gouw, Daan Serra, Diego Omar Jonge, Marien I. de Hermans, Peter W. M. Wessels, Hans J. C. T. Zomer, Aldert Yantorno, Osvaldo Miguel Diavatopoulos, Dimitri A. Mooi, Frits R. The vaccine potential of Bordetella pertussis biofilm-derived membrane proteins |
topic_facet |
Ciencias Exactas Biofilms BipA Bordetella pertussis Proteomics Vaccination |
description |
Pertussis is an infectious respiratory disease of humans caused by the gram-negative pathogen <i>Bordetella pertussis</i>. The use of acellular pertussis vaccines (aPs) which induce immunity of relative short duration and the emergence of vaccine-adapted strains are thought to have contributed to the recent resurgence of pertussis in industrialized countries despite high vaccination coverage. Current pertussis vaccines consist of antigens derived from planktonic bacterial cultures. However, recent studies have shown that biofilm formation represents an important aspect of <i>B. pertussis</i> infection, and antigens expressed during this stage may therefore be potential targets for vaccination. Here we provide evidence that vaccination of mice with <i>B. pertussis</i> biofilm-derived membrane proteins protects against infection. Subsequent proteomic analysis of the protein content of biofilm and planktonic cultures yielded 11 proteins which were ≥ three-fold more abundant in biofilms, of which Bordetella intermediate protein A (BipA) was the most abundant, surface-exposed protein. As proof of concept, mice were vaccinated with recombinantly produced BipA. Immunization significantly reduced colonization of the lungs and antibodies to BipA were found to efficiently opsonize bacteria. Finally, we confirmed that <i>bipA</i> is expressed during respiratory tract infection of mice, and that anti-BipA antibodies are present in the serum of convalescent whooping cough patients. Together, these data suggest that biofilm proteins and in particular BipA may be of interest for inclusion into future pertussis vaccines. |
format |
Articulo Articulo |
author |
De Gouw, Daan Serra, Diego Omar Jonge, Marien I. de Hermans, Peter W. M. Wessels, Hans J. C. T. Zomer, Aldert Yantorno, Osvaldo Miguel Diavatopoulos, Dimitri A. Mooi, Frits R. |
author_facet |
De Gouw, Daan Serra, Diego Omar Jonge, Marien I. de Hermans, Peter W. M. Wessels, Hans J. C. T. Zomer, Aldert Yantorno, Osvaldo Miguel Diavatopoulos, Dimitri A. Mooi, Frits R. |
author_sort |
De Gouw, Daan |
title |
The vaccine potential of Bordetella pertussis biofilm-derived membrane proteins |
title_short |
The vaccine potential of Bordetella pertussis biofilm-derived membrane proteins |
title_full |
The vaccine potential of Bordetella pertussis biofilm-derived membrane proteins |
title_fullStr |
The vaccine potential of Bordetella pertussis biofilm-derived membrane proteins |
title_full_unstemmed |
The vaccine potential of Bordetella pertussis biofilm-derived membrane proteins |
title_sort |
vaccine potential of bordetella pertussis biofilm-derived membrane proteins |
publishDate |
2014 |
url |
http://sedici.unlp.edu.ar/handle/10915/85128 |
work_keys_str_mv |
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Repositorios |
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1764820488991801347 |