Decay-accelerating factor 1 deficiency exacerbates leptospiral-induced murine chronic nephritis and renal fibrosis

Leptospirosis is a global zoonosis caused by pathogenic Leptospira, which can colonize the proximal renal tubules and persist for long periods in the kidneys of infected hosts. Here, we characterized the infection of C57BL/6J wild-type and Daf1-/- mice, which have an enhanced host response, with a v...

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Detalles Bibliográficos
Autores principales: Ferrer, María Florencia, Scharrig, Emilia, Alberdi, Lucrecia, Cedola, Maia Tatiana, Prêtre, Gabriela, Drut, Ricardo, Song, Wen-Chao, Gómez, Ricardo Martín
Formato: Articulo
Lenguaje:Inglés
Publicado: 2014
Materias:
Biología
Leptospirosis
Murine chronic nephritis
Renal fibrosis
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/85050
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-85050
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Leptospirosis
Murine chronic nephritis
Renal fibrosis
spellingShingle Biología
Leptospirosis
Murine chronic nephritis
Renal fibrosis
Ferrer, María Florencia
Scharrig, Emilia
Alberdi, Lucrecia
Cedola, Maia Tatiana
Prêtre, Gabriela
Drut, Ricardo
Song, Wen-Chao
Gómez, Ricardo Martín
Decay-accelerating factor 1 deficiency exacerbates leptospiral-induced murine chronic nephritis and renal fibrosis
topic_facet Biología
Leptospirosis
Murine chronic nephritis
Renal fibrosis
description Leptospirosis is a global zoonosis caused by pathogenic Leptospira, which can colonize the proximal renal tubules and persist for long periods in the kidneys of infected hosts. Here, we characterized the infection of C57BL/6J wild-type and Daf1-/- mice, which have an enhanced host response, with a virulent Leptospira interrogans strain at 14 days post-infection, its persistence in the kidney, and its link to kidney fibrosis at 90 days post-infection. We found that Leptospira interrogans can induce acute moderate nephritis in wild-type mice and is able to persist in some animals, inducing fibrosis in the absence of mortality. In contrast, Daf1-/- mice showed acute mortality, with a higher bacterial burden. At the chronic stage, Daf1-/- mice showed greater inflammation and fibrosis than at 14 days post-infection and higher levels at all times than the wild-type counterpart. Compared with uninfected mice, infected wild-type mice showed higher levels of IL-4, IL-10 and IL-13, with similar levels of α-smooth muscle actin, galectin-3, TGF-β1, IL-17, IFN-γ , and lower IL-12 levels at 90 days post-infection. In contrast, fibrosis in Daf1-/- mice was accompanied by high expression of α-smooth muscle actin, galectin-3, IL-10, IL-13, and IFN-γ, similar levels of TGF-β1, IL-12, and IL-17 and lower IL-4 levels. This study demonstrates the link between Leptospira-induced murine chronic nephritis with renal fibrosis and shows a protective role of Daf1.
format Articulo
Articulo
author Ferrer, María Florencia
Scharrig, Emilia
Alberdi, Lucrecia
Cedola, Maia Tatiana
Prêtre, Gabriela
Drut, Ricardo
Song, Wen-Chao
Gómez, Ricardo Martín
author_facet Ferrer, María Florencia
Scharrig, Emilia
Alberdi, Lucrecia
Cedola, Maia Tatiana
Prêtre, Gabriela
Drut, Ricardo
Song, Wen-Chao
Gómez, Ricardo Martín
author_sort Ferrer, María Florencia
title Decay-accelerating factor 1 deficiency exacerbates leptospiral-induced murine chronic nephritis and renal fibrosis
title_short Decay-accelerating factor 1 deficiency exacerbates leptospiral-induced murine chronic nephritis and renal fibrosis
title_full Decay-accelerating factor 1 deficiency exacerbates leptospiral-induced murine chronic nephritis and renal fibrosis
title_fullStr Decay-accelerating factor 1 deficiency exacerbates leptospiral-induced murine chronic nephritis and renal fibrosis
title_full_unstemmed Decay-accelerating factor 1 deficiency exacerbates leptospiral-induced murine chronic nephritis and renal fibrosis
title_sort decay-accelerating factor 1 deficiency exacerbates leptospiral-induced murine chronic nephritis and renal fibrosis
publishDate 2014
url http://sedici.unlp.edu.ar/handle/10915/85050
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