Characterization of AbiR, a novel multicomponent abortive infection mechanism encoded by plasmid pKR223 of Lactococcus lactis subsp. lactis KR2

The native lactococcal plasmid pKR223 encodes two distinct phage resistance mechanisms, a restriction and modification (R/M) system designated <i>Lla</i>KR2I and an abortive infection mechanism (Abi) which affects prolate- headed-phage proliferation. The nucleotide sequence of a 16,174-b...

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Autores principales: Twomey, Denis P., Urraza, Patricio José de, McKay, Larry L., O'Sullivan, Daniel J.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2000
Materias:
Ciencias Exactas
Lactococcus lactis
plasmid pKR223
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/84885
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-84885
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Lactococcus lactis
plasmid pKR223
spellingShingle Ciencias Exactas
Lactococcus lactis
plasmid pKR223
Twomey, Denis P.
Urraza, Patricio José de
McKay, Larry L.
O'Sullivan, Daniel J.
Characterization of AbiR, a novel multicomponent abortive infection mechanism encoded by plasmid pKR223 of Lactococcus lactis subsp. lactis KR2
topic_facet Ciencias Exactas
Lactococcus lactis
plasmid pKR223
description The native lactococcal plasmid pKR223 encodes two distinct phage resistance mechanisms, a restriction and modification (R/M) system designated <i>Lla</i>KR2I and an abortive infection mechanism (Abi) which affects prolate- headed-phage proliferation. The nucleotide sequence of a 16,174-bp segment of pKR223 encompassing both the R/M and Abi determinants has been determined, and sequence analysis has validated the novelty of the Abi system, which has now been designated AbiR. Analysis of deletion and insertion clones demonstrated that AbiR was encoded by two genetic loci, separated by the <i>Lla</i>KR2I R/M genes. Mechanistic studies on the AbiR phenotype indicated that it was heat sensitive and that it impeded phage DNA replication. These data indicated that AbiR is a novel multicomponent, heat-sensitive, 'early'- functioning Abi system and is the first lactococcal Abi system described which is encoded by two separated genetic loci.
format Articulo
Articulo
author Twomey, Denis P.
Urraza, Patricio José de
McKay, Larry L.
O'Sullivan, Daniel J.
author_facet Twomey, Denis P.
Urraza, Patricio José de
McKay, Larry L.
O'Sullivan, Daniel J.
author_sort Twomey, Denis P.
title Characterization of AbiR, a novel multicomponent abortive infection mechanism encoded by plasmid pKR223 of Lactococcus lactis subsp. lactis KR2
title_short Characterization of AbiR, a novel multicomponent abortive infection mechanism encoded by plasmid pKR223 of Lactococcus lactis subsp. lactis KR2
title_full Characterization of AbiR, a novel multicomponent abortive infection mechanism encoded by plasmid pKR223 of Lactococcus lactis subsp. lactis KR2
title_fullStr Characterization of AbiR, a novel multicomponent abortive infection mechanism encoded by plasmid pKR223 of Lactococcus lactis subsp. lactis KR2
title_full_unstemmed Characterization of AbiR, a novel multicomponent abortive infection mechanism encoded by plasmid pKR223 of Lactococcus lactis subsp. lactis KR2
title_sort characterization of abir, a novel multicomponent abortive infection mechanism encoded by plasmid pkr223 of lactococcus lactis subsp. lactis kr2
publishDate 2000
url http://sedici.unlp.edu.ar/handle/10915/84885
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