Differentiation of Paenibacillus larvae subsp. larvae, the cause of american foulbrood of honeybees, by using PCR and restriction fragment analysis of genes encoding 16S rRNA

A rapid procedure for the identification of <i>Paenibacillus larvae</i> subsp. <i>larvae</i>, the causal agent of American foulbrood (AFB) disease of honeybees (<i>Apis mellifera</i> L.), based on PCR and restriction fragment analysis of the 16S rRNA genes (rDNA)...

Descripción completa

Detalles Bibliográficos
Autores principales: Alippi, Adriana Mónica, López, Ana Claudia, Aguilar, Orlando Mario
Formato: Articulo
Lenguaje:Inglés
Publicado: 2002
Materias:
Ciencias Agrarias
Ciencias Exactas
Paenibacillus larvae
honeybees
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/84655
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-84655
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Agrarias
Ciencias Exactas
Paenibacillus larvae
honeybees
spellingShingle Ciencias Agrarias
Ciencias Exactas
Paenibacillus larvae
honeybees
Alippi, Adriana Mónica
López, Ana Claudia
Aguilar, Orlando Mario
Differentiation of Paenibacillus larvae subsp. larvae, the cause of american foulbrood of honeybees, by using PCR and restriction fragment analysis of genes encoding 16S rRNA
topic_facet Ciencias Agrarias
Ciencias Exactas
Paenibacillus larvae
honeybees
description A rapid procedure for the identification of <i>Paenibacillus larvae</i> subsp. <i>larvae</i>, the causal agent of American foulbrood (AFB) disease of honeybees (<i>Apis mellifera</i> L.), based on PCR and restriction fragment analysis of the 16S rRNA genes (rDNA) is described. Eighty-six bacterial strains belonging to 39 species of the genera <i>Paenibacillus</i>, <i>Bacillus</i>, <i>Brevibacillus</i>, and <i>Virgibacillus</i> were characterized. Amplified rDNA was digested with seven restriction endonucleases. The combined data from restriction analysis enabled us to distinguish 35 profiles. Cluster analysis revealed that <i>P. larvae</i> subsp. <i>larvae</i> and Paenibacillus larvae subsp. <i>pulvifaciens</i> formed a group with about 90% similarity; however, the <i>P. larvae</i> subsp. <i>larvae</i> restriction fragment length polymorphism pattern produced by endonuclease <i>Hae</i>III was found to be unique and distinguishable among other closely related bacteria. This pattern was associated with DNA extracted directly from honeybee brood samples showing positive AFB clinical signs that yielded the restriction profile characteristic of <i>P. larvae</i> subsp. <i>larvae</i>, while no amplification product was obtained from healthy larvae. The method described here is particularly useful because of the short time required to carry it out and because it allows the differentiation of <i>P. larvae</i> subsp. <i>larvae</i>-infected larvae from all other species found in apiarian sources.
format Articulo
Articulo
author Alippi, Adriana Mónica
López, Ana Claudia
Aguilar, Orlando Mario
author_facet Alippi, Adriana Mónica
López, Ana Claudia
Aguilar, Orlando Mario
author_sort Alippi, Adriana Mónica
title Differentiation of Paenibacillus larvae subsp. larvae, the cause of american foulbrood of honeybees, by using PCR and restriction fragment analysis of genes encoding 16S rRNA
title_short Differentiation of Paenibacillus larvae subsp. larvae, the cause of american foulbrood of honeybees, by using PCR and restriction fragment analysis of genes encoding 16S rRNA
title_full Differentiation of Paenibacillus larvae subsp. larvae, the cause of american foulbrood of honeybees, by using PCR and restriction fragment analysis of genes encoding 16S rRNA
title_fullStr Differentiation of Paenibacillus larvae subsp. larvae, the cause of american foulbrood of honeybees, by using PCR and restriction fragment analysis of genes encoding 16S rRNA
title_full_unstemmed Differentiation of Paenibacillus larvae subsp. larvae, the cause of american foulbrood of honeybees, by using PCR and restriction fragment analysis of genes encoding 16S rRNA
title_sort differentiation of paenibacillus larvae subsp. larvae, the cause of american foulbrood of honeybees, by using pcr and restriction fragment analysis of genes encoding 16s rrna
publishDate 2002
url http://sedici.unlp.edu.ar/handle/10915/84655
work_keys_str_mv AT alippiadrianamonica differentiationofpaenibacilluslarvaesubsplarvaethecauseofamericanfoulbroodofhoneybeesbyusingpcrandrestrictionfragmentanalysisofgenesencoding16srrna
AT lopezanaclaudia differentiationofpaenibacilluslarvaesubsplarvaethecauseofamericanfoulbroodofhoneybeesbyusingpcrandrestrictionfragmentanalysisofgenesencoding16srrna
AT aguilarorlandomario differentiationofpaenibacilluslarvaesubsplarvaethecauseofamericanfoulbroodofhoneybeesbyusingpcrandrestrictionfragmentanalysisofgenesencoding16srrna
bdutipo_str Repositorios
_version_ 1764820489070444545

Ejemplares similares