Effects of chemical ischemia on purine nucleotides, free radical generation, lipids peroxidation and intracellular calcium levels in C 2C12 myotube derived from mouse myocytes

To elucidate the mechanisms of ischemia-mediated myopathy using <i>in vitro</i> model, changes of purine nucleotides, membrane lipid peroxidation(TBARS), intracellular calcium ([Ca<SUP>2+</SUP>]i)levels, generation of free radicals, and deoxyribonucleic acid (DNA) fragmentati...

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Autores principales: Boffi, Federico Martín, Ozaki, Junichiro, Matsuki, Naoaki, Inaba, Mutsumi, Desmaras, Eduardo Armando, Ono, Kenichiro
Formato: Articulo
Lenguaje:Inglés
Publicado: 2002
Materias:
Ciencias Veterinarias
Apoptotic pathway
C2C12 myotube
Chemical ischemia
Free radical generation
Purine nucleotide
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/84626
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-84626
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Apoptotic pathway
C2C12 myotube
Chemical ischemia
Free radical generation
Purine nucleotide
spellingShingle Ciencias Veterinarias
Apoptotic pathway
C2C12 myotube
Chemical ischemia
Free radical generation
Purine nucleotide
Boffi, Federico Martín
Ozaki, Junichiro
Matsuki, Naoaki
Inaba, Mutsumi
Desmaras, Eduardo Armando
Ono, Kenichiro
Effects of chemical ischemia on purine nucleotides, free radical generation, lipids peroxidation and intracellular calcium levels in C 2C12 myotube derived from mouse myocytes
topic_facet Ciencias Veterinarias
Apoptotic pathway
C2C12 myotube
Chemical ischemia
Free radical generation
Purine nucleotide
description To elucidate the mechanisms of ischemia-mediated myopathy using <i>in vitro</i> model, changes of purine nucleotides, membrane lipid peroxidation(TBARS), intracellular calcium ([Ca<SUP>2+</SUP>]i)levels, generation of free radicals, and deoxyribonucleic acid (DNA) fragmentation were examined in mouse-derived C<SUB>2</SUB>C<SUB>12</SUB> myotubes under the condition with an inhibition of glycolytic and oxidative metabolism as the ischemic condition. In purine nucleotides, intracellular adenosine triphosphate (ATP) and guanosine triphosphate (GTP) concentrations rapidly and significantly decreased after the treatment with ischemia. No remarkable differences were observed in other purine nucleotides, with the exception of inosine monophosphate (IMP) and extracellular hypoxanthine levels, both of which increased significantly during the ischemia. The lactate dehydrogenase activity in culture supernatant of C<SUB>2</SUB>C<SUB>12</SUB> myotubes increased significantly from 2 to 4 hr after the ischemia. On the generation of free radicals, no spectrum was detected in supernatants throughout the observation period, whereas supernatant TBARS concentration increased rapidly and significantly after the ischemia. The relative intensity of [Ca<SUP>2+</SUP>]i significantly increased after the ischemia. On the fragmented deoxyribonucleic acid(DNA), no TUNEL positive cells was detected in C<SUB>2</SUB>C<SUB>12</SUB> myotubes after 1 hr of the ischemia, however the positive cell percentage subsequently increased. From these results, it was suggested that the ischemic condition induced changes of membrane permeability and increase of [Ca<SUP>2+</SUP>]i, both of which lead to cell membrane damage, although a free radical generation was not detected. The ischemic condition also induced the release of substrate hypoxanthine for free radical generation and might initiate the apoptotic pathway in C<SUB>2</SUB>C<SUB>12</SUB> myotubes.
format Articulo
Articulo
author Boffi, Federico Martín
Ozaki, Junichiro
Matsuki, Naoaki
Inaba, Mutsumi
Desmaras, Eduardo Armando
Ono, Kenichiro
author_facet Boffi, Federico Martín
Ozaki, Junichiro
Matsuki, Naoaki
Inaba, Mutsumi
Desmaras, Eduardo Armando
Ono, Kenichiro
author_sort Boffi, Federico Martín
title Effects of chemical ischemia on purine nucleotides, free radical generation, lipids peroxidation and intracellular calcium levels in C 2C12 myotube derived from mouse myocytes
title_short Effects of chemical ischemia on purine nucleotides, free radical generation, lipids peroxidation and intracellular calcium levels in C 2C12 myotube derived from mouse myocytes
title_full Effects of chemical ischemia on purine nucleotides, free radical generation, lipids peroxidation and intracellular calcium levels in C 2C12 myotube derived from mouse myocytes
title_fullStr Effects of chemical ischemia on purine nucleotides, free radical generation, lipids peroxidation and intracellular calcium levels in C 2C12 myotube derived from mouse myocytes
title_full_unstemmed Effects of chemical ischemia on purine nucleotides, free radical generation, lipids peroxidation and intracellular calcium levels in C 2C12 myotube derived from mouse myocytes
title_sort effects of chemical ischemia on purine nucleotides, free radical generation, lipids peroxidation and intracellular calcium levels in c 2c12 myotube derived from mouse myocytes
publishDate 2002
url http://sedici.unlp.edu.ar/handle/10915/84626
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